{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T12:50:03Z","timestamp":1791377403217,"version":"4.3.2"},"reference-count":25,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,7,21]],"date-time":"2020-07-21T00:00:00Z","timestamp":1595289600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To explore a set of inflammatory biomarkers obtained from dentinal fluid (DF) from patients with symptomatic irreversible pulpitis (IP), reversible pulpitis (RP) and normal pulp (NP).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methodology<\/jats:title>\n                    <jats:p>\n                      A cross\u2010sectional exploratory study was performed, recruiting 64 patients on the basis of their respective pulp condition. DF samples were obtained from all patients (23, from IP patients; 20, from RP patients; and 21, from NP patients). Quantification of biomarkers was performed using a Luminex\u00ae MAGPIX platform system and multiplex assay kits. The Kruskal\u2013Wallis test was used for comparisons with regard to pulp state. A simple logistic regression model and the odds ratio (OR) with a 95% level of confidence (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.05) were used to evaluate associations between biomarker levels and pulpal diagnosis. The performance discrimination of the biomarkers was evaluated through the construction of a receiver operating characteristic (ROC) curve by calculating the area under the curve (AUC) for IP versus RP after logistic regression modelling. Youden criteria were used to establish cut\u2010off points for biomarkers alone with AUC\u00a0&gt;\u00a070 and\n                      <jats:italic>P<\/jats:italic>\n                      \u2010value\u00a0&lt;\u00a00.05, or estimated probabilities from the multivariable logistic model.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The biomarkers that had significantly higher values in participants with IP versus RP were IL\u20101\u03b1, VEGF\u2010\u03b1 and FGF acid (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05). FGF acid (OR: 12.62;\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.0085; CI 95% 1.91\u201383.29) and VEGF\u2010\u03b1 (OR: 2.61;\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.0252; CI 95% 1.13\u20136.03) were associated with pulp diagnoses of IP versus RP. The AUC\u2010ROC curve for FGF acid was 0.79. The model containing FGF acid, IL\u20101\u03b1, IL\u20106 and TIMP\u20101 had an AUC\u2010ROC of 0.92 for IP versus RP with a significant difference from the FGF acid ROC curve (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.0231).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Dentinal fluid could be used to assay pulpal mediators in the molecular diagnosis of pulpitis. Despite the limitation of the clinical diagnostics used in the present study, it was possible to detect a difference between irreversible symptomatic pulpitis and reversible pulpitis associated with the following combined biomarkers: FGF acid\u00a0+\u00a0IL\u20106\u00a0+\u00a0IL\u20101\u03b1, +TIMP\u20101.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/iej.13343","type":"journal-article","created":{"date-parts":[[2020,6,4]],"date-time":"2020-06-04T11:44:58Z","timestamp":1591271098000},"page":"1181-1191","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["Inflammatory biomarkers in dentinal fluid as an approach to molecular diagnostics in pulpitis"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6610-8183","authenticated-orcid":false,"given":"C.","family":"Brizuela","sequence":"first","affiliation":[{"name":"Dental School Universidad de Los Andes Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2119-1363","authenticated-orcid":false,"given":"G.","family":"Meza","sequence":"additional","affiliation":[{"name":"Dental School Universidad de Los Andes Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1303-3787","authenticated-orcid":false,"given":"M.","family":"Mercad\u00e9","sequence":"additional","affiliation":[{"name":"Dental School Universitat de Barcelona Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Inostroza","sequence":"additional","affiliation":[{"name":"Dental School Universidad de Los Andes Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Chaparro","sequence":"additional","affiliation":[{"name":"Dental School Universidad de Los Andes Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Bravo","sequence":"additional","affiliation":[{"name":"Dental School Universidad de Los Andes Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Brice\u00f1o","sequence":"additional","affiliation":[{"name":"Dental School Universidad de Los Andes Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8289-7314","authenticated-orcid":false,"given":"M.","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology and Medicine &amp; Laboratory of Periodontal Biology Faculty of Dentistry Universidad de Chile Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Giner","sequence":"additional","affiliation":[{"name":"Facultad de Odontolog\u00eda Universidad Internacional de Catalu\u00f1a (UIC) UIC\u2010Barcelona Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Dental School Universidad de Los Andes Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,7,21]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"55","article-title":"Dental pulp neurophysiology: part 1. 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