{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T11:02:55Z","timestamp":1791889375479,"version":"4.3.4"},"reference-count":38,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2018,6,16]],"date-time":"2018-06-16T00:00:00Z","timestamp":1529107200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004410","name":"T\u00fcrkiye Bilimsel ve Teknolojik Ara\u015ftirma Kurumu","doi-asserted-by":"publisher","award":["214S569"],"award-info":[{"award-number":["214S569"]}],"id":[{"id":"10.13039\/501100004410","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To detect the unknown components of the oral microbiome and the effects of root canal treatment in a Turkish population and to evaluate the changes in microbial diversity in the root canals before and after treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>Single\u2010rooted central and lateral maxillary incisors with one canal were chosen from 20 patients. Baseline samples of intact intracanal microbiota were collected from 20 root canals of permanent teeth with necrotic pulps using sterile paper points. After root canal preparation, the root canals were filled with a calcium hydroxide paste for 7\u00a0days. Calcium hydroxide was removed from root canal with 2.5% sodium hypochlorite and 17% <jats:styled-content style=\"fixed-case\">EDTA<\/jats:styled-content> using passive ultrasonic irrigation (<jats:styled-content style=\"fixed-case\">PUI<\/jats:styled-content>). A second bacteriologic samples were taken with sterile paper points prior to root filling. The samples were processes with <jats:styled-content style=\"fixed-case\">DN<\/jats:styled-content>ase\u2010I treatment using next\u2010generation sequencing (<jats:styled-content style=\"fixed-case\">NGS<\/jats:styled-content>). Reduction in bacterial numbers during root canal treatment was evaluated using real\u2010time quantitative PCR (<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>). All statistical analyses were conducted using the <jats:styled-content style=\"fixed-case\">MINITAB<\/jats:styled-content> 17 software (Minitab Ltd. Co., Coventry, UK). A one\u2010sample <jats:italic>t<\/jats:italic>\u2010test was used to analyse the data. Statistical significance was accepted at <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Relative abundances of <jats:italic>Mycoplasma<\/jats:italic> sp., <jats:italic>Paludibacter<\/jats:italic> sp., <jats:italic>Tannerella<\/jats:italic> sp., <jats:italic>Prevotella<\/jats:italic> spp. and an uncultured species from the order <jats:italic>Bacteroidales<\/jats:italic> decreased with root canal preparation and medication (98.7%, 99.8%, 98.8%, 97.7% and 99.3%, respectively), whilst the relative abundances of <jats:italic>Methylobacterium<\/jats:italic> sp., <jats:italic>Corynebacterium<\/jats:italic> sp. and <jats:italic>Streptococcus infantis<\/jats:italic> increased (93.1%, 94.8% and 99.4%, respectively). Considerable numbers of <jats:italic>Streptophyta<\/jats:italic> species were detected before and after treatment. The ratio of <jats:italic>Agrobacterium<\/jats:italic> sp. in the treated teeth community and the ratio of order <jats:italic>Streptophyta<\/jats:italic> in the infected canals had negative correlations with the success of bacterial elimination.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The combination of <jats:styled-content style=\"fixed-case\">NGS<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content> techniques resulted in detection of previously unknown components of the oral microbiome and the effects of root canal treatment on their relative abundance in a Turkish population.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.12956","type":"journal-article","created":{"date-parts":[[2018,6,1]],"date-time":"2018-06-01T07:06:03Z","timestamp":1527836763000},"page":"1349-1357","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Detection of the 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