{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T23:01:20Z","timestamp":1789513280791,"version":"build-2803163510"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,9,2]],"date-time":"2019-09-02T00:00:00Z","timestamp":1567382400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To verify whether experimentally induced pulpitis activates the expression of transient receptor potential vanilloid type 1 (TRPV1) and c\u2010Fos, both peripherally and centrally.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>Acute pulpitis was induced in Sprague\u2010Dawley rats via pulp exposure and application of complete Freund's adjuvant (CFA; <jats:italic>n<\/jats:italic>\u00a0=\u00a013). Saline\u2010treated (<jats:italic>n<\/jats:italic>\u00a0=\u00a013) rats and rats that did not undergo tooth preparation (<jats:italic>n<\/jats:italic>\u00a0=\u00a013) served as control groups. Three days post\u2010CFA or post\u2010saline application, face grooming activity was recorded, and the rats were then euthanized to allow for immunohistochemical analysis of the trigeminal ganglion (TG) and spinal trigeminal nucleus. <jats:sc>anova<\/jats:sc> with Student's <jats:italic>t<\/jats:italic>\u2010test for <jats:italic>post\u2010hoc<\/jats:italic> analysis was used to quantify the differences in behavioural tests and immunohistochemical labelling (c\u2010Fos and TRPV1) in TG amongst groups. Kruskal\u2013Wallis test with Dunnett's test for <jats:italic>post\u2010hoc<\/jats:italic> analysis was used to compare immunohistochemical labelling (c\u2010Fos and TRPV1) in the brainstem amongst groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Histological evidence of severe pulp inflammation was found, and there was a significant increase in pain\u2010like behaviour (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) in CFA\u2010treated animals. C\u2010Fos labelling and TRPV1 immunoreactivity in the TG were significantly higher (both <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) in the CFA group than in the control groups. In the spinal trigeminal nucleus, the immunoreactivity for c\u2010Fos was absent in the intermediate region (trigeminal subnucleus interpolaris) in all animals, with comparable expression of TRPV1 amongst all groups. In contrast, neurons in the trigeminal subnucleus caudalis (TSC) exhibited significant c\u2010Fos immunoreactivity in the CFA group (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.0063). The expression of TRPV1 did not differ amongst the three groups, but the superficial laminae of the TSC exhibited significantly greater expression of TRPV1 than did the deep layers (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.0014).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Following acute pulp inflammation, the TRPV1 channel was significantly involved in nociceptive signal processing in the peripheral nervous system, but not in the CNS. Because pulpitis induced some neuronal activation at the brainstem levels, further studies are needed to identify additional transducers that mediate signal transmission from pulpal afferents to their central targets.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.13204","type":"journal-article","created":{"date-parts":[[2019,8,14]],"date-time":"2019-08-14T13:17:56Z","timestamp":1565788676000},"page":"62-71","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Role of transient receptor potential vanilloid type 1 in the trigeminal ganglion and brain stem following dental pulp inflammation"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7993-672X","authenticated-orcid":false,"given":"M.","family":"Cha","sequence":"first","affiliation":[{"name":"Department of Physiology Oral Science Research Center Yonsei University College of Dentistry Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Sallem","sequence":"additional","affiliation":[{"name":"Department of Conservative Dentistry and Oral Science Research Center Yonsei University College of Dentistry Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. W.","family":"Jang","sequence":"additional","affiliation":[{"name":"Department of Conservative Dentistry and Oral Science Research Center Yonsei University College of Dentistry Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8972-2664","authenticated-orcid":false,"given":"I. Y.","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Conservative Dentistry and Oral Science Research Center Yonsei University College of Dentistry Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,9,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(02)03972-0"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2017.06.004"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(00)01936-3"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/39807"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.288.5464.306"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3959(02)00054-4"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/WNR.0000000000000193"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1998.80.5.2621"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034511413284"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1246-06.2006"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/0333102411398152"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vascn.2015.09.006"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2009.03.027"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0705546"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0207411"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12031-012-9849-7"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0361-9230(97)00277-3"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/papr.12034"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1984.51.5.890"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchemneu.2014.06.006"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2015.09.011"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(00)03253-4"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(02)00908-X"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresbull.2014.04.015"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2013.12.011"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902230406"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2014.05.034"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0052840"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2009.12.035"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2007.01.023"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2012.04.027"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1177\/10454411000110010401"},{"key":"e_1_2_8_34_1","first-page":"117","article-title":"Peripheral and central mechanisms of orofacial pain and their clinical correlates","volume":"71","author":"Sessle BJ","year":"2005","journal-title":"Minerva Anestesiologica"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2010.03.002"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034514565645"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3959(98)00039-6"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.2334\/josnusd.16-0854"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034510363231"}],"container-title":["International Endodontic Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iej.13204","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/iej.13204","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iej.13204","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T14:12:14Z","timestamp":1694182334000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/iej.13204"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,2]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1]]}},"alternative-id":["10.1111\/iej.13204"],"URL":"https:\/\/doi.org\/10.1111\/iej.13204","archive":["Portico"],"relation":{},"ISSN":["0143-2885","1365-2591"],"issn-type":[{"value":"0143-2885","type":"print"},{"value":"1365-2591","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9,2]]},"assertion":[{"value":"2019-03-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-09-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}