{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T03:05:00Z","timestamp":1786763100797,"version":"build-2736575974"},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,12,5]],"date-time":"2023-12-05T00:00:00Z","timestamp":1701734400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81971902"],"award-info":[{"award-number":["81971902"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82173458"],"award-info":[{"award-number":["82173458"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Guanylate\u2010binding protein 5 (GBP5) is an interferon (IFN)\u2010inducible GTPase that plays a crucial role in the cell\u2010autonomous immune response against microbial infections. In this study, we investigated the immunoregulatory role of GBP5 in the pathogenesis of dental pulpitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>Gene\u2010set enrichment analysis (GSEA) was utilized to evaluate the IFN\u2010\u03b3 signalling pathway, and the differential expression of GBP mRNA in normal versus inflamed dental pulp tissues was screened, based on Gene Expression Omnibus (GEO) datasets associated with pulpitis. Both normal pulp tissues and inflamed pulp tissues were used for experiments. The expression of IFNs and GBPs was determined by qRT\u2013PCR. Immunoblotting and double immunofluorescence were performed to examine the cellular localization of GBP5 in dental pulp tissues. For the functional studies, IFN\u2010\u03b3 priming or lentivirus vector\u2010delivered shRNA was used to, respectively, overexpress or knock down endogenous GBP5 expression in human dental pulp stem cells (HDPSCs). Subsequently, LPS was used to stimulate HDPSCs (overexpressing or with knocked\u2010down GBP5) to establish an in\u00a0vitro model of inflammation. qRT\u2013PCR and ELISA were employed to examine the expression of proinflammatory cytokines (IL\u20106, IL\u20108 and IL\u20101\u03b2) and cyclooxygenase 2 (COX2). Every experiment has three times of biological replicates and three technical replicates, respectively. Statistical analysis was performed using the Student's <jats:italic>t<\/jats:italic>\u2010test and one\u2010way <jats:sc>ANOVA<\/jats:sc>, and a <jats:italic>p<\/jats:italic>\u2010value of &lt;.05 was considered statistically significant.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>GSEA analysis based on the GEO dataset revealed a significant activation of the IFN\u2010\u03b3 signalling pathway in the human pulpitis group. Among the human GBPs evaluated, GBP5 was selectively upregulated in inflamed dental pulp tissues and predominantly expressed in dental pulp cells. In\u00a0vitro experiments demonstrated that IFN\u2010\u03b3 robustly induced the expression of GBP5 in HDPSCs. Knockdown of GBP5 expression in HDPSCs significantly amplified the LPS\u2010induced upregulation of inflammatory mediators (IL\u20106, IL\u20108, IL\u20101\u03b2 and COX2) both with and without IFN\u2010\u03b3 priming.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our findings demonstrated that GBP5 partook in the pathogenesis of dental pulpitis. The involvement of GBP5 in pulpitis appeared to coordinate the regulation of inflammatory cytokines. Knockdown of GBP5 contributed to the exacerbation of LPS\u2010mediated inflammation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.14006","type":"journal-article","created":{"date-parts":[[2023,12,5]],"date-time":"2023-12-05T08:40:15Z","timestamp":1701765615000},"page":"208-218","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Guanylate\u2010binding protein 5\u2010mediated cell\u2010autonomous immunity suppresses inflammation in dental pulpitis: An\u00a0in\u00a0vitro study"],"prefix":"10.1111","volume":"57","author":[{"given":"Minchun","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Stomatology, Nanfang Hospital Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaoning","family":"Zhan","sequence":"additional","affiliation":[{"name":"Department of Stomatology, Nanfang Hospital Southern Medical University  Guangzhou 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