{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T19:22:50Z","timestamp":1774898570451,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2023,8,16]],"date-time":"2023-08-16T00:00:00Z","timestamp":1692144000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF\u20102021R1A2C1004258"],"award-info":[{"award-number":["NRF\u20102021R1A2C1004258"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>The objective of this study was to assess and compare the cytocompatibility of decellularized porcine small intestine submucosal dural graft from Biodesign (BD) and polyester urethane\u2010based Neuro\u2010Patch (NP) dural substitute with the mineral trioxide aggregate (MTA) and the cyanoacrylate\u2010based Histoacryl surgical adhesive. Furthermore, the study evaluated the inflammatory response and osteogenic differentiation of human dental pulp stem cells (hDPSCs) when cultured in direct contact with the dural substitutes in comparison with MTA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>The viability of hDPSCs in direct contact with the tested materials was investigated <jats:italic>in vitro<\/jats:italic> by a CCK\u20108 assay. Additionally, the effects of dural substitutes and MTA on the expression of the inflammatory mediator interleukin\u20106 (IL\u20106) was investigated via enzyme\u2010linked immunosorbent assay (ELISA), whilst effects on the differentiation were evaluated using alizarin red staining, alkaline phosphatase staining, ELISA and energy\u2010dispersive X\u2010ray elemental mapping.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The dural substitutes were cytocompatible and promoted cellular adhesion. The Histoacryl and MTA demonstrated cytotoxicity in fresh preparations but showed a more favourable cellular reaction when set. Investigations of biological activity indicated that dural substitute membranes did not induce an inflammatory response or osteogenic differentiation of hDPSCs. In contrast, MTA induced the expression of IL\u20106 and alkaline phosphatase activity contributing to enhanced differentiation and mineralization.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The dural substitute membranes showed cytocompatibility, did not provoke an inflammatory response and maintained the stemness of hDPSCs better than MTA. Additionally, the set Histoacryl surgical adhesive demonstrated good biocompatibility. Taken together, these results highlight the potential use of dural substitutes in regenerative endodontic procedures as coronal barriers alternative to MTA to reduce the incidence of intracanal calcifications.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.13963","type":"journal-article","created":{"date-parts":[[2023,8,16]],"date-time":"2023-08-16T16:29:38Z","timestamp":1692203378000},"page":"1350-1359","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<i>In vitro<\/i> response of dental pulp stem cells to dural substitute grafts: Analysis of cytocompatibility and bioactivity"],"prefix":"10.1111","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1675-091X","authenticated-orcid":false,"given":"Ahmed","family":"Elhakim","sequence":"first","affiliation":[{"name":"Department of Conservative Dentistry and Oral Science Research Center, College of Dentistry Yonsei University  Seoul South Korea"},{"name":"Department of Endodontics, Faculty of Dentistry Mansoura University  Mansoura Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8889-9844","authenticated-orcid":false,"given":"Sunil","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Conservative Dentistry and Oral Science Research Center, College of Dentistry Yonsei University  Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Su\u2010Jung","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Conservative Dentistry and Oral Science Research Center Gangnam Severance Hospital, Yonsei University College of Dentistry  Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,8,16]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0027083"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00381\u2010012\u20101744\u20106"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3171\/jns.2007.106.6.1028"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s003290050136"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.inat.2020.100800"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fdmed.2020.00009"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dental.2004.05.010"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102591.2004.00859.x"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23052826"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102591.2011.01978.x"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2008.10.019"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0142\u20109612(94)90199\u20106"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/iej.12751"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.23288"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/iej.12030"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004770\u2010200312000\u201000007"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2008.05.013"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/347649"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910208100806"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0142\u20109612(92)90051\u2010o"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1177\/002203458506400410"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/2365104"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijom.2010.03.008"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.15274\/NRJ\u20102014\u201010052"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/iej.13542"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1155\/2009\/464280"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1006\/jsre.1997.5148"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673\u20105374.266043"},{"issue":"2","key":"e_1_2_9_30_1","first-page":"83","article-title":"Use of polyester urethane (Neuro\u2010Patch) as a dural substitute. 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