{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T11:14:10Z","timestamp":1784891650961,"version":"3.55.0"},"reference-count":54,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2023,5,2]],"date-time":"2023-05-02T00:00:00Z","timestamp":1682985600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To investigate the regulatory role of miR\u2010155 and Kinesin Superfamily Proteins\u20105C (KIF\u20105C) in the progression of pulpitis based on bioinformatic analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>Normal pulp tissues and pulpitis pulp tissues were collected and subjected to high\u2010throughput sequencing and the differentially expressed miRNAs were determined. An <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> pulpitis model was established. HE, IHC staining and histological evaluation were used to verify the inflammatory state of human and mouse pulp tissues. The mRNA expression of IL\u20101\u03b2 and TGF\u2010\u03b21 were determined by RT\u2010qPCR and protein expression of IL\u20101\u03b1, IL\u20104, IL\u20108, IL\u201013, IFN\u2010\u03b3, IL\u20106, IL\u201010 and MCP\u20101 were determined by protein chip. The target genes of miR\u2010155 were predicted by miRanda database and verified by Dual\u2010luciferase reporter assay, RT\u2010qPCR and western blotting. MiR\u2010155 lentivirus were used to upregulate or downregulate miR\u2010155 and the siRNA of KIF\u20105C was used to downregulate KIF\u20105C. The expression of miR\u2010155 or KIF\u20105C was determined by RT\u2010qPCR. All statistics were analysed using GraphPad prism 8.2.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The high\u2010throughput sequencing results showed that 6 miRNAs (miR\u2010155, miR\u201021, miR\u2010142, miR\u2010223, miR\u2010486, miR\u2010675) were significantly upregulated in diseased human pulp tissues, and miR\u2010155 was significantly elevated among the six miRNAs. RT\u2010qPCR results demonstrated that miR\u2010155 expression was upregulated in human pulpitic tissue, mice pulpitic tissue and LPS\u2010HDPCs. IL\u20101\u03b2 was increased while TGF\u2010\u03b21 was decreased in lenti\u2010miR\u2010155 transfected LPS\u2010HDPCs. Analysis of protein chip results indicated that lenti\u2010miR\u2010155 transfected LPS\u2010HDPCs produced higher levels of IL\u20108, IL\u20106, MCP\u20101. The opposite results were obtained when miR\u2010155 was inhibited. Through miRanda database screen and Dual\u2010luciferase reporter assay, the target gene (KIF\u20105C) of miR\u2010155 was identified. In lenti\u2010miR\u2010155 transfected LPS\u2010HDPCs, the expression of KIF\u20105C was downregulated. However, when shRNA\u2010miR\u2010155 was transfected to LPS\u2010HDPCs, the opposite result was obtained. Silent RNA was used to knock down KIF\u20105C, the results showed that when both KIF\u20105C and miR\u2010155 were knocked down simultaneously, the downregulated expression of inflammatory factors observed in LPS\u2010HDPCs following miR\u2010155 knockdown was rescued.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>MiR\u2010155 plays an important role in promoting pulpitis through targeting KIF\u20105C and may serve as a potential therapeutic target.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.13923","type":"journal-article","created":{"date-parts":[[2023,4,18]],"date-time":"2023-04-18T10:50:53Z","timestamp":1681815053000},"page":"837-853","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Overexpression of <scp>MicroRNA<\/scp>\u2010155 aggravates pulpitis by targeting kinesin superfamily <scp>Proteins\u20105C<\/scp> based on illumina <scp>high\u2010throughput<\/scp> sequencing"],"prefix":"10.1111","volume":"56","author":[{"given":"Ye","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Endodontics Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"He","sequence":"additional","affiliation":[{"name":"Department of Endodontics Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Endodontics Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Endodontics Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenmian","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Endodontics Affiliated Stomatology Hospital of Guangzhou Medical University, Guangdong Engineering Research Center of Oral Restoration and Reconstruction, Guangzhou Key Laboratory of Basic and Applied Research of Oral Regenerative 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