{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T17:05:26Z","timestamp":1791824726828,"version":"4.3.4"},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,11,12]],"date-time":"2022-11-12T00:00:00Z","timestamp":1668211200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004035","name":"Jordan University of Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004035","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To evaluate the expression and function of the nod\u2010like receptor pyrin domain containing 3 (NLRP3) inflammasome in caries induced pulpitis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methodology<\/jats:title>\n                    <jats:p>\n                      NLRP3 expression was determined with immunohistochemistry in the dental pulp and qPCR in dental pulp cells (DPCs). THP\u20101 macrophages expressing the apoptosis\u2010related speck\u2010like protein (ASC) and green fluorescent protein (GFP) fusion protein were used to assess NLRP3 inflammasome activation by live cell imaging, following treatment with lipopolysaccharide (LPS) and lipoteichoic acid (LTA). Caspase I inhibitor was used to confirm inflammasome activation. An\n                      <jats:italic>ex\u2010vivo<\/jats:italic>\n                      pulpitis model in which the DPCs were co\u2010cultured with THP\u20101 macrophages was used to study the effect of the NLRP3 inflammasome inhibitor (MCC950), and cytokines were measured using ELISA and multiplex array. Data were analysed using the\n                      <jats:italic>t\u2010test<\/jats:italic>\n                      or\n                      <jats:sc>anova<\/jats:sc>\n                      followed by a Bonferroni\n                      <jats:italic>post hoc<\/jats:italic>\n                      test with the level of significance set at\n                      <jats:italic>p<\/jats:italic>\n                      \u2009\u2264\u2009.05.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      NLRP3 inflammasome was differentially expressed in dental pulp of sound and carious teeth. Treatment of DPCs with LTA significantly upregulates NLRP3 and IL\u20101 \u03b2\u2010expression (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.05) and in induces more ASC specks formation compared to LPS. IL\u2010\u03b2 release in response to LTA treatment is significantly reduced with Caspase I inhibitor suggesting inflammasome dependent mechanism (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.01). NLRP3\u2010specific inhibitor, MCC950, significantly reduced IL\u20101\u03b2 and IL\u20106 in an\n                      <jats:italic>ex\u2010vivo<\/jats:italic>\n                      pulpitis model (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.01) but had no effect on IL\u20108 or matrix metalloproteinase\u20109 (MMP\u20109).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Expression and upregulation of NLRP3 inflammasome with caries and LTA treatment suggest a role in caries\u2010induced pulpitis. NLRP3 inhibitor attenuated the release of selective inflammatory cytokines and could be a potential treatment target that merit further investigation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/iej.13855","type":"journal-article","created":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T10:05:08Z","timestamp":1666778708000},"page":"193-202","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Regulation of caries\u2010induced pulp inflammation by NLRP3 inflammasome: A laboratory\u2010based investigation"],"prefix":"10.1111","volume":"56","author":[{"given":"Banan","family":"Al Natour","sequence":"first","affiliation":[{"name":"Wellcome\u2010Wolfson Institute for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences Queen's University Belfast  Belfast UK"},{"name":"Department of Oral Medicine and Surgery Jordan University of Science and Technology  Irbid 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