{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T17:29:35Z","timestamp":1790270975151,"version":"4.1.0"},"reference-count":25,"publisher":"Wiley","issue":"S4","license":[{"start":{"date-parts":[[2017,2,23]],"date-time":"2017-02-23T00:00:00Z","timestamp":1487808000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100009567","name":"Conseil R\u00e9gional de Franche-Comt\u00e9","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100009567","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>To isolate and characterize dental pulp stem cells (DPSCs) obtained from carious and healthy mature teeth extracted when conservative treatment was not possible or for orthodontic reasons; to evaluate the ability of DPSCs to colonize, proliferate and differentiate into functional odontoblast\u2010like cells when cultured onto a polycaprolactone cone made by jet\u2010spraying and prototyped into a design similar to a gutta\u2010percha cone.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methodology<\/jats:title>\n                    <jats:p>\n                      DPSCs were obtained from nine carious and 12 healthy mature teeth. Then cells were characterized by flow cytometry and submitted to multidifferentiation to confirm their multipotency. These DPSCs were then cultured on a polycaprolactone cone in an odontoblastic differentiation medium. Cell proliferation, colonization of the biomaterial and functional differentiation of cells were histologically assessed. For the characterization, a\n                      <jats:italic>t<\/jats:italic>\n                      \u2010Student test was used to compare the two groups.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In all cell cultures, characterization highlighted a mesenchymal stem cell phenotype (CD105+, CD90+, CD73+, CD11b\u2212, CD34\u2212, CD45\u2212, HLA\u2010DR\u2212). No significant differences were found between cultures obtained from carious and healthy mature teeth. DPSCs from both origins were able to differentiate into osteocytes, adipocytes and chondrocytes. Cell colonization was observed both on the surface and in the thickness of polycaprolactone cones as well as a mineralized pericellular matrix deposit composed of type I collagen, alkaline phosphatase, osteocalcin and dentin sialophosphoprotein.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>DPSCs were isolated from both carious and healthy mature teeth. They were able to colonize and proliferate within a polycaprolactone cone and could be differentiated into functional odontoblast\u2010like cells.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/iej.12746","type":"journal-article","created":{"date-parts":[[2017,1,21]],"date-time":"2017-01-21T09:02:42Z","timestamp":1484989362000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Odontoblastic differentiation of dental pulp stem cells from healthy and carious teeth on an original PCL\u2010based 3D scaffold"],"prefix":"10.1111","volume":"51","author":[{"given":"A.","family":"Louvrier","sequence":"first","affiliation":[{"name":"Service de Chirurgie Maxillo\u2010faciale Stomatologie et Odontologie Hospitali\u00e8re Centre Hospitalier Universitaire de Besan\u00e7on Besan\u00e7on France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Euvrard","sequence":"additional","affiliation":[{"name":"Service de Chirurgie Maxillo\u2010faciale Stomatologie et Odontologie Hospitali\u00e8re Centre Hospitalier Universitaire de Besan\u00e7on Besan\u00e7on France"},{"name":"Nanom\u00e9decinelab, EA 4662 UFR SMP Universit\u00e9 de Franche\u2010Comt\u00e9 Besan\u00e7on France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Nicod","sequence":"additional","affiliation":[{"name":"Nanom\u00e9decinelab, EA 4662 UFR SMP Universit\u00e9 de Franche\u2010Comt\u00e9 Besan\u00e7on France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Rolin","sequence":"additional","affiliation":[{"name":"Centre d'Investigation Clinique de Besan\u00e7on INSERM 1431 Centre Hospitalier R\u00e9gional Universitaire de Besan\u00e7on Besan\u00e7on France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Gindraux","sequence":"additional","affiliation":[{"name":"Nanom\u00e9decinelab, EA 4662 UFR SMP Universit\u00e9 de Franche\u2010Comt\u00e9 Besan\u00e7on France"},{"name":"Service de Chirurgie Orthop\u00e9dique, Traumatologique et Plastique Centre Hospitalier Universitaire de Besan\u00e7on Besan\u00e7on France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Pazart","sequence":"additional","affiliation":[{"name":"Centre d'Investigation Clinique de Besan\u00e7on INSERM 1431 Centre Hospitalier R\u00e9gional Universitaire de Besan\u00e7on Besan\u00e7on France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Houdayer","sequence":"additional","affiliation":[{"name":"Estrog\u00e8nes Expression G\u00e9nique et Pathologies du Syst\u00e8me Nerveux Central EA3922 UFR ST Universit\u00e9 de Franche\u2010Comt\u00e9 Besan\u00e7on France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. 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