{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T07:57:25Z","timestamp":1788595045352,"version":"build-2803163510"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,7,3]],"date-time":"2018-07-03T00:00:00Z","timestamp":1530576000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["233025\/2014\u20106"],"award-info":[{"award-number":["233025\/2014\u20106"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2015\/09958\u20108"],"award-info":[{"award-number":["2015\/09958\u20108"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2019,1]]},"abstract":"Abstract<\/jats:title>Aim<\/jats:title>To investigate the DNA<\/jats:styled-content> methylation profiles of immune response\u2010related genes in apical periodontitis (AP<\/jats:styled-content>) lesions.<\/jats:p><\/jats:sec>Methodology<\/jats:title>The methylation profiles on the cytosine\u2010phosphate\u2010guanine (CpG) regions of 22 gene promoters involved in inflammation and autoimmunity were assessed in 60 human AP<\/jats:styled-content> lesions and 24 healthy periodontal ligaments (controls) using a pathway\u2010specific real\u2010time polymerase chain reaction array (EpiTect\u00ae Methyl Signature PCR<\/jats:styled-content> Array Human Inflammatory Response). Differentially methylated genes were subsequently assessed for their mRNA<\/jats:styled-content> expression. Data analyses (One\u2010way anova<\/jats:sc>, Tukey's multiple comparisons tests and Mann\u2013Whitney tests) were performed using GraphPad Prism 6 software. P<\/jats:italic> values\u00a0\u2264\u00a00.05 were considered statistically significant.<\/jats:p><\/jats:sec>Results<\/jats:title>Significant DNA<\/jats:styled-content> hypermethylation was observed for CXCL<\/jats:styled-content>3<\/jats:italic> and FADD<\/jats:styled-content><\/jats:italic> gene promoters in AP<\/jats:styled-content> lesions when compared to control tissues (P\u00a0<\/jats:italic><\u00a0<\/jats:italic>0.001) and among other genes (P\u00a0<\/jats:italic><\u00a0<\/jats:italic>0.05). In contrast, IL<\/jats:styled-content>12B<\/jats:italic> and IL<\/jats:styled-content>4R<\/jats:italic> were associated with significant hypomethylation in comparison to other genes (P\u00a0<\/jats:italic><\u00a0<\/jats:italic>0.05). IL<\/jats:styled-content>12B, IL<\/jats:styled-content>4R, CXCL<\/jats:styled-content>3<\/jats:italic> and FADD<\/jats:styled-content><\/jats:italic> had differential mRNA<\/jats:styled-content> expression in AP<\/jats:styled-content> lesions and controls (P\u00a0<\/jats:italic><\u00a0<\/jats:italic>0.001).<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Differential methylation profiles of immune response\u2010related genes, such as FADD<\/jats:styled-content><\/jats:italic>, CXCL<\/jats:styled-content>3<\/jats:italic>, IL<\/jats:styled-content>12B<\/jats:italic> and IL<\/jats:styled-content>4R,<\/jats:italic> may have an influence on individual AP<\/jats:styled-content> susceptibility and patient treatment outcomes, through their potential contributions to altered expression of disease\u2010relevant genes. Methylation and\/or genetic variations in additional genes may also contribute to the dynamics of AP<\/jats:styled-content> development and should be considered in future studies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.12966","type":"journal-article","created":{"date-parts":[[2018,6,15]],"date-time":"2018-06-15T06:31:33Z","timestamp":1529044293000},"page":"5-12","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["DNA<\/scp> methylation profiles of immune response\u2010related genes in apical periodontitis"],"prefix":"10.1111","volume":"52","author":[{"given":"C.","family":"Wichnieski","sequence":"first","affiliation":[{"name":"Department of Endodontics Pontifical Catholic University of Parana Curitiba Brazil"},{"name":"Department of Endodontics School of Dentistry at Houston University of Texas Health Science Center at Houston Houston TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Maheshwari","sequence":"additional","affiliation":[{"name":"Center for Craniofacial Research University of Texas School of Dentistry at Houston Houston TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7057-9850","authenticated-orcid":false,"given":"L. C.","family":"Souza","sequence":"additional","affiliation":[{"name":"Department of Endodontics School of Dentistry at Houston University of Texas Health Science Center at Houston Houston TX USA"},{"name":"Center for Craniofacial Research University of Texas School of Dentistry at Houston Houston TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Nieves","sequence":"additional","affiliation":[{"name":"Center for Craniofacial Research University of Texas School of Dentistry at Houston Houston TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4372-0158","authenticated-orcid":false,"given":"T.","family":"Tartari","sequence":"additional","affiliation":[{"name":"Department of Endodontics School of Dentistry at Houston University of Texas Health Science Center at Houston Houston TX USA"},{"name":"Department of Endodontics School of Dentistry of Bauru University of S\u00e3o Paulo (FOB\/USP) S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. P.","family":"Garlet","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences School of Dentistry of Bauru University of S\u00e3o Paulo (FOB\/USP) Bauru S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7883-5396","authenticated-orcid":false,"given":"E.","family":"Carneiro","sequence":"additional","affiliation":[{"name":"Department of Endodontics Pontifical Catholic University of Parana Curitiba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Letra","sequence":"additional","affiliation":[{"name":"Department of Endodontics School of Dentistry at Houston University of Texas Health Science Center at Houston Houston TX USA"},{"name":"Center for Craniofacial Research University of Texas School of Dentistry at Houston Houston TX USA"},{"name":"Department of Diagnostic and Biomedical Sciences School of Dentistry at Houston University of Texas Health Science Center at Houston Houston TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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