{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T05:11:36Z","timestamp":1777439496393,"version":"3.51.4"},"reference-count":17,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,8,25]],"date-time":"2019-08-25T00:00:00Z","timestamp":1566691200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004263","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Rio Grande do Sul","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004263","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["443699\/2014\u20103"],"award-info":[{"award-number":["443699\/2014\u20103"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To describe an <jats:italic>in vitro<\/jats:italic> experimental model of cystic structure formation to conduct research on radicular cyst development.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>To form spheroid structures, various numbers (1\u00a0\u00d7\u00a010<jats:sup>4<\/jats:sup>, 5\u00a0\u00d7\u00a010<jats:sup>4<\/jats:sup> or 1\u00a0\u00d7\u00a010<jats:sup>5<\/jats:sup>) of epithelial cells (HaCaT and Cal27) were seeded in 96\u2010well plates previously coated with 1.5% low\u2010melting agarose. After 24\u00a0h, the spheroids were collected, embedded in 3D collagen matrix and transferred to 24\u2010well plates previously coated with polymerized collagen and kept for up to 21\u00a0days. Images of spheroids were captured at each time\u2010point (1, 5, 9, 15 and 21\u00a0days), and samples underwent histological and confocal microscopy analyses. Spheroid area, perimeter and cell dispersion were measured. One\u2010way Anova was used for statistical analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Both epithelial cell lines were able to generate regular and circular spheroids after 24\u00a0h of incubation regardless of cell density. Spheroid structures in the collagen matrix were uniform in most samples until day 15, when several spots that appeared to be new cultures were seen. Spheroids from HaCaT were significantly more stable than those from Cal27 (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.05). Starting on the third day, the examination of histological sections revealed a cavity with epithelial lining morphology, similar to a pathological radicular cyst.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study describes an experimental model of cystogenesis <jats:italic>in vitro<\/jats:italic> that may be used to test theories and investigates the effects of different growth factors during cyst development and maintenance.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.13193","type":"journal-article","created":{"date-parts":[[2019,7,29]],"date-time":"2019-07-29T16:19:05Z","timestamp":1564417145000},"page":"1750-1757","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Development of an <i>in vitro<\/i> model to study tooth cystogenesis"],"prefix":"10.1111","volume":"52","author":[{"given":"N. K.","family":"Laureano","sequence":"first","affiliation":[{"name":"Basic Research Center, Dentistry School Federal University of Rio Grande do Sul Porto Alegre Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9756-4053","authenticated-orcid":false,"given":"L.","family":"Bernardi","sequence":"additional","affiliation":[{"name":"Basic Research Center, Dentistry School Federal University of Rio Grande do Sul Porto Alegre Brazil"},{"name":"Department of Morphological Sciences Federal University of Rio Grande do Sul Porto Alegre Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Bundrich","sequence":"additional","affiliation":[{"name":"Basic Research Center, Dentistry School Federal University of Rio Grande do Sul Porto Alegre Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. M.","family":"Brand","sequence":"additional","affiliation":[{"name":"Basic Research Center, Dentistry School Federal University of Rio Grande do Sul Porto Alegre Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Visioli","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology, Dentistry School Federal University of Rio Grande do Sul Porto Alegre Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. L.","family":"Lamers","sequence":"additional","affiliation":[{"name":"Department of Morphological Sciences Federal University of Rio Grande do Sul Porto Alegre Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. 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