{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T02:49:49Z","timestamp":1769050189939,"version":"3.49.0"},"reference-count":44,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,12,11]],"date-time":"2018-12-11T00:00:00Z","timestamp":1544486400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To investigate pulp responses after pulpotomy and <jats:styled-content style=\"fixed-case\">EDTA<\/jats:styled-content> conditioning of pulp chamber dentinal walls with or without the placement of a collagenous scaffold in the experimental model of miniature swine teeth.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>Forty\u2010two fully developed permanent premolars and molars of healthy miniature swines were used. After preparation of pulp exposures through Class I cavities, the tissue of the pulp chamber was completely removed. The haemorrhage was controlled, and the root pulp was protected using a polyurethane film. The circumpulpal pulp chamber dentine was treated for 3\u00a0min with normal saline (group 1), or 17% <jats:styled-content style=\"fixed-case\">EDTA<\/jats:styled-content> solution (groups 2 and 3). The film was removed, and the pulp chamber cavities were left empty (groups 1 and 2), or filled with swine collagenous sponge (group 3). The access cavities were restored with a Teflon disc and glass ionomer. Teeth were evaluated histo\u2010morphologically after 10\u00a0weeks. Data were compared using the nonparametric Fisher's exact test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Teeth after treatment of dentine with saline (group 1) were associated with no or only traces of hard tissue formation along the root canal walls. Atubular tertiary dentine deposition in the form of matrix deposition along root canal walls, or dentine bridge formation at the orifice of root canals or complete pulp canal obliteration, was found after treatment of dentine with <jats:styled-content style=\"fixed-case\">EDTA<\/jats:styled-content> in both experiments (groups 2 and 3). Significantly different types of mineralization in the root canals of groups 2 and 3 were seen (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.001). Tissue changes in the pulp cavity, characterized by soft tissue growth and osteodentine or atubular tertiary dentine formation, were only seen after <jats:styled-content style=\"fixed-case\">EDTA<\/jats:styled-content> conditioning of dentine, in 6.2% of the teeth without scaffold and 64.7% of the teeth with scaffold application. Newly deposited mineralized matrix in the pulp chamber was always in continuation with hard tissue deposited in the root canals.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The <jats:styled-content style=\"fixed-case\">EDTA<\/jats:styled-content> conditioning of pulp cavity dentinal walls after pulpotomy induced dentinogenic events in the root pulp. Application of collagenous scaffold in the pulp chamber enhanced soft tissue growth and mineralized tissue formation along the treated circumpulpal dentine.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.13048","type":"journal-article","created":{"date-parts":[[2018,11,26]],"date-time":"2018-11-26T08:30:29Z","timestamp":1543221029000},"page":"656-664","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["EDTA conditioning of circumpulpal dentine induces dentinogenic events in pulpotomized miniature swine teeth"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9602-9091","authenticated-orcid":false,"given":"D.","family":"Tziafas","sequence":"first","affiliation":[{"name":"Hamdan Bin Mohammed College of Dental Medicine Mohammed Bin Rashid University of Medicine and Health Sciences Dubai United Arab Emirates"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Kodonas","sequence":"additional","affiliation":[{"name":"School of Dentistry Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2790-4264","authenticated-orcid":false,"given":"C.","family":"Gogos","sequence":"additional","affiliation":[{"name":"School of Dentistry Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Tziafa","sequence":"additional","affiliation":[{"name":"School of Dentistry Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Papadimitriou","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine Aristotle University of Thessaloniki Thessaloniki Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,12,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdent.2015.12.005"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2013.11.008"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2014.11.015"},{"key":"e_1_2_7_5_1","first-page":"67","volume-title":"Monographs in Oral Science","author":"Baume LJ","year":"1980"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/08959374010150012201"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tripleo.2005.03.032"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9969(96)00115-X"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/iej.12627"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-3913(87)90104-1"},{"key":"e_1_2_7_11_1","first-page":"4","article-title":"Tunnel defects in dentin bridges: their formation following direct pulp capping","volume":"21","author":"Cox CF","year":"1996","journal-title":"Operative Dentistry"},{"key":"e_1_2_7_12_1","first-page":"407","article-title":"Dentin matrix component solubilization by solutions of pH relevant to self\u2010etching dental adhesives","volume":"15","author":"Ferracane JL","year":"2013","journal-title":"Journal of Adhesive Dentistry"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650050708"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2014.11.021"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/iej.12492"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2005.12.020"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-9657.1985.tb00555.x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.2217\/rme.09.5"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0030-4220(65)90166-0"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1177\/039463200902200315"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-9657.1993.tb00279.x"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1177\/08959374010150010201"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034511405323"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2013.01.005"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2013.09.007"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdent.2014.06.012"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9969(00)00075-3"},{"key":"e_1_2_7_28_1","first-page":"185","volume-title":"Tissue Engineering. 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