{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T19:38:50Z","timestamp":1784230730976,"version":"3.55.0"},"reference-count":59,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2023,5,6]],"date-time":"2023-05-06T00:00:00Z","timestamp":1683331200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004829","name":"Department of Science and Technology of Sichuan Province","doi-asserted-by":"publisher","award":["2020YFQ0008"],"award-info":[{"award-number":["2020YFQ0008"]}],"id":[{"id":"10.13039\/501100004829","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004829","name":"Department of Science and Technology of Sichuan Province","doi-asserted-by":"publisher","award":["2021YFQ0038"],"award-info":[{"award-number":["2021YFQ0038"]}],"id":[{"id":"10.13039\/501100004829","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004829","name":"Department of Science and Technology of Sichuan Province","doi-asserted-by":"publisher","award":["2022YFS0119"],"award-info":[{"award-number":["2022YFS0119"]}],"id":[{"id":"10.13039\/501100004829","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004829","name":"Department of Science and Technology of Sichuan Province","doi-asserted-by":"publisher","award":["2022YFS0286"],"award-info":[{"award-number":["2022YFS0286"]}],"id":[{"id":"10.13039\/501100004829","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004829","name":"Department of Science and Technology of Sichuan Province","doi-asserted-by":"publisher","award":["2023YFS0081"],"award-info":[{"award-number":["2023YFS0081"]}],"id":[{"id":"10.13039\/501100004829","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82001060"],"award-info":[{"award-number":["82001060"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2023,7]]},"abstract":"Abstract<\/jats:title>Aim<\/jats:title>Apical periodontitis is a prevalent oral inflammatory disease that has recently been linked to transcription factor EB (TFEB)\u2010mediated autophagy. Regulator of G\u2010protein signalling 10 (RGS10) is reported to be an effective regulator of the immune system and inflammation. This study aimed to investigate the involvement of RGS10 during the development of apical periodontitis through the TFEB\u2010mediated autophagy signalling pathway.<\/jats:p><\/jats:sec>Methodology<\/jats:title>Sixty BALB\/c mice were randomly divided into four groups of 15 mice for the in vivo<\/jats:italic> experiment. Rgs10 was locally overexpressed through eight injections of an adeno\u2010associated virus vector. The model of apical periodontitis was established 21\u2009days following pulp exposure, and the mice were euthanized to obtain mandibles for analysis. Micro\u2010computed tomography was employed to assess alveolar bone destruction, and the levels of Rgs10, TFEB\u2010mediated autophagy signalling factors and inflammatory factors were measured using quantitative reverse transcription polymerase chain reactions, western blotting, enzyme\u2010linked immunosorbent assays, immunofluorescence and immunohistochemistry. All experimental results were displayed as images or graphs. For the in vitro<\/jats:italic> experiments, we employed small interfering RNA (siRNA) to silence Rgs10<\/jats:italic> expression in RAW 264.7 cells. The data were analysed via one\u2010way anova<\/jats:sc> or Mann\u2013Whitney U<\/jats:italic> test\/Kruskal\u2013Wallis test of variance, where p<\/jats:italic>\u2009<\u2009.05 or U<\/jats:italic>\u2009>\u20091.96 was considered statistically significant.<\/jats:p><\/jats:sec>Results<\/jats:title>Local overexpression of Rgs10 reduced alveolar bone destruction within the apical periodontitis lesion and significantly decreased macrophage infiltration (p<\/jats:italic>\u2009<\u2009.05). Meanwhile, the expression of TFEB\u2010mediated autophagy signalling factors was upregulated, along with a decrease in inflammatory factor expression (p<\/jats:italic>\u2009<\u2009.05). Lipopolysaccharide\u2010stimulated RAW 264.7 cells exhibited decreased Rgs10 expression and TFEB\u2010mediated autophagy signalling. siRNA\u2010mediated silencing of Rgs10<\/jats:italic> further suppressed autophagy and concomitantly upregulated inflammatory factors (p<\/jats:italic>\u2009<\u2009.05).<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Collectively, the findings revealed that RGS10 suppresses the inflammatory response and bone destruction through TFEB\u2010mediated autophagy in apical periodontitis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.13924","type":"journal-article","created":{"date-parts":[[2023,4,24]],"date-time":"2023-04-24T13:34:54Z","timestamp":1682343294000},"page":"854-868","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["RGS10 negatively regulates apical periodontitis via TFEB\u2010mediated autophagy in BABL\/c mice model and in vitro<\/i>"],"prefix":"10.1111","volume":"56","author":[{"given":"Jiaxin","family":"Li","sequence":"first","affiliation":[{"name":"The State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, Department of Prosthodontics, West China Hospital of Stomatology Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Yue","sequence":"additional","affiliation":[{"name":"The State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, Department of Prosthodontics, West China Hospital of Stomatology Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weicheng","family":"Chan","sequence":"additional","affiliation":[{"name":"The State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, Department of Prosthodontics, West China Hospital of Stomatology Sichuan University Chengdu 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