{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:51:50Z","timestamp":1787014310930,"version":"build-2736575974"},"reference-count":54,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,7,15]],"date-time":"2017-07-15T00:00:00Z","timestamp":1500076800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100002050","name":"Delta Dental Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100002050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2018,1]]},"abstract":"Abstract<\/jats:title>\n \n Aim<\/jats:title>\n \n To evaluate Save\u2010A\u2010Tooth (\n SAT<\/jats:styled-content>\n ),\n EMT<\/jats:styled-content>\n Toothsaver (\n EMT<\/jats:styled-content>\n ) and Hank's Balanced Salt Solution (\n HBSS<\/jats:styled-content>\n ) for their influence on the viability and proliferative capacity of human periodontal ligament fibroblasts (HPDLFs).\n <\/jats:p>\n <\/jats:sec>\n \n Methodology<\/jats:title>\n \n Primary\n HPDLF<\/jats:styled-content>\n s were seeded into 96\u2010well cell culture plates and exposed to\n SAT<\/jats:styled-content>\n ,\n EMT<\/jats:styled-content>\n ,\n HBSS<\/jats:styled-content>\n and water (negative control) for 0.5, 1, 3, 6, 12 and 24\u00a0h at room temperature (22\u00a0\u00b0C). After each exposure time, cell viability was measured through quantifying adenosine triphosphate (\n ATP<\/jats:styled-content>\n ) using a luminescent dye. The proliferative capacity was also quantified using the PrestoBlue assay after 12 or 24\u00a0h storage in each medium. The data were analysed statistically by two\u2010way\n anova<\/jats:sc>\n and\n post hoc<\/jats:italic>\n Least Significant Difference (\n LSD<\/jats:styled-content>\n ) test (\n P\u00a0<\/jats:italic>\n <\n \u00a0<\/jats:italic>\n 0.05). The morphology of the cells after 12\u00a0h storage was also investigated through live\/dead viability\/cytotoxicity kit together with fluorescence microscopy.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n There was no significant difference in cell viability amongst\n HBSS<\/jats:styled-content>\n ,\n SAT<\/jats:styled-content>\n and\n EMT<\/jats:styled-content>\n groups up to 6\u00a0h.\n SAT<\/jats:styled-content>\n was effective in maintaining cell viability only up to 12\u00a0h and then became detrimental to\n HPDLF<\/jats:styled-content>\n ; after 24\u00a0h, the effectiveness of\n SAT<\/jats:styled-content>\n in maintaining cell viability was similar to that of water (\n P<\/jats:italic>\n \u00a0>\u00a00.05). Amongst all the media, only\n EMT<\/jats:styled-content>\n could maintain the proliferative capacity of\n HPDLF<\/jats:styled-content>\n s significantly higher than the negative control, that is water (\n P<\/jats:italic>\n \u00a0<\u00a00.05) after 24\u00a0h storage.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n EMT<\/jats:styled-content>\n maintained the proliferative capacity of\n HPDLF<\/jats:styled-content>\n s after 24\u00a0h storage.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/iej.12798","type":"journal-article","created":{"date-parts":[[2017,6,20]],"date-time":"2017-06-20T03:56:43Z","timestamp":1497931003000},"page":"58-68","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["The efficacy of commercial tooth storage media for maintaining the viability of human periodontal ligament fibroblasts"],"prefix":"10.1111","volume":"51","author":[{"given":"W.","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Surgical Services School of Dentistry Marquette University Milwaukee WI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Stover","sequence":"additional","affiliation":[{"name":"Department of Surgical Services School of Dentistry Marquette University Milwaukee WI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Rasoulianboroujeni","sequence":"additional","affiliation":[{"name":"Department of Developmental Sciences School of Dentistry Marquette University Milwaukee WI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Sherman","sequence":"additional","affiliation":[{"name":"Department of Mathematics, Statistics and Computer Science Marquette University Milwaukee WI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Fahimipour","sequence":"additional","affiliation":[{"name":"Department of Developmental Sciences School of Dentistry Marquette University Milwaukee WI USA"},{"name":"Dental Biomaterials Department School of Dentistry Tehran University of Medical Sciences Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Dashtimoghadam","sequence":"additional","affiliation":[{"name":"Department of Developmental Sciences School of Dentistry Marquette University Milwaukee WI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Zito","sequence":"additional","affiliation":[{"name":"Department of Developmental Sciences School of Dentistry Marquette University Milwaukee WI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. 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