{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T13:23:39Z","timestamp":1790169819657,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T00:00:00Z","timestamp":1696291200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Limiting the incidence of resorption associated with delayed replantation of avulsed teeth is critical for long\u2010term tooth survival. In this study, we assessed whether icariin, a natural product with anti\u2010osteoclastic properties, could reduce root resorption in a rat model of tooth replantation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>Cytocompatibility of icariin (10, 20, 40 and 80\u2009\u03bcM) was evaluated by CCK\u20108 proliferation assay <jats:italic>in vitro<\/jats:italic>, and an osteoclastogenesis assay was performed to evaluate the effect of icariin on the differentiation of rat bone marrow macrophages and human peripheral blood monocytes into tartrate\u2010resistant acid phosphatase\u2010stained (TRAP<jats:sup>+<\/jats:sup>) multinucleated giant cells (MNGCs). Differentiation of human periodontal ligament stem cells (hPDLSCs) treated with icariin (10\u2009\u03bcM) was also evaluated at 5, 10 and 21\u2009days of osteogenic induction. The first maxillary molars of five\u2010week\u2010old male Sprague\u2013Dawley rats were extracted, denuded of PDL, then treated either with neutralized collagen solution (Carrier control) or icariin in collagen (3\u2009\u03bcg\/\u03bcL) before replantation into their sockets. The animals were euthanized 2\u2009weeks post\u2010surgery for micro\u2010computed tomography (micro\u2010CT) imaging and histological analyses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Icariin was cytocompatible and significantly reduced the differentiation of TRAP<jats:sup>+<\/jats:sup> MNGCs in a dose\u2010dependent manner compared to the control. Moreover, icariin enhanced alkaline phosphatase activity, expression of osteogenic marker genes and proteins, and calcium deposition in hPDLSCs. Micro\u2010CT imaging of the replanted samples demonstrated a significantly higher volume of remaining roots in the icariin\u2010treated group than in the control group. Histological analysis revealed a marked number of resorptive lacunae with TRAP activity in the control group, whereas icariin\u2010treated samples showed signs of functional healing and reduced osteoclastic activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Icariin was biocompatible and demonstrated potent anti\u2010osteoclastic and pro\u2010osteogenic properties that reduced resorption and promoted functional healing of denuded roots in a rat maxillary first molar model of replantation. These findings indicate that root surface treatment with icariin may be a clinically relevant and practical method for improving the retention and survival of teeth with compromised PDL after delayed replantation following traumatic avulsion.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.13980","type":"journal-article","created":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T08:40:34Z","timestamp":1696408834000},"page":"1550-1558","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Effect of icariin surface treatment on the resorption of denuded roots after replantation in rat"],"prefix":"10.1111","volume":"56","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1675-091X","authenticated-orcid":false,"given":"Ahmed","family":"Elhakim","sequence":"first","affiliation":[{"name":"Microscope Center, Department of Conservative Dentistry and Oral Science Research Center Yonsei University College of Dentistry  Seoul 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