{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T06:02:29Z","timestamp":1791525749392,"version":"4.3.4"},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,10,7]],"date-time":"2020-10-07T00:00:00Z","timestamp":1602028800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To validate a new method for the evaluation of pulp tissue debridement in the root canals of extracted teeth using an impregnation protocol involving potassium triiodide, a radiocontrast solution known as Lugol\u2019s, combined with micro\u2010computed tomographic (micro\u2010CT) imaging.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>The impact of NaOCl on the radiopacity of Lugol\u2019s solution was assessed using a two\u2010fold dilution series of Lugol in distilled water and 5.25% NaOCl, which were then pipetted into transparent dishes and radiographed. To verify the influence of Lugol on the proteolytic effect of NaOCl, a dissolution test was performed using fresh bovine meat. Ten slices did not undergo any tissue processing, whilst twenty slices were fixed in formaldehyde for 24\u00a0h. After that, 10 of them were immersed in Lugol for another 24\u00a0h. Then, all specimens were placed in NaOCl and the time required for a complete tissue dissolution was recorded. For the last experiments (histological validation and micro\u2010CT assessment), 8 extracted mandibular premolars with formerly vital pulps were immersed in buffered formalin, scanned in a micro\u2010CT device, accessed, immersed in Lugol for 7\u00a0days and scanned again. Then, the root canals of 5 teeth were prepared and scanned, and the volume of remaining pulp tissue identified and quantified, whilst 3 teeth were histologically processed. The same specimens were subjected to histological assessment, and the images of the histologic sections were registered with the corresponding micro\u2010CT images to verify whether the pulp tissue in the histological sections matched its counterpart in the Lugol\u2010impregnated tissues identified in the micro\u2010CT slices.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>There was no discernible effect on radiopacity when NaOCl was mixed with Lugol\u2019s solution. Tissue processing did not affect the time required for the complete dissolution of fresh bovine meat. Histological evaluation revealed a correlation between micro\u2010CT and histological images confirming the identification of Lugol\u2010impregnated pulp tissue in micro\u2010CT images.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The radiocontrast Lugol\u2019s solution was unaffected by NaOCl and did not interfere with its soft tissue dissolution capability. The impregnation protocol using Lugol\u2019s solution allowed the visualization of pulp tissue on the micro\u2010CT images and the identification of pulp remnants after chemical\u2013mechanical canal procedures.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.13408","type":"journal-article","created":{"date-parts":[[2020,10,8]],"date-time":"2020-10-08T07:11:39Z","timestamp":1602141099000},"page":"279-293","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Contrast\u2010enhanced micro\u2010CT to assess dental pulp tissue debridement in root canals of extracted teeth: a series of cascading experiments towards method validation"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7778-047X","authenticated-orcid":false,"given":"G.","family":"De\u2010Deus","sequence":"first","affiliation":[{"name":"Department of Endodontics Fluminense Federal University Niter\u00f3i Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9972-6861","authenticated-orcid":false,"given":"F. 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L.","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Endodontics Fluminense Federal University Niter\u00f3i Rio de Janeiro Brazil"},{"name":"Department of Endodontics Grande Rio University Duque de Caxias Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7015-9899","authenticated-orcid":false,"given":"J. C. A.","family":"Carvalhal","sequence":"additional","affiliation":[{"name":"Department of Endodontics Fluminense Federal University Niter\u00f3i Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. Q.","family":"Zamolyi","sequence":"additional","affiliation":[{"name":"Bonsucesso Federal Hospital Rio de Janeiro Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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