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In the CAP group, sterile cotton wool containing 10<jats:sup>8<\/jats:sup> colony\u2010forming units of <jats:italic>P<\/jats:italic>.\u00a0<jats:italic>gingivalis<\/jats:italic> was placed into the pulp chamber after pulp exposure followed by coronal resin filling in bilateral maxillary first and second molars. The mice were fed with a chow diet to induce atherosclerosis. Animals were euthanized 16\u00a0weeks after the operation, and the periapical lesions of bilateral maxillary first and second molars were assessed by micro\u2010CT. After collection of aortic arches, atherosclerotic lesions were measured by Oil Red O staining. Serum levels of high\u2010density lipoprotein cholesterol (HDL\u2010C), low\u2010density lipoprotein cholesterol (LDL\u2010C), total cholesterol (TC), and triglycerides (TG) were measured. Stools were collected to detect alterations in gut microbiota by 16S rRNA gene sequencing. Independent samples <jats:italic>t<\/jats:italic>\u2010test was used to calculate the difference between the two groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CAP was observed in 98.2% of molars. A significant increase in atherosclerotic plaque formation in the aortic arches was found in the CAP groups (CAP: 2.001%\u00a0\u00b1\u00a00.27%, control: 0.927%\u00a0\u00b1\u00a00.22%, <jats:italic>p<\/jats:italic>\u00a0=\u00a0.005). No significant difference was observed between sevum level of HDL\u2010C (CAP: 2.295 \u00b1 0.31 mmol\/L, Control: 3.037 \u00b1 0.55 mmol\/L, <jats:italic>p<\/jats:italic> = .264) or LDL\u2010C (CAP: 17.066 \u00b1 3.95 mmol\/L, Control: 10.948 \u00b1 1.69 mmol\/L, <jats:italic>p<\/jats:italic> = .177) in CAP group and Control group. There were no significant differences in TG (CAP: 1.076\u00a0\u00b1\u00a00.08\u00a0mmol\/L, control: 1.034\u00a0\u00b1\u00a00.13\u00a0mmol\/L, <jats:italic>p<\/jats:italic>\u00a0=\u00a0.794) or TC (CAP: 6.372\u00a0\u00b1\u00a00.98\u00a0mmol\/L, control: 6.679\u00a0\u00b1\u00a00.75\u00a0mmol\/L, <jats:italic>p<\/jats:italic>\u00a0=\u00a0.72) levels between the two groups (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a0.05). The alpha diversity was elevated in the CAP group. In terms of beta diversity, the CAP and control groups were clearly distinguished by the microbial community.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In a mouse experimental model, pulp infection with <jats:italic>P<\/jats:italic>.\u00a0<jats:italic>gingivalis<\/jats:italic> \u2010induced CAP, thus aggravating the development of atherosclerosis. Meanwhile, CAP increased alpha diversity and altered the beta diversity of the gut microbiota.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.13655","type":"journal-article","created":{"date-parts":[[2021,10,29]],"date-time":"2021-10-29T13:12:01Z","timestamp":1635513121000},"page":"152-163","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Chronic apical periodontitis exacerbates atherosclerosis in apolipoprotein E\u2010deficient mice and leads to changes in the diversity of gut microbiota"],"prefix":"10.1111","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3189-9915","authenticated-orcid":false,"given":"Guowu","family":"Gan","sequence":"first","affiliation":[{"name":"Fujian Key Laboratory of Oral Diseases &amp; Fujian Provincial Engineering Research Center of Oral Biomaterial &amp; Stomatological Key lab of Fujian College and University School and Hospital of Stomatology Fujian Medical University  Fuzhou China"},{"name":"Institute of Stomatology, Research Center of Dental and Craniofacial Implants School and Hospital of Stomatology Fujian Medical University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7588-1979","authenticated-orcid":false,"given":"Beibei","family":"Lu","sequence":"additional","affiliation":[{"name":"Fujian Key Laboratory of Oral Diseases &amp; Fujian Provincial Engineering Research Center of Oral Biomaterial &amp; Stomatological Key lab of Fujian College and University School and Hospital of Stomatology Fujian Medical University  Fuzhou China"},{"name":"Institute of Stomatology, Research Center of Dental and Craniofacial Implants School and Hospital of Stomatology Fujian Medical University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren","family":"Zhang","sequence":"additional","affiliation":[{"name":"Fujian Key Laboratory of Oral Diseases &amp; Fujian Provincial Engineering Research Center of Oral Biomaterial &amp; Stomatological Key lab of Fujian College and University School and Hospital of Stomatology Fujian Medical University  Fuzhou China"},{"name":"Institute of Stomatology, Research Center of Dental and Craniofacial Implants School and Hospital of Stomatology Fujian Medical University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufang","family":"Luo","sequence":"additional","affiliation":[{"name":"Fujian Key Laboratory of Oral Diseases &amp; Fujian Provincial Engineering Research Center of Oral Biomaterial &amp; Stomatological Key lab of Fujian College and University School and Hospital of Stomatology Fujian Medical University  Fuzhou China"},{"name":"Institute of Stomatology, Research Center of Dental and Craniofacial Implants School and Hospital of Stomatology Fujian Medical University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6619-2485","authenticated-orcid":false,"given":"Shuai","family":"Chen","sequence":"additional","affiliation":[{"name":"Fujian Key Laboratory of Oral Diseases &amp; Fujian Provincial Engineering Research Center of Oral Biomaterial &amp; Stomatological Key lab of Fujian College and University School and Hospital of Stomatology Fujian Medical University  Fuzhou China"},{"name":"Institute of Stomatology, Research Center of Dental and Craniofacial Implants School and Hospital of Stomatology Fujian Medical University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7261-7128","authenticated-orcid":false,"given":"Huaxiang","family":"Lei","sequence":"additional","affiliation":[{"name":"Fujian Key Laboratory of Oral Diseases &amp; 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