{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T10:51:39Z","timestamp":1790506299424,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,3,31]],"date-time":"2021-03-31T00:00:00Z","timestamp":1617148800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81970925"],"award-info":[{"award-number":["81970925"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2017A030308011"],"award-info":[{"award-number":["2017A030308011"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To profile molecular changes in lipopolysaccharide (LPS)\u2010induced experimental pulpitis in a rat model and explore the feasibility of a molecular\u2010based diagnostic strategy for pulpitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>Seventy\u2010three maxillary incisors of Sprague\u2010Dawley rats were used to establish pulpitis models with LPS. Inflammatory grading was performed in four equal sections of the pulp divided from the injured site to the root apex. An antibody array was used to compare the expression of 67 molecules between control pulp and inflamed pulp 12 and 72\u00a0h after LPS application. The levels of differentially expressed molecules in the control and inflamed pulp (collected at 3, 6, 9, 12, 24 and 72\u00a0h after LPS treatment) were examined via ELISA, and correlations between inflammatory scores and molecule expression were assessed. The molecule distributions in the pulp were investigated by immunofluorescence staining. Data were analysed with paired <jats:italic>t<\/jats:italic>\u2010test, one\u2010way <jats:sc>anova<\/jats:sc>, Kruskal\u2013Wallis tests, and Spearman\u2019s and Pearson\u2019s correlations with significance set at <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Polymorphonuclear neutrophils were observed in the injured site 3\u00a0h after LPS stimulation. Inflammatory infiltration peaked at 12\u00a0h and was limited to the injured site with osteodentine deposition at 72\u00a0h. Thirteen molecules were significantly differentially expressed between the control and LPS\u2010injured pulp. ELISA validated that tissue inhibitor of metalloproteinase\u20101 (TIMP\u20101) expression dramatically peaked at 12\u00a0h (compared with other time points, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) and returned to baseline at 72\u00a0h. The TIMP\u20101 concentration was strongly correlated with inflammation severity in the apical three\u2010quarters of the pulp, and the strongest correlation was found in the lower\u2010middle quarter (<jats:italic>r<\/jats:italic>\u00a0=\u00a00.786, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001). Immunofluorescence staining revealed that in the apical three\u2010quarters of the pulp, TIMP\u20101 expression was significantly higher in the 12\u00a0h group than in the control and 3, 6, 24 and 72\u00a0h groups (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study provides a molecular profile of LPS\u2010induced pulpitis in a rat model. TIMP\u20101 had a strong positive correlation with the severity of dental pulp inflammation, verifying the feasibility of applying biomarkers to identify specific pathological conditions in pulpitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.13514","type":"journal-article","created":{"date-parts":[[2021,3,14]],"date-time":"2021-03-14T12:20:50Z","timestamp":1615724450000},"page":"1328-1341","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Histology\u2010based profile of inflammatory mediators in experimentally induced pulpitis in a rat model: screening for possible biomarkers"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5065-5847","authenticated-orcid":false,"given":"M.","family":"Li","sequence":"first","affiliation":[{"name":"Department of Operative Dentistry and Endodontics Guanghua School of Stomatology Hospital of Stomatology Sun Yat\u2010sen University Guangzhou 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