{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T18:34:11Z","timestamp":1772044451419,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,5,23]],"date-time":"2023-05-23T00:00:00Z","timestamp":1684800000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Periapical granuloma (PG) and cyst (PC) are formed as a protective response consequent to pulpal infection leaching through the apical foramen and lateral canals. Various inflammatory mediators like mast cells and cyclooxygenase (COX)\u20102 are involved in this intricate process. This pilot study aimed to evaluate and compare the immunoexpression of tryptase and COX\u20102 in periapical granuloma and periapical cyst, and also correlate them with intensity of inflammatory infiltrate and thickness of cystic epithelial lining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>An observational and cross\u2010sectional study was conducted on paraffin\u2010embedded tissue sections of 50 PGs and 50 PCs submitted for morphological and immunohistochemical analysis using anti\u2010tryptase and anti\u2010COX\u20102 antibodies. The mean number of mast cells (total, granulated and degranulated), mean COX\u20102 expression and inflammatory score was calculated. The data obtained were analysed using Mann Whitney <jats:italic>U<\/jats:italic>, Student's <jats:italic>T<\/jats:italic>, Chi\u2010square and Spearman correlation test (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The inflammatory score, total mast cells and COX\u20102 expression were similar in PGs and PCs (<jats:italic>p<\/jats:italic>\u2009=\u2009.352, .339 and .352) however, the degranulated mast cells were highly significant in PC while granulated mast cells were highly significant in PG respectively (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001 in both). Although a non\u2010significant correlation existed between COX\u20102 and total mast cells in both groups but, total mast cells were significantly correlated with epithelial thickness in PC (<jats:italic>p<\/jats:italic>\u2009=\u2009.029).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Mast cells and cyclooxygenase\u20102 proved to be independent inflammatory markers in periapical lesions. Further studies should be planned on mast cell and COX\u20102 inhibitors as treatment modalities of periapical lesions.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.13931","type":"journal-article","created":{"date-parts":[[2023,5,12]],"date-time":"2023-05-12T13:50:41Z","timestamp":1683899441000},"page":"980-990","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Immunohistochemical evaluation of cyclooxygenase\u20102 and mast cell density in periapical lesions"],"prefix":"10.1111","volume":"56","author":[{"given":"Shashibala","family":"Malik","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology and Microbiology, Post Graduate Institute of Dental Sciences Pt B.D. Sharma University of Health Sciences  Rohtak India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5626-2622","authenticated-orcid":false,"given":"Mala","family":"Kamboj","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology and Microbiology, Post Graduate Institute of Dental Sciences Pt B.D. Sharma University of Health Sciences  Rohtak India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anjali","family":"Narwal","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology and Microbiology, Post Graduate Institute of Dental Sciences Pt B.D. Sharma University of Health Sciences  Rohtak India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anju","family":"Devi","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Pathology and Microbiology, Post Graduate Institute of Dental Sciences Pt B.D. 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