{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T02:07:15Z","timestamp":1761962835318,"version":"3.37.3"},"reference-count":33,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,2,27]],"date-time":"2018-02-27T00:00:00Z","timestamp":1519689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007854","name":"School of Dentistry, Nihon University","doi-asserted-by":"publisher","award":["26462897"],"award-info":[{"award-number":["26462897"]}],"id":[{"id":"10.13039\/100007854","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["17K11717"],"award-info":[{"award-number":["17K11717"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2018,7]]},"abstract":"Abstract<\/jats:title>Aim<\/jats:title>To investigate the role played by silent information regulator 2 homologue 1 (SIRT<\/jats:styled-content>1) during angiogenesis of periapical periodontitis.<\/jats:p><\/jats:sec>Methodology<\/jats:title>Periapical granulomas were subjected to dual\u2010colour immunofluorescence imaging and real\u2010time polymerase chain reactions assaying the expression levels of SIRT<\/jats:styled-content>1, vascular endothelial growth factor (VEGF<\/jats:styled-content>) and VE<\/jats:styled-content>\u2010cadherin. The association between Ki\u201067 and SIRT<\/jats:styled-content>1 expression was also examined. Human umbilical vein endothelial cells (HUVEC<\/jats:styled-content>s) were treated with a combination of lipopolysaccharide and resveratrol (a SIRT<\/jats:styled-content>1 activator) or sirtinol (a SIRT<\/jats:styled-content>1 inhibitor); and the levels of mRNA<\/jats:styled-content>s encoding SIRT<\/jats:styled-content>1, VEGF<\/jats:styled-content> and VE<\/jats:styled-content>\u2010cadherin were determined. HUVEC<\/jats:styled-content> tube formation was assayed in the presence of resveratrol or sirtinol. The Mann\u2013Whitney U<\/jats:italic>\u2010test or the Tukey\u2013Kramer test was used for statistical analysis.<\/jats:p><\/jats:sec>Results<\/jats:title>Ki\u201067\u2010expressing cells, including endothelial cells, lay adjacent to SIRT<\/jats:styled-content>1\u2010expressing cells in periapical granulomas. In addition, SIRT<\/jats:styled-content>1\u2010expressing cells were detected adjacent to VEGF<\/jats:styled-content>\u2010expressing cells and VEGF<\/jats:styled-content>\u2010 or VE<\/jats:styled-content>\u2010cadherin\u2010expressing endothelial cells. SIRT<\/jats:styled-content>1, VEGF<\/jats:styled-content> and VE<\/jats:styled-content>\u2010cadherin mRNA<\/jats:styled-content> expression levels in periapical granulomas were significantly higher (P\u00a0<\/jats:italic>=\u00a0<\/jats:italic>0.0054, 0.0090 and 0.0090, respectively) than those in healthy gingival tissues. HUVEC<\/jats:styled-content>s treated with resveratrol exhibited significantly higher expression of mRNA<\/jats:styled-content>s encoding SIRT<\/jats:styled-content>1, VEGF<\/jats:styled-content> and VE<\/jats:styled-content>\u2010cadherin (P\u00a0<\/jats:italic>=\u00a0<\/jats:italic>0.0019, 0.00005 and 0.0045, respectively) compared with controls, but sirtinol inhibited such expression. Resveratrol caused HUVEC<\/jats:styled-content>s to form tube\u2010like structures, whilst sirtinol inhibited this process.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>These findings suggest that SIRT<\/jats:styled-content>1 may stimulate angiogenesis in periapical granulomas by triggering the proliferation of endothelial cells and inducing VEGF<\/jats:styled-content> and VE<\/jats:styled-content>\u2010cadherin expression.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.12894","type":"journal-article","created":{"date-parts":[[2018,1,24]],"date-time":"2018-01-24T07:38:43Z","timestamp":1516779523000},"page":"747-757","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["A potential role for the silent information regulator 2 homologue 1 (SIRT<\/scp>1) in periapical periodontitis"],"prefix":"10.1111","volume":"51","author":[{"given":"H.","family":"Kudo","sequence":"first","affiliation":[{"name":"Department of Endodontics 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