{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T04:55:46Z","timestamp":1771649746708,"version":"3.50.1"},"reference-count":20,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2018,3,25]],"date-time":"2018-03-25T00:00:00Z","timestamp":1521936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","award":["2016YFC1000505"],"award-info":[{"award-number":["2016YFC1000505"]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81300870"],"award-info":[{"award-number":["81300870"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81470727"],"award-info":[{"award-number":["81470727"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To identify the role of astrocyte elevated gene\u20101 (<jats:styled-content style=\"fixed-case\">AEG<\/jats:styled-content>\u20101) and the mechanism underlying the inflammatory response in dental pulp cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>Astrocyte elevated gene\u20101 expression was detected at different stages of pulpitis in a rat model using immunohistochemistry. <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content> and Western blot were used to assess <jats:styled-content style=\"fixed-case\">AEG<\/jats:styled-content>\u20101 expression in human dental pulp cells stimulated by lipopolysaccharide (<jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>). The <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>\u2010induced expression of inflammatory cytokine genes was quantified by <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content> in cells transfected with <jats:styled-content style=\"fixed-case\">AEG<\/jats:styled-content>\u20101 or negative control (<jats:styled-content style=\"fixed-case\">NC<\/jats:styled-content>) si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>. Immunofluorescence and Western blot were utilized to evaluate the activation of <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010\u03baB signalling in <jats:styled-content style=\"fixed-case\">AEG<\/jats:styled-content>\u20101 knockdown cells. <jats:styled-content style=\"fixed-case\">AEG<\/jats:styled-content>\u20101 expression was also investigated by Western blot in dental pulp cells pre\u2010treated with inhibitors of <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010\u03baB and <jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content>\u20103K signalling before the addition of <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>. Data were analysed statistically using one\u2010way <jats:sc>anova<\/jats:sc>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The exposure of dental pulp tissue to <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content> resulted in acute inflammation with necrosis and <jats:styled-content style=\"fixed-case\">AEG<\/jats:styled-content>\u20101 expression in the pulp tissue beneath the perforation. In <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>\u2010stimulated dental pulp cells, <jats:styled-content style=\"fixed-case\">AEG<\/jats:styled-content>\u20101 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> and protein were significantly up\u2010regulated (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00 .05) in a time\u2010 and dose\u2010dependent manner. In <jats:styled-content style=\"fixed-case\">AEG<\/jats:styled-content>\u20101 knockdown cells, the synthesis of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106 and <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> was suppressed significantly (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) upon <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content> induction. <jats:styled-content style=\"fixed-case\">AEG<\/jats:styled-content>\u20101 knockdown also inhibited nuclear translocation of p65. Suppression of <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010\u03baB and <jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content>\u20103K abrogated the <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>\u2010induced up\u2010regulation of <jats:styled-content style=\"fixed-case\">AEG<\/jats:styled-content>\u20101.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Astrocyte elevated gene\u20101 participated in inflammatory cytokine synthesis via <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010\u03baB signalling in the dental pulp. Both <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010\u03baB and <jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content>\u20103K signalling are involved in <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>\u2010induced <jats:styled-content style=\"fixed-case\">AEG<\/jats:styled-content>\u20101 expression in dental pulp cells.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.12921","type":"journal-article","created":{"date-parts":[[2018,3,5]],"date-time":"2018-03-05T16:52:39Z","timestamp":1520268759000},"page":"1130-1138","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Astrocyte elevated gene\u20101 participates in the production of pro\u2010inflammatory cytokines in dental pulp cells via <scp>NF<\/scp>\u2010\u03baB signalling pathway"],"prefix":"10.1111","volume":"51","author":[{"given":"H.","family":"Wu","sequence":"first","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education School &amp; Hospital of Stomatology Wuhan University Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8255-8072","authenticated-orcid":false,"given":"M.","family":"He","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education School &amp; Hospital of Stomatology Wuhan University Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Yang","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education School &amp; Hospital of Stomatology Wuhan University Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Zuo","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education School &amp; Hospital of Stomatology Wuhan University Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.","family":"Bian","sequence":"additional","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education School &amp; Hospital of Stomatology Wuhan University Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,3,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-3029"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-401676-7.00003-6"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2423"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2007.01.001"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tice.2017.09.005"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2006.01.010"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910508400815"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2009.03063.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms7658"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0608386103"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.8.4414-4420.2003"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-09-3838"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M101103200"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2015.02.020"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-07-6164"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-09-1846"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034511405390"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.567644"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2009.137"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1155\/2010\/856087"}],"container-title":["International Endodontic Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fiej.12921","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iej.12921","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T23:51:15Z","timestamp":1694994675000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/iej.12921"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3,25]]},"references-count":20,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2018,10]]}},"alternative-id":["10.1111\/iej.12921"],"URL":"https:\/\/doi.org\/10.1111\/iej.12921","archive":["Portico"],"relation":{},"ISSN":["0143-2885","1365-2591"],"issn-type":[{"value":"0143-2885","type":"print"},{"value":"1365-2591","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,3,25]]},"assertion":[{"value":"2017-07-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-02-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-03-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}