{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T12:50:03Z","timestamp":1791377403358,"version":"4.3.2"},"reference-count":46,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,12,13]],"date-time":"2020-12-13T00:00:00Z","timestamp":1607817600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["3\/2\/jan\u20102018\/PG \u2013 Thesis\u2010HDR (48)"],"award-info":[{"award-number":["3\/2\/jan\u20102018\/PG \u2013 Thesis\u2010HDR (48)"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To investigate the correlation between the concentration of active\u2010matrix metalloproteinases\u20109 (aMMP\u20109) in pulpal blood and the outcome of pulpotomy in mature permanent teeth with symptomatic irreversible pulpitis (SIP).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>Forty permanent molar teeth with a clinical diagnosis of SIP and normal apical tissues with periapical index (PAI) score\u00a0\u2264\u00a02 and ten permanent teeth (8 molars and two premolars) with a diagnosis of normal pulp that required root canal treatment for prosthetic reasons from patients between the ages of 15\u201335\u00a0years were recruited. All clinical procedures were performed under local anaesthesia and rubber dam isolation. After access opening, the coronal pulp tissue was amputated up to the canal orifice. A 100\u00a0\u03bcL volume of the pulpal blood was collected using a micropipette and transported to the laboratory. Sodium hypochlorite (2.5 %) was used as a haemostatic agent, and mineral trioxide aggregate (MTA) was used as the pulp capping material. The tooth was restored with composite at the same visit. Teeth with normal pulps were treated with single\u2010visit root canal treatment. Patients with pulpotomy were recalled at 6 and 12\u00a0months. Outcome assessment of teeth with pulpotomy was carried out at 12\u00a0months and was categorized as success (asymptomatic patients with PAI score\u00a0\u2264\u00a02) or failure (symptomatic patients or PAI score\u00a0\u2265\u00a03). Quantification of aMMP\u20109 in pulpal blood was achieved using a fluorometric assay. The following statistical analyses were performed to assess the data: t\u2010test, Fisher's exact test, kappa coefficient, non\u2010parametric test, Wilcoxon rank\u2010sum test, Spearman rank correlation test and receiver operating characteristic curve (ROC).<\/jats:p><\/jats:sec><jats:sec><jats:title>Result<\/jats:title><jats:p>The success rate of pulpotomy was 88 % at 12\u2010months. There was a significant difference between the median concentrations of aMMP\u20109 in pulpal blood of teeth with normal pulps (52 (12\u201396)\u00a0ng\u00a0mL<jats:sup>\u22121<\/jats:sup>:) and SIP (193.3 (25.8\u2013607.7)\u00a0ng\u00a0mL<jats:sup>\u22121<\/jats:sup>:) (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.0003) and successful (132.3 (25.8\u2013548.3)\u00a0ng\u00a0mL<jats:sup>\u22121<\/jats:sup>:) and failed cases (512.4 (334.8\u2013607.7\u00a0ng\u00a0mL<jats:sup>\u22121<\/jats:sup>:) (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.0015) of MTA pulpotomy. A significant association was established between aMMP\u20109 concentration and outcome of pulpotomy. The area under the receiver operating characteristics curve (0.9484, 95%CI) suggested excellent discriminatory power of aMMP\u20109 concentration in pulpal blood to predict the pulpotomy outcome.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The pulpal blood concentration of aMMP\u20109 was significantly associated with the outcome of pulpotomy in teeth with symptomatic irreversible pulpitis, where it may be used as a potential prognostic biomarker.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.13437","type":"journal-article","created":{"date-parts":[[2020,10,31]],"date-time":"2020-10-31T02:33:22Z","timestamp":1604111602000},"page":"479-489","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":54,"title":["Association between concentration of active MMP\u20109 in pulpal blood and pulpotomy outcome in permanent mature teeth with irreversible pulpitis \u2013 a preliminary study"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9192-8781","authenticated-orcid":false,"given":"R.","family":"Sharma","sequence":"first","affiliation":[{"name":"Division of Conservative Dentistry and Endodontics Centre for Dental Education and Research All India Institute of Medical Sciences New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4941-7053","authenticated-orcid":false,"given":"V.","family":"Kumar","sequence":"additional","affiliation":[{"name":"Division of Conservative Dentistry and Endodontics Centre for Dental Education and Research All India Institute of Medical Sciences New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Logani","sequence":"additional","affiliation":[{"name":"Division of Conservative Dentistry and Endodontics Centre for Dental Education and Research All India Institute of Medical Sciences New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Chawla","sequence":"additional","affiliation":[{"name":"Division of Conservative Dentistry and Endodontics Centre for Dental Education and Research All India Institute of Medical Sciences New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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