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The expressions of molecules involved in physiological (<jats:italic>alkaline phosphatase<\/jats:italic>,<jats:italic> type I collagen<\/jats:italic>) and in inflammatory processes (<jats:italic><jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">CXCL<\/jats:styled-content>8<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">CCL<\/jats:styled-content>20<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">COX<\/jats:styled-content>\u20102<\/jats:italic>) were studied using real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. Concentrations of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106 and <jats:styled-content style=\"fixed-case\">CXCL<\/jats:styled-content>8 were analysed during 4\u00a0days with <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>. Nonparametric tests were used to determine statistical differences between groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A significant decrease (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.001) in <jats:italic>type I collagen<\/jats:italic> and <jats:italic>alkaline phosphatase<\/jats:italic> was observed in <jats:styled-content style=\"fixed-case\">MRC<\/jats:styled-content>5 and <jats:styled-content style=\"fixed-case\">HDPF<\/jats:styled-content> nemotic responses. Although the amounts of <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> differed between these cell types, there was an increase in <jats:italic><jats:styled-content style=\"fixed-case\">CCL<\/jats:styled-content>20<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">CXCL<\/jats:styled-content>8<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">COX<\/jats:styled-content>\u20102<\/jats:italic> expression (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.001). Unlike <jats:styled-content style=\"fixed-case\">HDPF<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> MRC<\/jats:styled-content>5 spheroids displayed significant amounts of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106 concentrations and <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression. Notably, increased concentrations of <jats:styled-content style=\"fixed-case\">CXCL<\/jats:styled-content>8 were recorded in all three\u2010dimensional cultures compared with monolayers as a function of time (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Although the nemotic responses observed were not identical in the pulpal and lung fibroblasts, similarities occurred in the expression of chemokines and cyclooxygenase\u20102. Nemotic reactions and inflammatory processes in pulp diseases share similarities in terms of the expression of factors. Thus, this <jats:italic>in\u00a0vitro<\/jats:italic> model could constitute a powerful tool to study intercellular relations within the dental pulp and to develop new local treatments to counteract the inflammatory reaction that occurs during pulpitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.12762","type":"journal-article","created":{"date-parts":[[2017,3,10]],"date-time":"2017-03-10T17:40:44Z","timestamp":1489167644000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Expression of factors involved in dental pulp physiopathological processes by nemotic human pulpal fibroblasts"],"prefix":"10.1111","volume":"51","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6025-8167","authenticated-orcid":false,"given":"J.","family":"Le Clerc","sequence":"first","affiliation":[{"name":"Facult\u00e9 d'Odontologie Equipe Verres et C\u00e9ramiques UMR CNRS 6226 Institut des Sciences Chimiques de Rennes, Universit\u00e9 de 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