{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T04:49:25Z","timestamp":1781758165287,"version":"3.54.5"},"reference-count":19,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,1,20]],"date-time":"2019-01-20T00:00:00Z","timestamp":1547942400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100010120","name":"Huashan Hospital","doi-asserted-by":"publisher","award":["8157030373"],"award-info":[{"award-number":["8157030373"]}],"id":[{"id":"10.13039\/501100010120","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81570965"],"award-info":[{"award-number":["81570965"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To identify the basic characteristics and gene expression profiles of supernumerary teeth derived stem cells (<jats:styled-content style=\"fixed-case\">SNTSC<\/jats:styled-content>s) and compare them with those of normal dental pulp stem cells (<jats:styled-content style=\"fixed-case\">DPSC<\/jats:styled-content>s).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>Flow cytometry was conducted to identify the protein expression of stem cell markers. Cell proliferation, migration and differentiation abilities of both <jats:styled-content style=\"fixed-case\">SNTSC<\/jats:styled-content>s and <jats:styled-content style=\"fixed-case\">DPSC<\/jats:styled-content>s were determined by <jats:styled-content style=\"fixed-case\">CCK<\/jats:styled-content>8, transwell and differentiation assays, respectively. Gene expression profiles were studied by <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> sequencing analyses. After knocking down the expression of certain differential expression genes (<jats:styled-content style=\"fixed-case\">DEG<\/jats:styled-content>s), the function of <jats:styled-content style=\"fixed-case\">DEG<\/jats:styled-content>s was investigated by <jats:styled-content style=\"fixed-case\">CCK<\/jats:styled-content>8 and transwell assays. Statistical differences were determined using a two\u2010tailed <jats:italic>t<\/jats:italic>\u2010test and <jats:italic>P<\/jats:italic> values below 0.05 were considered significant.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Supernumerary teeth derived stem cells were capable of differentiating into adipocyte, chondrocyte and osteoblast lineage cells, and compared to ordinary <jats:styled-content style=\"fixed-case\">DPSC<\/jats:styled-content>s, <jats:styled-content style=\"fixed-case\">SNTSC<\/jats:styled-content>s had a significantly higher cell proliferation rate (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01) and significantly lower migration rate (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01). <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010seq results revealed the differential expression genes (<jats:styled-content style=\"fixed-case\">DEG<\/jats:styled-content>s) between <jats:styled-content style=\"fixed-case\">SNTSC<\/jats:styled-content>s and <jats:styled-content style=\"fixed-case\">DPSC<\/jats:styled-content>s. A principal component analysis (<jats:styled-content style=\"fixed-case\">PCA<\/jats:styled-content>) and cluster analysis revealed that the gene expression patterns of <jats:styled-content style=\"fixed-case\">SNTSC<\/jats:styled-content>s and <jats:styled-content style=\"fixed-case\">DPSC<\/jats:styled-content>s were different from each other. A total of 12\u00a0861 genes were differentially expressed at a significant <jats:italic>P<\/jats:italic> value (<jats:italic>P<\/jats:italic>\u00a0\u2264\u00a00.01), and 5292 of these increased in <jats:styled-content style=\"fixed-case\">SNTSC<\/jats:styled-content>s and 7569 decreased. Further study on the selected <jats:styled-content style=\"fixed-case\">DEG<\/jats:styled-content>s revealed that <jats:styled-content style=\"fixed-case\">FUT<\/jats:styled-content>11, <jats:styled-content style=\"fixed-case\">FAM<\/jats:styled-content>155A and <jats:styled-content style=\"fixed-case\">BRD<\/jats:styled-content>2 inhibited the cell proliferation rate of <jats:styled-content style=\"fixed-case\">SNTSC<\/jats:styled-content>s, and <jats:styled-content style=\"fixed-case\">FUT<\/jats:styled-content>11 and <jats:styled-content style=\"fixed-case\">GLUD<\/jats:styled-content>1 inhibited the cell migration rate, whilst <jats:styled-content style=\"fixed-case\">FAM<\/jats:styled-content>155A promoted the migration rate.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The biological characteristics and gene expression profile of <jats:styled-content style=\"fixed-case\">SNTSC<\/jats:styled-content>s was revealed. The stem cell properties of <jats:styled-content style=\"fixed-case\">SNTSC<\/jats:styled-content>s were similar to normal <jats:styled-content style=\"fixed-case\">DPSC<\/jats:styled-content>s but they had a high cell proliferation rate and may have greater potential for cell differentiation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.13060","type":"journal-article","created":{"date-parts":[[2018,12,19]],"date-time":"2018-12-19T12:02:38Z","timestamp":1545220958000},"page":"819-828","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["A biological study of supernumerary teeth derived dental pulp stem cells based on <scp>RNA<\/scp>\u2010seq analysis"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1629-9521","authenticated-orcid":false,"given":"X.","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Stomatology Huashan Hospital Fudan University Shanghai 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