{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T11:19:14Z","timestamp":1787224754700,"version":"build-2736575974"},"reference-count":56,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,1,19]],"date-time":"2023-01-19T00:00:00Z","timestamp":1674086400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004704","name":"National Research Council of Thailand","doi-asserted-by":"publisher","award":["N41A640135"],"award-info":[{"award-number":["N41A640135"]}],"id":[{"id":"10.13039\/501100004704","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To investigate the effect of Wnt3a on odonto\/osteogenic differentiation of stem cells isolated from human exfoliated deciduous teeth (SHEDs) and reparative dentine formation in a rat model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methodology<\/jats:title>\n                    <jats:p>\n                      Stem cells isolated from human exfoliated deciduous teeth were cultured in media with Wnt3a (50\u2013200\u2009ng\/ml). Wnt activation was confirmed by \u03b2\u2010catenin immunocytochemistry. Colony\u2010forming unit assay (normalized percentage area), osteogenic gene expression analysis by real\u2010time polymerase chain reaction and mineralization assays measured by the absorption at 540\u2009nm were performed. Tertiary dentine formation\n                      <jats:italic>in vivo<\/jats:italic>\n                      was evaluated using 8\u2010week\u2010old, male Wistar rats. Cavities with pinpoint pulp exposure by a sharp instrument were prepared at the mesial surface of the first molars. Teeth were divided into (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a06): (1) distilled water (negative control), (2) phosphate\u2010buffered saline (PBS), (3) lithium chloride in DI (20\u2009\u03bcM), and (4) Wnt3a in PBS (200\u2009ng\/ml). Collagen sponge was used as a scaffold. The cavity was sealed with glass ionomer restoration. Four weeks later, animals were euthanized by sodium pentobarbital (120\u2009mg\/kg body weight). Hard tissue formation was evaluated using micro\u2010computerized tomography. Sixty consecutive slides from the initial plane were analysed and calculated as bone\/dentine volume per total tissue volume. Paraffin sections (2\u00a0\u03bcm) were stained with haematoxylin and eosin and Masson's trichrome for morphological evaluation. Data are presented as the mean\u2009\u00b1\u2009standard error. Mann\u2013Whitney\n                      <jats:italic>U<\/jats:italic>\n                      test was used for two\u2010group comparison. Kruskal Wallis followed by pairwise comparison was employed for three or more group comparisons. Statistical analysis was performed using GraphPad Prism 7. Differences were considered significant at\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.05.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Wnt3a decreased SHEDs colony formation and increased\n                      <jats:italic>OSX<\/jats:italic>\n                      ,\n                      <jats:italic>BMP2<\/jats:italic>\n                      , and\n                      <jats:italic>DMP1<\/jats:italic>\n                      expression, corresponding to an increase in mineralization. Additionally, a significant increase in dentine\/bone volume per total tissue volume was observed in Wnt3a treated defects. Dentine bridge formation at the exposure sites treated with Wnt3a demonstrated, while fibrous tissues were observed in the control.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Wnt3a suppressed proliferation, increased osteogenic differentiation of SHEDs and promotes tertiary dentine formation. Wnt3a could be utilized as biological molecule for vital pulp therapy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/iej.13888","type":"journal-article","created":{"date-parts":[[2023,1,12]],"date-time":"2023-01-12T09:51:02Z","timestamp":1673517062000},"page":"514-529","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Wnt3a promotes odonto\/osteogenic differentiation\n                    <i>in vitro<\/i>\n                    and tertiary dentin formation in a rat model"],"prefix":"10.1111","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0243-0712","authenticated-orcid":false,"given":"Waleerat","family":"Sukarawan","sequence":"first","affiliation":[{"name":"Dental Stem Cell Biology Research Unit, Faculty of Dentistry Chulalongkorn University  Bangkok Thailand"},{"name":"Department of Pediatric Dentistry, Faculty of Dentistry Chulalongkorn University  Bangkok 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