{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T22:08:30Z","timestamp":1761948510891,"version":"3.40.5"},"reference-count":49,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,5,20]],"date-time":"2019-05-20T00:00:00Z","timestamp":1558310400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["PDSE20161618381"],"award-info":[{"award-number":["PDSE20161618381"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2019,9]]},"abstract":"Abstract<\/jats:title>Aim<\/jats:title>To investigate the expression of TP<\/jats:styled-content>63 in apical periodontitis (AP<\/jats:styled-content>) tissues and the association of single nucleotide polymorphisms (SNP<\/jats:styled-content>s) in the TP<\/jats:styled-content>63<\/jats:italic> gene with AP<\/jats:styled-content> using a case\u2010control dataset.<\/jats:p><\/jats:sec>Methodology<\/jats:title>Expression of TP<\/jats:styled-content>63 in human AP<\/jats:styled-content> lesions (apical abscess, radicular cyst, periapical granuloma) was evaluated using immunohistochemistry. A case\u2010control association study was performed to assess the association of TP<\/jats:styled-content>63<\/jats:italic> polymorphisms in individuals having AP<\/jats:styled-content> with or without associated pain. Cases were defined as subjects with deep caries and AP<\/jats:styled-content> (n<\/jats:italic>\u00a0=\u00a0151) and subjects with symptomatic apical periodontitis or acute apical abscess (n<\/jats:italic>\u00a0=\u00a0124). Subjects without AP<\/jats:styled-content> (n<\/jats:italic>\u00a0=\u00a0169) and asymptomatic (n<\/jats:italic>\u00a0=\u00a0196) were used as controls, respectively. Saliva samples were collected as source of genomic DNA<\/jats:styled-content>. Twelve SNP<\/jats:styled-content>s in the TP<\/jats:styled-content>63<\/jats:italic> gene were selected for genotyping using Taqman chemistry in real\u2010time PCR<\/jats:styled-content>. Data analysis was performed using PLINK<\/jats:styled-content> software. The Bonferroni method was applied to correct for multiple testing; \u03b1\u00a0\u2264\u00a00.004 indicates significant differences between groups.<\/jats:p><\/jats:sec>Results<\/jats:title>TP<\/jats:styled-content>63 expression was evident in apical abscesses and radicular cysts, while weaker expression was observed in periapical granulomas. Positive expression was observed in mononuclear cells in the granulation tissues of all AP<\/jats:styled-content> lesions. Regarding the presence of AP<\/jats:styled-content>, a trend for allelic association was observed for rs16864812 and rs9810322 (P<\/jats:italic>\u00a0=\u00a00.04) and rs9810322 genotypes were also nominally associated with AP<\/jats:styled-content> under a dominant model (P<\/jats:italic>\u00a0=\u00a00.04). When considering the presence of periapical pain, a trend for allelic and genotypic association was observed for rs10155037 (P<\/jats:italic>\u00a0=\u00a00.03). Haplotypes were also associated with AP<\/jats:styled-content> and periapical pain (P<\/jats:italic>\u00a0\u2264\u00a00.05).<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Apical periodontitis is a complex multifactorial condition and it is likely that multiple genes and environmental effects may influence its susceptibility, progression or both. TP<\/jats:styled-content>63 variants may play a role in AP<\/jats:styled-content> pathogenesis and susceptibility, individually or interactively with other genes. Additional studies in other populations and functional studies are needed to improve understanding of the role of TP<\/jats:styled-content>63<\/jats:italic> in AP<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.13133","type":"journal-article","created":{"date-parts":[[2019,4,26]],"date-time":"2019-04-26T08:22:17Z","timestamp":1556266937000},"page":"1344-1353","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Potential role of TP<\/scp>63 in apical periodontitis