{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T18:02:27Z","timestamp":1787162547224,"version":"build-2736575974"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,12,8]],"date-time":"2018-12-08T00:00:00Z","timestamp":1544227200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To profile miRNA expression between inflamed and healthy human dental pulp tissues and to investigate how the upregulation of miR\u2010223\u20103p in the inflamed pulp tissue regulates odontoblast differentiation and regeneration.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methodology<\/jats:title>\n                    <jats:p>\n                      Microarray analysis was used to identify differences in miRNA expression patterns between healthy and inflamed pulp tissue. The results were validated using quantitative real\u2010time PCR. To determine the effect of miR\u2010223\u20103p on odontoblast differentiation, miR\u2010223\u20103p was overexpressed in human dental pulp stem cells (DPSCs), which were cultured in mineralizing induction medium (to induce odontoblast differentiation). To identify the target genes of miR\u2010223\u20103p, an SABiosciences Human Osteogenesis PCR Array, combined with bioinformatics, was used. Furthermore, a dual\u2010luciferase reporter assay and a small interfering RNA (siRNA) experiment were used to confirm the relationship between miR\u2010223\u20103p and its target gene. Statistical analysis was performed using the Student's\n                      <jats:italic>t<\/jats:italic>\n                      \u2010test or one\u2010way analysis of variance (\n                      <jats:sc>anova<\/jats:sc>\n                      );\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05 was considered statistically significant.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Seventy\u2010nine miRNAs were significantly differentially expressed (fold change &gt;2.0;\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05) between the two tissues. In particular, miR\u2010223\u20103p was markedly upregulated in inflamed dental pulp. Overexpression of miR\u2010223\u20103p in DPSCs significantly increased the protein levels of dentine sialophosphoprotein (DSPP) and dentine matrix protein 1 (DMP\u20101) (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05). However, the SMAD family member 3 (SMAD3) protein level was significantly lower than in control DPSCs (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05). Bioinformatics and the dual\u2010luciferase assay reporter assay indicated that Smad3 was a potential target of miR\u2010223\u20103p. Knockdown of Smad3 in DPSCs subjected to mineralization induction resulted in detection of DSPP and DMP\u20101 earlier than in control DPSCs, and it increased the protein level of alkaline phosphatase (ALP), thereby promoting odontoblast differentiation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>miR\u2010223\u20103p is implicated in the regulation of odontoblast differentiation, which may be involved in the process of pulpitis repair.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/iej.13032","type":"journal-article","created":{"date-parts":[[2018,10,28]],"date-time":"2018-10-28T03:03:57Z","timestamp":1540695837000},"page":"491-503","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Inflammation\u2010induced overexpression of microRNA\u2010223\u20103p regulates odontoblastic differentiation of human dental pulp stem cells by targeting SMAD3"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2976-1992","authenticated-orcid":false,"given":"X.","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Stomatology Guangzhou Women and Children's Medical Center Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Liu","sequence":"additional","affiliation":[{"name":"International Medical Center Guangdong Second Provincial General Hospital Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Hou","sequence":"additional","affiliation":[{"name":"Department of Stomatology Nanfang Hospital Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Stomatology Guangzhou Women and Children's 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