{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T09:00:30Z","timestamp":1772787630616,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,9,18]],"date-time":"2019-09-18T00:00:00Z","timestamp":1568764800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81970930"],"award-info":[{"award-number":["81970930"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81670986"],"award-info":[{"award-number":["81670986"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81800957"],"award-info":[{"award-number":["81800957"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To comparatively evaluate changes in\u00a0the proliferation and mineralization abilities of dental pulp stem cells (DPSCs) from juvenile and adult rats in a lipopolysaccharide (LPS)\u2010induced inflammatory microenvironment to provide a theoretical basis for the age\u2010related differences observed in DPSCs during repair of inflammatory injuries.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>DPSCs were isolated from juvenile (JDPSCs) and adult rats (ADPSCs), and senescence\u2010associated \u03b2\u2010galactosidase staining was used to compare senescence between JDPSCs and ADPSCs. Effects of LPS on JDPSCs and ADPSCs proliferation were investigated by cell counting kit\u20108 assays and flow cytometry. Alizarin red staining, quantitative reverse transcription polymerase chain reaction and Western blot assay were used to examine the effects of LPS on mineralization\u2010related genes and proteins in JDPSCs and ADPSCs. Immunohistochemistry was used to compare interleukin\u20101\u03b2 (IL\u20101\u03b2) and osteocalcin (OCN) expression in the pulpitis model. Unpaired Student's <jats:italic>t<\/jats:italic>\u2010tests and one\u2010way <jats:sc>anova<\/jats:sc> were used for statistical analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>DPSCs were isolated from juvenile and adult rat dental pulp tissues. At low concentrations (0.1\u20131\u00a0\u03bcg\u00a0mL<jats:sup>\u22121<\/jats:sup>), LPS significantly promoted the proliferation of JDPSCs (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.01) and ADPSCs (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.01 or <jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.05), with the effect being stronger in JDPSCs than in ADPSCs. In addition, mineralized nodules and the expression of mineralization\u2010related genes (<jats:italic>OCN<\/jats:italic>,<jats:italic> DSPP<\/jats:italic>,<jats:italic> ALP<\/jats:italic>,<jats:italic> BSP<\/jats:italic>) increased significantly after stimulation with LPS (0.5\u00a0\u03bcg\u00a0mL<jats:sup>\u22121<\/jats:sup>) in JDPSCs and ADPSCs (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.01 or <jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.05), and JDPSCs displayed a more obvious increase than ADPSCs. Western blots revealed OCN and ALP expression levels in JDPSCs treated with LPS were significantly upregulated (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.05); meanwhile, ALP expression in ADPSCs increased slightly but significantly (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.05), and OCN expression was not affected. Finally, IL\u20101\u03b2 expression was significantly higher (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.05) and OCN expression was significantly lower (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.05) in the inflamed dental pulp of adult rats than in juvenile rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>A certain degree of inflammatory stimulation promoted the proliferation and mineralization of DPSCs; however, this effect declined with age. The DPSCs of adult donors in an inflammatory microenvironment have a weaker repair ability than that of juvenile donors, who are better candidates for tissues damage repair.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.13205","type":"journal-article","created":{"date-parts":[[2019,8,16]],"date-time":"2019-08-16T18:39:10Z","timestamp":1565980750000},"page":"72-83","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Ageing affects the proliferation and mineralization of rat dental pulp stem cells under inflammatory conditions"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9986-8023","authenticated-orcid":false,"given":"T.","family":"Ning","sequence":"first","affiliation":[{"name":"Department of Stomatology Nanfang Hospital Southern Medical University Guangzhou China"},{"name":"College of Stomatology Southern Medical University Guangzhou China"},{"name":"Department of Endodontics Stomatological Hospital Southern Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Stomatology Guangzhou Hospital of Integrated Traditional and West Medicine Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Stomatology Nanfang Hospital Southern Medical University Guangzhou China"},{"name":"College of Stomatology Southern Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X.","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Stomatology Nanfang Hospital Southern Medical University Guangzhou China"},{"name":"College of Stomatology Southern Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X.","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Stomatology Nanfang Hospital Southern Medical University Guangzhou China"},{"name":"College of Stomatology Southern Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Stomatology Nanfang Hospital Southern Medical University Guangzhou China"},{"name":"College of Stomatology Southern Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Stomatology Southern Medical University Guangzhou China"},{"name":"Department of Endodontics Stomatological Hospital Southern Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9678-6962","authenticated-orcid":false,"given":"B.","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Stomatology Nanfang Hospital Southern Medical University Guangzhou China"},{"name":"College of Stomatology Southern Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4484-9455","authenticated-orcid":false,"given":"D.","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Stomatology Nanfang Hospital Southern Medical University Guangzhou China"},{"name":"College of Stomatology Southern Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,9,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2012.12.035"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/term.220"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scr.2011.11.002"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dental.2015.02.004"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1144090"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910508401105"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-015-2125-8"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2014.01.021"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2014.09.004"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.240309797"},{"key":"e_1_2_8_12_1","first-page":"1","article-title":"Dental pulp cells isolated from teeth with superficial caries retain an inflammatory phenotype and display an enhanced matrix mineralization potential","volume":"8","author":"Hanaa A","year":"2017","journal-title":"Frontiers in Physiology"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.24732"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2016.11.012"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-004-1035-y"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2014.3120"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.27472"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2014.01.020"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI68049"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbior.2018.06.003"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.113.20.3613"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.21061"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-016-0404-2"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10735-016-9672-5"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2014.03.015"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2009.07.016"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.035758"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/term.1686"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-017-0506-5"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034509334070"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2009.0315"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034515612086"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05862"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2013.08.012"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10522-008-9187-x"},{"key":"e_1_2_8_36_1","first-page":"1","article-title":"Interferon\u2010gamma improves impaired dentinogenic and immunosuppressive functions of irreversible pulpitis\u2010derived human dental pulp stem cells","volume":"6","author":"Sonoda S","year":"2017","journal-title":"Scientific Report"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2014.08.003"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-017-0633-z"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellbi.2009.03.006"},{"key":"e_1_2_8_40_1","first-page":"1","article-title":"Priming integrin alpha5 promotes human dental pulp stem cells odontogenic differentiation due to extracellular matrix deposition and amplified extracellular matrix\u2010receptor activity","volume":"234","author":"Wang H","year":"2018","journal-title":"Journal of Cell Physiology"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/iub.1159"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2121-11-32"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/sctm.17-0033"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2009.080705"}],"container-title":["International Endodontic Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fiej.13205","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iej.13205","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/iej.13205","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iej.13205","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T14:12:27Z","timestamp":1694182347000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/iej.13205"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,18]]},"references-count":43,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1]]}},"alternative-id":["10.1111\/iej.13205"],"URL":"https:\/\/doi.org\/10.1111\/iej.13205","archive":["Portico"],"relation":{},"ISSN":["0143-2885","1365-2591"],"issn-type":[{"value":"0143-2885","type":"print"},{"value":"1365-2591","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9,18]]},"assertion":[{"value":"2019-04-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-09-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}