development"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7057-9850","authenticated-orcid":false,"given":"L. C.","family":"Souza","sequence":"first","affiliation":[{"name":"Department of Endodontics School of Dentistry at Houston University of Texas Health Science Center at Houston Houston"},{"name":"Center for Craniofacial Research University of Texas School of Dentistry at Houston Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. M.","family":"Crozeta","sequence":"additional","affiliation":[{"name":"Department of Endodontics School of Dentistry at Houston University of Texas Health Science Center at Houston Houston"},{"name":"Department of Endodontics University of S\u00e3o Paulo Ribeir\u00e3o Preto Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Guajardo","sequence":"additional","affiliation":[{"name":"Department of Endodontics School of Dentistry at Houston University of Texas Health Science Center at Houston Houston"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. H.","family":"Brasil da Costa","sequence":"additional","affiliation":[{"name":"Department of Biosciences Rice University Houston"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7696-7600","authenticated-orcid":false,"given":"M. D.","family":"Sousa\u2010Neto","sequence":"additional","affiliation":[{"name":"Department of Endodontics University of S\u00e3o Paulo Ribeir\u00e3o Preto Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Letra","sequence":"additional","affiliation":[{"name":"Department of Endodontics School of Dentistry at Houston University of Texas Health Science Center at Houston Houston"},{"name":"Center for Craniofacial Research University of Texas School of Dentistry at Houston Houston TX USA"},{"name":"Department of Diagnostic and Biomedical Sciences School of Dentistry at Houston University of Texas Health Science Center at Houston Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. M.","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Endodontics School of Dentistry at Houston University of Texas Health Science Center at Houston Houston"},{"name":"Center for Craniofacial Research University of Texas School of Dentistry at Houston Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,5,20]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_8_2_1","DOI":"10.1111\/j.1365-2591.2012.02097.x"},{"doi-asserted-by":"publisher","key":"e_1_2_8_3_1","DOI":"10.1016\/j.jid.2016.01.013"},{"doi-asserted-by":"publisher","key":"e_1_2_8_4_1","DOI":"10.1155\/2013\/624176"},{"doi-asserted-by":"publisher","key":"e_1_2_8_5_1","DOI":"10.1002\/humu.22523"},{"doi-asserted-by":"publisher","key":"e_1_2_8_6_1","DOI":"10.1086\/381000"},{"doi-asserted-by":"publisher","key":"e_1_2_8_7_1","DOI":"10.1038\/s10038-018-0423-1"},{"doi-asserted-by":"publisher","key":"e_1_2_8_8_1","DOI":"10.1073\/pnas.1620664114"},{"doi-asserted-by":"publisher","key":"e_1_2_8_9_1","DOI":"10.1007\/s00018-017-2666-y"},{"doi-asserted-by":"publisher","key":"e_1_2_8_10_1","DOI":"10.1155\/2016\/4348208"},{"doi-asserted-by":"publisher","key":"e_1_2_8_11_1","DOI":"10.18632\/oncotarget.22372"},{"doi-asserted-by":"publisher","key":"e_1_2_8_12_1","DOI":"10.1158\/1055-9965.EPI-08-0785"},{"key":"e_1_2_8_13_1","first-page":"494","article-title":"p63 expression profiles in human normal and tumor tissues","volume":"8","author":"Como CJ","year":"2002","journal-title":"Clinical Cancer Research"},{"doi-asserted-by":"publisher","key":"e_1_2_8_14_1","DOI":"10.1038\/modpathol.2008.29"},{"doi-asserted-by":"publisher","key":"e_1_2_8_15_1","DOI":"10.1016\/j.joen.2014.10.016"},{"doi-asserted-by":"publisher","key":"e_1_2_8_16_1","DOI":"10.1515\/prilozi-2015-0070"},{"doi-asserted-by":"publisher","key":"e_1_2_8_17_1","DOI":"10.1590\/S0103-64402012000400005"},{"doi-asserted-by":"publisher","key":"e_1_2_8_18_1","DOI":"10.1186\/1746-1596-7-130"},{"doi-asserted-by":"publisher","key":"e_1_2_8_19_1","DOI":"10.1097\/01.pai.0000142160.52670.ce"},{"doi-asserted-by":"publisher","key":"e_1_2_8_20_1","DOI":"10.1007\/s13277-013-1248-3"},{"doi-asserted-by":"publisher","key":"e_1_2_8_21_1","DOI":"10.1002\/ijc.23173"},{"doi-asserted-by":"publisher","key":"e_1_2_8_22_1","DOI":"10.1615\/CritRevImmunol.v32.i1.30"},{"doi-asserted-by":"publisher","key":"e_1_2_8_23_1","DOI":"10.1016\/j.cell.2011.10.001"},{"doi-asserted-by":"publisher","key":"e_1_2_8_24_1","DOI":"10.3892\/ijo.2012.1727"},{"doi-asserted-by":"publisher","key":"e_1_2_8_25_1","DOI":"10.1038\/modpathol.3801023"},{"key":"e_1_2_8_26_1","first-page":"175721","article-title":"Regulation