{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T21:11:12Z","timestamp":1788297072324,"version":"build-2803163510"},"reference-count":41,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,10,17]],"date-time":"2020-10-17T00:00:00Z","timestamp":1602892800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81700951"],"award-info":[{"award-number":["81700951"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81771095"],"award-info":[{"award-number":["81771095"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81803186"],"award-info":[{"award-number":["81803186"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To investigate the effect of miR\u2010223 on NLRP3, subsequently regulating the production of the NLRP3\/CASP1 inflammasome pathway\u2010mediated proinflammatory cytokines IL\u20101\u03b2 and IL\u201018 in human dental pulp fibroblasts (HDPFs).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methodology<\/jats:title>\n                    <jats:p>\n                      Human dental pulp tissue (HDPT) and HDPFs were obtained from impacted third molars. The miR\u2010223 mimics and inhibitor or NLRP3 plasmid were used to upregulate or downregulate miR\u2010223 or NLRP3 in HDPFs, respectively. Computational prediction via TargetScan 5.1 and a luciferase reporter assay was conducted to confirm target association. The mRNA and protein expression of NLRP3, caspase\u20101, IL\u20101\u03b2 and IL\u201018 was determined by qRT\u2010PCR and Western blotting, respectively. The release of IL\u20101\u03b2 and IL\u201018 was analysed by ELISA. The significance of the differences between the experimental and the control groups was determined using one\u2010way analysis of variance;\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05 indicated statistical significance.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A decrease in miR\u2010223 and an increase in NLRP3 in HDPT occurred during the transformation of reversible pulpitis into irreversible pulpitis compared to that in healthy pulp tissue (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05). The computational prediction and luciferase reporter assay confirmed that NLRP3 was a direct target of miR\u2010223 in HDPFs. The miR\u2010223 inhibitor further promoted ATP plus LPS\u2010induced NLRP3\/CASP1 inflammasome pathway activation compared to the ATP plus LPS\u2010induced group (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05). In contrast, the miR\u2010223 mimic significantly inhibited the NLRP3\/CASP1 inflammasome pathway activation induced by ATP plus LPS compared to the ATP plus LPS\u2010induced group (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>MiR\u2010223 served as a negative regulator involved in the control of the production and secretion of proinflammatory cytokines mediated by the NLRP3\/CASP1 inflammasome pathway by targeting NLRP3. These data provide insight into the potential regulatory effects of miRNAs on the NLRP3 inflammasome, thus opening up novel potential therapeutic avenues for future endodontic treatment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/iej.13413","type":"journal-article","created":{"date-parts":[[2020,9,23]],"date-time":"2020-09-23T15:21:25Z","timestamp":1600874485000},"page":"241-254","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["MicroRNA\u2010223 negatively regulates LPS\u2010induced inflammatory responses by targeting NLRP3 in human dental pulp fibroblasts"],"prefix":"10.1111","volume":"54","author":[{"given":"D.","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Military Stomatology &amp; National Clinical Research Center for Oral Diseases &amp; Shaanxi Key Laboratory of Stomatology Department of Operative Dentistry &amp; Endodontics School of Stomatology Fourth Military Medical University Xi\u2019an China"},{"name":"Department of Occupational and Environmental Health and the Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment School of Public Health Fourth Military Medical University Xi\u2019an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Military Stomatology &amp; National Clinical Research Center for Oral Diseases &amp; Shaanxi Clinical Research Center for Oral Diseases Department of Pediatric Dentistry School of Stomatology Fourth Military Medical University Xi\u2019an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Xue","sequence":"additional","affiliation":[{"name":"Department of Occupational and Environmental Health and the Ministry of Education Key Lab of Hazard Assessment and Control in Special Operational Environment School of Public 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