{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:51:50Z","timestamp":1787014310979,"version":"build-2736575974"},"reference-count":47,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,7,26]],"date-time":"2019-07-26T00:00:00Z","timestamp":1564099200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To investigate the role of GATA\u2010binding protein 4 (GATA4) in the inflammatory response induced by DNA double\u2010strand breaks (DSBs) in human dental pulp cells (hDPCs).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methodology<\/jats:title>\n                    <jats:p>\n                      Lipopolysaccharide (LPS) was used for stimulating inflammation in dental pulp tissue\n                      <jats:italic>in vivo<\/jats:italic>\n                      and hDPCs\n                      <jats:italic>in vitro<\/jats:italic>\n                      . Expression levels of GATA4 and \u03b3\u2010H2A.X (a marker for DSBs) were detected at different stages of pulpitis in a rat model and human pulp tissues by immunohistochemistry. Real\u2010time quantitative polymerase chain reaction and Western blot were performed to assess expression of GATA4 and \u03b3\u2010H2A.X and the activation of nuclear factor \u03baB (NF\u2010\u03baB) in hDPCs stimulated by LPS. The comet assay was used for detecting the extent of DSBs in hDPCs. Immunocytochemistry and Western blot were utilized to evaluate expression of \u03b3\u2010H2A.X and GATA4 and activation of NF\u2010\u03baB in hDPCs pre\u2010treated with inhibitors of DNA damage response or transfected with GATA4 small interfering RNA before the treatment of LPS. Data were analysed statistically using one\u2010way\n                      <jats:sc>anova<\/jats:sc>\n                      or Kruskal\u2013Wallis tests.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The expression of GATA4 and activation of DNA damage response and NF\u2010\u03baB in inflamed pulp tissue and LPS\u2010treated hDPCs were identified. Significantly decreased expression of GATA4 and significantly decreased inflammatory processes in hDPCs were demonstrated via suppression of DNA damage response (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05). In GATA4\u2010knockdown cells, the expression of \u03b3\u2010H2A.X did not change, but nuclear translocation of p65 was significantly suppressed (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05) upon induction by LPS.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Lipopolysaccharide\u2010induced DSBs activated the NF\u2010\u03baB signalling pathway in hDPCs, and GATA4 acts as a positive moderator of the progress. The involvement of GATA4 in this pathology may serve as a therapeutic target in pulpitis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/iej.13180","type":"journal-article","created":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T15:08:51Z","timestamp":1561993731000},"page":"1704-1715","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Lipopolysaccharide\u2010induced DNA damage response activates nuclear factor \u03baB signalling pathway via GATA4 in dental pulp cells"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8816-9198","authenticated-orcid":false,"given":"W.","family":"Qiao","sequence":"first","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology Wuhan University Wuhan Hubei 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