of p63 protein stability via ubiquitin\u2010proteasome pathway","volume":"2014","author":"Li C","year":"2014","journal-title":"Biomed Research International"},{"doi-asserted-by":"publisher","key":"e_1_2_8_27_1","DOI":"10.1016\/j.ijom.2004.12.002"},{"doi-asserted-by":"publisher","key":"e_1_2_8_28_1","DOI":"10.1016\/j.joen.2016.07.010"},{"doi-asserted-by":"publisher","key":"e_1_2_8_29_1","DOI":"10.1084\/jem.20121439"},{"doi-asserted-by":"publisher","key":"e_1_2_8_30_1","DOI":"10.1016\/j.joen.2012.02.009"},{"doi-asserted-by":"publisher","key":"e_1_2_8_31_1","DOI":"10.1016\/j.joen.2011.01.009"},{"doi-asserted-by":"publisher","key":"e_1_2_8_32_1","DOI":"10.1111\/j.1365-2559.2006.02507.x"},{"doi-asserted-by":"publisher","key":"e_1_2_8_33_1","DOI":"10.3346\/jkms.2005.20.5.752"},{"doi-asserted-by":"publisher","key":"e_1_2_8_34_1","DOI":"10.1186\/1471-2350-14-103"},{"doi-asserted-by":"publisher","key":"e_1_2_8_35_1","DOI":"10.1086\/519795"},{"doi-asserted-by":"publisher","key":"e_1_2_8_36_1","DOI":"10.1111\/j.1601-1546.2004.00097.x"},{"volume-title":"Endodontology: An integrated biological and clinical view","year":"2013","author":"Ricucci D","key":"e_1_2_8_37_1"},{"doi-asserted-by":"publisher","key":"e_1_2_8_38_1","DOI":"10.1038\/cdd.2015.162"},{"doi-asserted-by":"publisher","key":"e_1_2_8_39_1","DOI":"10.1016\/j.joen.2013.10.006"},{"key":"e_1_2_8_40_1","doi-asserted-by":"crossref","first-page":"776","DOI":"10.5858\/2010-0291-OA.1","article-title":"p63 expression in giant cell\u2010containing lesions of bone and soft tissue","volume":"135","author":"Roza G","year":"2011","journal-title":"Archives of Pathology & Laboratory Medicine"},{"doi-asserted-by":"publisher","key":"e_1_2_8_41_1","DOI":"10.1007\/s100380200086"},{"doi-asserted-by":"publisher","key":"e_1_2_8_42_1","DOI":"10.1016\/j.prp.2016.07.007"},{"doi-asserted-by":"publisher","key":"e_1_2_8_43_1","DOI":"10.1590\/S0103-64402007000400001"},{"doi-asserted-by":"publisher","key":"e_1_2_8_44_1","DOI":"10.1007\/s00018-017-2701-z"},{"doi-asserted-by":"publisher","key":"e_1_2_8_45_1","DOI":"10.1590\/1678-775720160112"},{"doi-asserted-by":"publisher","key":"e_1_2_8_46_1","DOI":"10.1097\/GIM.0b013e3181833793"},{"doi-asserted-by":"publisher","key":"e_1_2_8_47_1","DOI":"10.1002\/ijc.29978"},{"doi-asserted-by":"publisher","key":"e_1_2_8_48_1","DOI":"10.1038\/19539"},{"doi-asserted-by":"publisher","key":"e_1_2_8_49_1","DOI":"10.1016\/j.gene.2013.11.038"},{"doi-asserted-by":"publisher","key":"e_1_2_8_50_1","DOI":"10.1165\/rcmb.2013-0327OC"}],"container-title":["International Endodontic Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iej.13133","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/iej.13133","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iej.13133","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T06:13:13Z","timestamp":1694326393000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/iej.13133"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,5,20]]},"references-count":49,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2019,9]]}},"alternative-id":["10.1111\/iej.13133"],"URL":"https:\/\/doi.org\/10.1111\/iej.13133","archive":["Portico"],"relation":{},"ISSN":["0143-2885","1365-2591"],"issn-type":[{"type":"print","value":"0143-2885"},{"type":"electronic","value":"1365-2591"}],"subject":[],"published":{"date-parts":[[2019,5,20]]},"assertion":[{"value":"2018-11-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-05-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}