{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T06:30:40Z","timestamp":1788330640413,"version":"build-2803163510"},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,12,18]],"date-time":"2023-12-18T00:00:00Z","timestamp":1702857600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>This study evaluated the transdentinal cytotoxic effects of enzymatic agents (EA) for chemomechanical carious tissue removal on human dental pulp cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>The groups were based on the performed dentine treatments (<jats:italic>n<\/jats:italic>\u2009=\u20098): G1: Positive Control (PC \u2010 no treatment); G2: Negative Control (NC \u2010 35% H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> for 2\u2009min); G3: Brix 3000\u2122 (BX) for 30\u2009s; G4: BX for 2\u2009min; G5: Papacarie Duo\u2122 (PD) for 30\u2009s; G6: PD for 2\u2009min. The cells were evaluated for viability (VB; MTT assay) and production of reactive oxygen species (ROS; DCFH\u2010DA assay) and nitric oxide (NO; Griess reagent). A scanning electron microscope provided morphological chemical analyses and energy\u2010dispersive X\u2010ray spectroscopy. The data were submitted to the one\u2010way <jats:sc>anova<\/jats:sc> statistical test complemented by Tukey (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Cell viability decreased by 21.1% and 58.4% in G5 and G6, respectively. ROS production in G3 and G4 maintained basal levels but increased by 171.2% and 75.1% in G5 and G6, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The Brix3000\u2122 enzymatic agent did not cause indirect cytotoxic effects on pulp cells, regardless of the application time. Conversely, Papacarie Duo\u2122 reduced viability and increased ROS production by pulp cells.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.14013","type":"journal-article","created":{"date-parts":[[2023,12,18]],"date-time":"2023-12-18T14:53:57Z","timestamp":1702911237000},"page":"315-327","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Viability and oxidative stress of dental pulp cells after indirect application of chemomechanical agents: An in\u00a0vitro study"],"prefix":"10.1111","volume":"57","author":[{"given":"Caio Luiz","family":"Lins\u2010Candeiro","sequence":"first","affiliation":[{"name":"Post\u2010graduation Program in Dentistry, School of Dentistry Federal University of Uberl\u00e2ndia  Uberl\u00e2ndia Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz Renato","family":"Paranhos","sequence":"additional","affiliation":[{"name":"Division of Preventive and Community 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