{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T18:52:27Z","timestamp":1789757547426,"version":"4.0.1"},"reference-count":50,"publisher":"Wiley","issue":"S2","license":[{"start":{"date-parts":[[2017,5,2]],"date-time":"2017-05-02T00:00:00Z","timestamp":1493683200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"State of S\u00e3o Paulo","award":["2015\/03437-6 and 2014\/13353-1"],"award-info":[{"award-number":["2015\/03437-6 and 2014\/13353-1"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2017,12]]},"abstract":"Abstract<\/jats:title>\n \n Aim<\/jats:title>\n \n To evaluate the biocompatibility and mineralized nodule formation of an experimental tricalcium silicate cement with tantalum oxide (\n TSC<\/jats:styled-content>\n \/Ta\n 2<\/jats:sub>\n O\n 5<\/jats:sub>\n ) as radiopacifier, Neo\n MTA<\/jats:styled-content>\n Plus (Avalon Biomed Inc., Bradenton,\n FL<\/jats:styled-content>\n ,\n USA<\/jats:styled-content>\n ) and\n MTA<\/jats:styled-content>\n (Angelus, Londrina,\n PR<\/jats:styled-content>\n , Brazil) on human osteoblast\u2010like cells (Saos\u20102).\n <\/jats:p>\n <\/jats:sec>\n \n Methodology<\/jats:title>\n \n Biocompatibility was evaluated by 3\u2010(4,5\u2010dimethyl\u2010thiazoyl)\u20102,5\u2010diphenyl\u2010tetrazolium bromide (\n MTT<\/jats:styled-content>\n ) and neutral red (\n NR<\/jats:styled-content>\n ) assays, after exposure of Saos\u20102 to cement extracts at 1\u00a0:\u00a01, 1\u00a0:\u00a02, 1\u00a0:\u00a04 and 1\u00a0:\u00a08 dilutions for 24\u00a0h. Bioactivity was evaluated by alkaline phosphatase (\n ALP<\/jats:styled-content>\n ) activity, and calcium deposits were detected with alizarin red staining (\n ARS<\/jats:styled-content>\n ). Statistical analysis was performed with analysis of variance and Bonferroni or Tukey post\u2010test (\u03b1\u00a0=\u00a00.05).\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The\n MTT<\/jats:styled-content>\n assay revealed lower cytotoxicity for\n NEO<\/jats:styled-content>\n and\n MTA<\/jats:styled-content>\n (\n P<\/jats:italic>\n \u00a0<\u00a00.05), and higher for\n TSC<\/jats:styled-content>\n \/Ta\n 2<\/jats:sub>\n O\n 5<\/jats:sub>\n at 1\u00a0:\u00a01 and 1\u00a0:\u00a02 dilutions when compared to serum\u2010free medium \u2013 control (\n P<\/jats:italic>\n \u00a0>\u00a00.05). At 1\u00a0:\u00a04 dilution, the\n TSC<\/jats:styled-content>\n \/Ta\n 2<\/jats:sub>\n O\n 5<\/jats:sub>\n cytotoxicity was similar to the control (\n P<\/jats:italic>\n \u00a0>\u00a00.05). At 1\u00a0:\u00a08 dilution, cell viability was significantly greater than the control (\n P<\/jats:italic>\n \u00a0<\u00a00.05). Saos\u20102 cell viability performed using the\n NR<\/jats:styled-content>\n assay at all dilutions revealed no cytotoxic effect of\n MTA<\/jats:styled-content>\n ,\n NEO<\/jats:styled-content>\n and\n TSC<\/jats:styled-content>\n \/Ta\n 2<\/jats:sub>\n O\n 5<\/jats:sub>\n .\n ALP<\/jats:styled-content>\n activity at 1 and 3\u00a0days was similar to the control (\n P<\/jats:italic>\n \u00a0>\u00a00.05).\n TSC<\/jats:styled-content>\n \/Ta\n 2<\/jats:sub>\n O\n 5<\/jats:sub>\n had significantly greater\n ALP<\/jats:styled-content>\n activity at 7\u00a0days when compared with the control (\n P<\/jats:italic>\n \u00a0<\u00a00.05). All materials induced the production of mineralized nodules, and\n NEO<\/jats:styled-content>\n produced significantly more mineralized nodules than\n MTA<\/jats:styled-content>\n and\n TSC<\/jats:styled-content>\n \/Ta\n 2<\/jats:sub>\n O\n 5<\/jats:sub>\n (\n P<\/jats:italic>\n \u00a0<\u00a00.05).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n Neo\n MTA<\/jats:styled-content>\n Plus and\n TSC<\/jats:styled-content>\n \/Ta\n 2<\/jats:sub>\n O\n 5<\/jats:sub>\n were biocompatible and induced\n ALP<\/jats:styled-content>\n activity in Saos\u20102 cells. Both materials induced mineralized nodule formation by Saos\u20102 with Neo\n MTA<\/jats:styled-content>\n Plus producing significantly more.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/iej.12780","type":"journal-article","created":{"date-parts":[[2017,4,8]],"date-time":"2017-04-08T02:16:42Z","timestamp":1491617802000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["Biocompatibility and mineralized nodule formation of Neo MTA Plus and an experimental tricalcium silicate cement containing tantalum oxide"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2574-4706","authenticated-orcid":false,"given":"M.","family":"Tanomaru\u2010Filho","sequence":"first","affiliation":[{"name":"UNESP \u2010 S\u00e3o Paulo State University Araraquara School of Dentistry Department of Restorative Dentistry Araraquara S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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S.","family":"Viola","sequence":"additional","affiliation":[{"name":"UNESP \u2010 S\u00e3o Paulo State University Araraquara School of Dentistry Department of Restorative Dentistry Araraquara S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7030-3718","authenticated-orcid":false,"given":"G.","family":"Faria","sequence":"additional","affiliation":[{"name":"UNESP \u2010 S\u00e3o Paulo State University Araraquara School of Dentistry Department of Restorative Dentistry Araraquara S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Camilleri","sequence":"additional","affiliation":[{"name":"University of Malta Faculty of Dental Surgery Department of Restorative Dentistry Msida Malta"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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