{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T01:02:32Z","timestamp":1784941352469,"version":"3.55.0"},"reference-count":34,"publisher":"Wiley","issue":"S2","license":[{"start":{"date-parts":[[2017,3,17]],"date-time":"2017-03-17T00:00:00Z","timestamp":1489708800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["RD12\/0019\/0001"],"award-info":[{"award-number":["RD12\/0019\/0001"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2017,12]]},"abstract":"Abstract<\/jats:title>Aims<\/jats:title>To investigate the cytotoxicity and bioactivity of several pulpotomy materials: Biodentine (Septodont, Saint\u2010Maur\u2010des\u2010Fosses, France) MTA<\/jats:styled-content> (Angelus, Londrina, PR<\/jats:styled-content>, Brazil), Theracal LC<\/jats:styled-content> (Bisco Inc., Schamburg, IL<\/jats:styled-content>, USA<\/jats:styled-content>) and IRM<\/jats:styled-content> (Dentsply DeTrey GmbH, Konstanz, Germany), after contact with stem cells isolated from human exfoliated primary teeth (SHED<\/jats:styled-content>s).<\/jats:p><\/jats:sec>Methodology<\/jats:title>SHED<\/jats:styled-content>s were cultured in the presence of the eluates of various pulpotomy materials for 24, 48 and 72\u00a0h. Cell viability was determined by mitochondrial dehydrogenase enzymatic (MTT<\/jats:styled-content>) assay. Apoptosis and changes in cell phenotype were evaluated by flow cytometry. Also, an in\u00a0vitro<\/jats:italic> scratch wound\u2010healing assay was used to determine their effects on cell migration. To assess cell morphology and attachment to the different pulpotomy materials, SHED<\/jats:styled-content>s were directly seeded onto the material surfaces and analysed by scanning electron microscopy (SEM<\/jats:styled-content>). Finally, the deposition of a calcified matrix in presence of these materials was verified by Alizarin Red staining. Statistical analysis was performed with analysis of variance and Bonferroni or Tukey post\u2010test (\u03b1\u00a0=\u00a00.05).<\/jats:p><\/jats:sec>Results<\/jats:title>Cell viability in the presence of Biodentine eluates was significantly higher to that obtained using complete medium alone (control; P<\/jats:italic>\u00a0<\u00a00.01) and was also significantly higher than using MTA<\/jats:styled-content> Angelus from 48\u00a0h of incubation (P<\/jats:italic>\u00a0<\u00a00.01). However, Theracal LC<\/jats:styled-content> and IRM<\/jats:styled-content> were associated with low rates of cell viability (P<\/jats:italic>\u00a0<\u00a00.001). Similar results were obtained in an apoptosis assay. In addition, SHED<\/jats:styled-content>s maintained their mesenchymal phenotype in all conditions although their capacity to migrate was higher in the presence of Biodentine. SEM<\/jats:styled-content> studies revealed a suitable proliferation rate, cell spreading and attachment, especially when using Biodentine and MTA<\/jats:styled-content> Angelus discs. Finally, Biodentine eluates significantly induced calcified matrix deposition from 7\u00a0days of culture (P<\/jats:italic>\u00a0<\u00a00.01).<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Biodentine exhibited better cytocompatibility and bioactivity than MTA<\/jats:styled-content> Angelus, Theracal LC<\/jats:styled-content> and IRM<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.12751","type":"journal-article","created":{"date-parts":[[2017,2,7]],"date-time":"2017-02-07T12:06:47Z","timestamp":1486469207000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":65,"title":["Cytotoxicity and bioactivity of various pulpotomy materials on stem cells from human exfoliated primary teeth"],"prefix":"10.1111","volume":"50","author":[{"given":"M.","family":"Collado\u2010Gonz\u00e1lez","sequence":"first","affiliation":[{"name":"Cellular Therapy and Hematopoietic Transplant Unit Hematology Department Virgen de la Arrixaca Clinical University Hospital IMIB University of Murcia Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Garc\u00eda\u2010Bernal","sequence":"additional","affiliation":[{"name":"Cellular Therapy and Hematopoietic Transplant Unit Hematology Department Virgen de la Arrixaca Clinical University Hospital IMIB University of Murcia Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. E.","family":"O\u00f1ate\u2010S\u00e1nchez","sequence":"additional","affiliation":[{"name":"School of Dentistry Faculty of Medicine University of Murcia Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. S.","family":"Ortolani\u2010Seltenerich","sequence":"additional","affiliation":[{"name":"Department of Stomatology Universitat de Valencia Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"\u00c1lvarez\u2010Muro","sequence":"additional","affiliation":[{"name":"Dental Clinic Esdens Alicante Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Lozano","sequence":"additional","affiliation":[{"name":"Department of Stomatology Universitat de Valencia Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Forner","sequence":"additional","affiliation":[{"name":"Department of Stomatology Universitat de Valencia Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Llena","sequence":"additional","affiliation":[{"name":"Department of Stomatology Universitat de Valencia Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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