{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T23:13:47Z","timestamp":1751411627651},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,4,16]],"date-time":"2016-04-16T00:00:00Z","timestamp":1460764800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To evaluate the immunoexpression of tryptase, <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20109 and <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u201013 in periapical lesions, correlating them with the type of lesion, intensity of the inflammatory infiltrate and thickness of the epithelial lining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>Twenty periapical granulomas (<jats:styled-content style=\"fixed-case\">PG<\/jats:styled-content>s), twenty radicular cysts (<jats:styled-content style=\"fixed-case\">RC<\/jats:styled-content>s) and twenty residual radicular cysts (<jats:styled-content style=\"fixed-case\">RRC<\/jats:styled-content>s) were submitted to immunohistochemical analysis using antitryptase, anti\u2010<jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20109 and anti\u2010<jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u201013 antibodies. Immunoexpression of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20109 and <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u201013 was quantitatively evaluated both in the connective tissue of all lesions and in the epithelial lining of <jats:styled-content style=\"fixed-case\">RC<\/jats:styled-content>s and <jats:styled-content style=\"fixed-case\">RRC<\/jats:styled-content>s. Tryptase\u2010positive mast cells were counted only in the connective tissue. The data were analysed using Kruskal\u2013Wallis and Fisher's exact tests, as well as Spearman's correlation test (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In comparison with <jats:styled-content style=\"fixed-case\">RC<\/jats:styled-content>s and <jats:styled-content style=\"fixed-case\">RRC<\/jats:styled-content>s, <jats:styled-content style=\"fixed-case\">PG<\/jats:styled-content>s exhibited higher immunoexpression of tryptase, <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20109 and <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u201013 (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.002, <jats:italic>P<\/jats:italic> = &lt; 0.001 and <jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.001, respectively). In comparison with lesions with inflammatory infiltrates grades I and <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>, lesions with inflammatory infiltrate grade <jats:styled-content style=\"fixed-case\">III<\/jats:styled-content> had higher median percentages of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u201013\u2010positive cells (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.003) and a tendency for higher expression of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20109 (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.059). No significant difference was observed between the expression of the studied markers and epithelial thickness (<jats:italic>P\u00a0<\/jats:italic>&gt;<jats:italic>\u00a0<\/jats:italic>0.05). There were positive correlations between the number of tryptase\u2010positive mast cells and the immunoexpression of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20109, as well as between the immunoexpression of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20109 and <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u201013.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>A larger number of tryptase\u2010positive mast cells and greater enzymatic activity of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20109 and <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u201013 were found in <jats:styled-content style=\"fixed-case\">PG<\/jats:styled-content>s compared to <jats:styled-content style=\"fixed-case\">RC<\/jats:styled-content>s and <jats:styled-content style=\"fixed-case\">RRC<\/jats:styled-content>s. These findings are a characteristic of the dynamics of periapical diseases.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.12638","type":"journal-article","created":{"date-parts":[[2016,3,22]],"date-time":"2016-03-22T12:27:28Z","timestamp":1458649648000},"page":"446-454","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Analysis of tryptase\u2010positive mast cells and immunoexpression of <scp>MMP<\/scp>\u20109 and <scp>MMP<\/scp>\u201013 in periapical lesions"],"prefix":"10.1111","volume":"50","author":[{"given":"A. L. D. L.","family":"Andrade","sequence":"first","affiliation":[{"name":"Department of Dentistry Federal University of Rio Grande do Norte Natal Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. 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