{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T18:27:59Z","timestamp":1783708079313,"version":"3.55.0"},"reference-count":29,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,3,28]],"date-time":"2022-03-28T00:00:00Z","timestamp":1648425600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81700955"],"award-info":[{"award-number":["81700955"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>This study aimed to investigate the effect of the circadian clock gene <jats:italic>Bmal1<\/jats:italic> on dentinogenic differentiation of dental pulp stem cells (DPSCs) under inflammatory conditions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>Dental pulp stem cells were isolated from the pulp tissue of the healthy donor and were then stimulated with different concentrations of lipopolysaccharide (LPS) to mimic inflammatory conditions. Real\u2010time polymerase chain reaction was used to detect the gene expression of circadian clock genes <jats:italic>Bmal1<\/jats:italic>, <jats:italic>Clock<\/jats:italic>, <jats:italic>Per1<\/jats:italic>, <jats:italic>Per2<\/jats:italic>, <jats:italic>Cry1<\/jats:italic>, and <jats:italic>Cry2<\/jats:italic>. Western blot (WB) was applied to analyse the protein expression of circadian clock proteins (BMAL1, CLOCK) and dentinogenic differentiation\u2010related proteins (DSPP, DMP1). In addition, the apoptosis and osteogenic differentiation of DPSCs were also analysed in the presence of different concentrations of LPS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The expression of circadian clock genes of DPSCs significantly changed in an inflammatory environment. WB analysis shows that BMAL1 is relevant to the dentinogenic differentiation of DPSCs. In low concentrations of LPS\u2010mimicked inflammatory conditions, the expression of BMAL1 increased and promoted the dentinogenic differentiation of DPSCs. However, under high concentrations of LPS\u2010mimicked inflammatory conditions, the expression of BMAL1 decreased and inhibited the dentinogenic differentiation of DPSCs. Moreover, the effects of BMAL1 on dentinogenic differentiation of DPSCs may be through PI3K\/Akt\/mTOR pathway.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study showed that the circadian clock gene <jats:italic>Bmal1<\/jats:italic> affected dentinogenic differentiation of DPSCs, providing a new insight for clinical stem cell\u2010based restorative dentinogenesis therapies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.13720","type":"journal-article","created":{"date-parts":[[2022,3,9]],"date-time":"2022-03-09T22:08:49Z","timestamp":1646863729000},"page":"505-516","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Effects of BMAL1 on dentinogenic differentiation of dental pulp stem cells via PI3K\/Akt\/mTOR pathway"],"prefix":"10.1111","volume":"55","author":[{"given":"Hui","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Stomatology Union Hospital Tongji 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