{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T16:30:36Z","timestamp":1770913836867,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,5,22]],"date-time":"2023-05-22T00:00:00Z","timestamp":1684713600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004779","name":"Pontificia Universidade Cat\u00f3lica do Paran\u00e1","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004779","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>To evaluate the expression of genes involved in chronic pain conditions in apical periodontitis (AP) tissues.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>An electronic search was performed in Scopus and MEDLINE (via PubMed) databases to identify articles (<jats:italic>n<\/jats:italic>\u2009=\u2009173) related to genes involved in chronic pain conditions. Full\u2010text reviews of the selected articles allowed the prioritization of 16 genes to be investigated with regards to their expression in AP tissues. Periapical lesions (<jats:italic>n<\/jats:italic>\u2009=\u200942) were collected during surgical endodontic procedures and processed for mRNA extraction and investigation of target genes via RT\u2013qPCR. Healthy periodontal ligament tissues obtained from third molar extractions were used as controls. Relative levels of target gene expression in AP and control tissues were normalized to <jats:italic>GAPDH<\/jats:italic> expression and compared using the 2<jats:sup>\u2212\u0394\u0394<jats:italic>C<\/jats:italic>t<\/jats:sup> method. Data analysis was performed using the Mann\u2013Whitney <jats:italic>U<\/jats:italic> test with a statistical significance threshold set at <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>mRNA expression levels of <jats:italic>MMP9<\/jats:italic>, <jats:italic>TIMP1<\/jats:italic>, <jats:italic>TNFA<\/jats:italic>, <jats:italic>CAMK4<\/jats:italic> and <jats:italic>COMT<\/jats:italic> were significantly increased in AP tissue samples compared with controls (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). Positive correlations were observed between the expression of <jats:italic>TIMP1<\/jats:italic> with <jats:italic>COMT<\/jats:italic> and <jats:italic>CAMK4<\/jats:italic>, <jats:italic>TNFA<\/jats:italic> with <jats:italic>TIMP1 and CAMK4<\/jats:italic>, <jats:italic>COMT<\/jats:italic> with <jats:italic>CAMK4<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study confirms the upregulation of <jats:italic>MMP9<\/jats:italic>, <jats:italic>TIMP1<\/jats:italic>, <jats:italic>TNFA<\/jats:italic> in AP tissues and reports for the first time, the expression of <jats:italic>CAMK4<\/jats:italic> and <jats:italic>COMT<\/jats:italic> as suggestive of their involvement in AP pathogenesis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.13932","type":"journal-article","created":{"date-parts":[[2023,5,15]],"date-time":"2023-05-15T04:44:51Z","timestamp":1684125891000},"page":"991-999","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Expression of chronic pain genes in apical periodontitis tissues"],"prefix":"10.1111","volume":"56","author":[{"given":"Jordano","family":"Francio","sequence":"first","affiliation":[{"name":"Department of Endodontics, School of Dentistry Pontifical Catholic University of Paran\u00e1  Curitiba Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Glaucia T.","family":"das Neves","sequence":"additional","affiliation":[{"name":"Department of Endodontics, School of Dentistry UTHealth School of Dentistry at Houston  Houston Texas USA"},{"name":"Center for Craniofacial Research UTHealth School of Dentistry at Houston  Houston Texas 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