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In specific groups (WT+Pam3cys and TLR2KO+Pam3cys), the synthetic ligand Pam3cys was administered intraperitoneally every 7\u2009days, according to the experimental period (14, 21 and 42\u2009days). At the end of those periods, the animals were euthanized, and the mandible and the spleen were submitted to histotechnical processing. Mandible histological sections were analysed by haematoxylin and eosin, TRAP histoenzymology and immunohistochemistry (FOXP3, RANK, RANKL and OPG). Spleen sections were analysed by immunohistochemistry (FOXP3).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The inflammatory infiltrate and bone resorption were more intense in the TLR2KO group compared to the WT group. The animals that received the Pam3cys had smaller periapical lesions when compared to the animals that did not receive the ligand (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). TLR2KO animals showed a significant increase in the number of osteoclasts when compared to TLR2KO+Pam3cys group (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). At 21\u2009days, the WT+Pam3cys group had a lower number of osteoclasts when compared to the WT animals (<jats:italic>p<\/jats:italic>\u2009=\u2009.02). FOXP3 expression was more intense in the WT+Pam3cys groups when compared to the WT animals in the 42\u2009days (<jats:italic>p<\/jats:italic>\u2009=\u2009.03). In the spleen analysis, the WT+Pam3cys group also had a higher expression of FOXP3 when compared to the WT animals at 14 and 42\u2009days (<jats:italic>p<\/jats:italic>\u2009=\u2009.02). Concerning RANKL, there was a reduction in staining in the KOTLR2+Pam3cys groups at 21 and 42\u2009days (<jats:italic>p<\/jats:italic>\u2009=\u2009.03) and a higher binding ratio between RANK\/RANKL in animals that did not receive the ligand.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Administration of the Pam3cys increased the proliferation of Tregs, showed by FOXP3 expression and prevented the progression of the periapical lesion in WT mice. On the other hand, in the TLR2KO animals, Treg expression was lower with larger areas of periapical lesions. Finally, systemic administration of the Pam3cys in KO animals was able to limit the deleterious effects of the absence of the TLR2 receptor.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.14015","type":"journal-article","created":{"date-parts":[[2024,1,18]],"date-time":"2024-01-18T15:57:10Z","timestamp":1705593430000},"page":"328-343","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<scp>TLR2<\/scp> agonist prevents the progression of periapical lesions in mice by reducing osteoclast activity and regulating the frequency of Tregs"],"prefix":"10.1111","volume":"57","author":[{"given":"Lana Kei Yamamoto","family":"Almeida","sequence":"first","affiliation":[{"name":"Department of Pediatric Dentistry School of Dentistry of Ribeir\u00e3o Preto, University of S\u00e3o Paulo  Ribeir\u00e3o Preto SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo 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