{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T12:49:56Z","timestamp":1791377396538,"version":"4.3.2"},"reference-count":58,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,5,4]],"date-time":"2024-05-04T00:00:00Z","timestamp":1714780800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001412","name":"Council of Scientific and Industrial Research, India","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001412","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>The success of vital pulp treatment (VPT) procedures is dependent on an accurate diagnosis of the pulpal inflammatory condition. Compared with current subjective pulpal diagnostic tests, inflammatory molecular biomarkers involved in the pathogenesis of pulpitis represent potential objective indicators of the degree of pulpal inflammation. Therefore, the aim of this study was to quantify level of inflammatory biomarkers \u2013 Interleukin 8 (IL\u20108) and TNF\u2010\u03b1 in patients diagnosed with reversible pulpitis (RP), irreversible pulpitis (IR) and normal pulp (NP) and investigate their diagnostic accuracy in differentiating between healthy and inflamed conditions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methodology<\/jats:title>\n                    <jats:p>\n                      This prospective, cross\u2010sectional study enrolled 72 patients aged 14\u201353\u2009years with extremely deep carious lesions after establishing a clinical diagnosis of RP (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200942), symptomatic IR (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200922) and NP (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20098). 50\u2009\u03bcL of pulpal blood sample was collected from all the patients using a micropipette after pulpal exposure. The level of IL\u20108 and TNF\u2010\u03b1 was assessed in pg\/mL using enzyme\u2010linked immunosorbent assays. Mann\u2013Whitney\n                      <jats:italic>U<\/jats:italic>\n                      test was applied to establish the association between IL\u20108\/TNF\u2010\u03b1 level and degree of pulp inflammation. Receiver operating curve (ROC) analysis was carried out to calculate area under the curve (AUC) for RP versus IR. Cut\u2010off values were established using Youden's index.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      IL\u20108 and TNF\u2010\u03b1 levels differed significantly between RP and IR groups (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009\u2264\u2009.001). The median value of IL\u20108 in RP and IP groups was 259.8\u2009pg\/mL [187.5\u2013310.0] and 1357.8\u2009pg\/mL [1036.7\u20132177.6] respectively. The AUC\u2010ROC curve for RP versus IR was 0.997 with 95.5% sensitivity and 99.76% specificity. The median value of TNF\u2010\u03b1 in RP and IR groups was 75.4\u2009pg\/mL [62.7\u201395.8] and 157.6\u2009pg\/mL [94.1\u2013347.3]. The AUC\u2010ROC curve for TNF\u2010\u03b1 was 0.812 with a sensitivity and specificity of 59.1% and 92.1%, respectively. IL\u20108 and TNF\u2010\u03b1 levels were below detection levels for all NP samples.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study showed that pulpal blood could provide an excellent medium for establishing pulpal diagnosis under extremely deep carious lesions. The selected cytokines, IL\u20108 and TNF\u2010\u03b1, demonstrated excellent discriminatory performance for reversible and irreversible pulpitis. Future studies should correlate the IL\u20108\/TNF\u2010\u03b1 levels with VPT treatment outcomes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/iej.14078","type":"journal-article","created":{"date-parts":[[2024,5,4]],"date-time":"2024-05-04T07:24:23Z","timestamp":1714807463000},"page":"1200-1211","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Discriminatory performance of the pulpal inflammatory biomarkers; Interleukin\u20108 and\n                    <scp>TNF<\/scp>\n                    \u2010\u03b1 in patients with symptoms indicative of reversible and irreversible pulpitis: A diagnostic accuracy study"],"prefix":"10.1111","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7264-8506","authenticated-orcid":false,"given":"Ruchika Roongta","family":"Nawal","sequence":"first","affiliation":[{"name":"Department of Conservative Dentistry &amp; Endodontics Maulana Azad Institute of Dental Sciences  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5656-4628","authenticated-orcid":false,"given":"Sudha","family":"Yadav","sequence":"additional","affiliation":[{"name":"Department of Conservative Dentistry &amp; Endodontics Maulana Azad Institute of Dental Sciences  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8690-2379","authenticated-orcid":false,"given":"Henry Fergus","family":"Duncan","sequence":"additional","affiliation":[{"name":"Division of Restorative Dentistry Dublin Dental University Hospital, Trinity College Dublin  Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6805-4695","authenticated-orcid":false,"given":"Sangeeta","family":"Talwar","sequence":"additional","affiliation":[{"name":"Department of Conservative Dentistry &amp; Endodontics Maulana Azad Institute of Dental Sciences  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6347-6247","authenticated-orcid":false,"given":"Aishvarya","family":"Kaushik","sequence":"additional","affiliation":[{"name":"Department of Conservative Dentistry &amp; Endodontics Maulana Azad Institute of Dental Sciences  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7299-4660","authenticated-orcid":false,"given":"Vijay K.","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Maulana Azad Medical College and Associated Hospitals  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0447-0176","authenticated-orcid":false,"given":"Bidhan C.","family":"Koner","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Maulana Azad Medical College and Associated Hospitals  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,5,4]]},"reference":[{"key":"e_1_2_15_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2012.12.035"},{"key":"e_1_2_15_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2011.10.005"},{"key":"e_1_2_15_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/1306396"},{"key":"e_1_2_15_5_1","unstructured":"American Association of Endodontists. (2013)Endodontic diagnosis. Available from:https:\/\/www.aae.org\/specialty\/wpcontent\/uploads\/sites\/2\/2017\/07\/endodonticdiagnosisfall2013.pdf[Accessed 4th April 2022]"},{"key":"e_1_2_15_6_1","first-page":"4629","article-title":"Inhibitors of prostaglandin synthesis inhibit human prostate tumor cell invasiveness and reduce the release of matrix metalloproteinases","volume":"60","author":"Attiga F.A.","year":"2000","journal-title":"Cancer Research"},{"key":"e_1_2_15_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(92)80909-Z"},{"key":"e_1_2_15_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm9082408"},{"key":"e_1_2_15_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2005.12.008"},{"key":"e_1_2_15_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2591.2006.01149.x"},{"key":"e_1_2_15_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-662-52901-0_4"},{"key":"e_1_2_15_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/iej.13343"},{"key":"e_1_2_15_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/iej.12225"},{"key":"e_1_2_15_14_1","doi-asserted-by":"publisher","DOI":"10.1155\/2009\/365785"},{"key":"e_1_2_15_15_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2016-012799"},{"key":"e_1_2_15_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2591.1980.tb00834.x"},{"key":"e_1_2_15_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2014.07.001"},{"key":"e_1_2_15_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/iej.12030"},{"key":"e_1_2_15_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/iej.13080"},{"key":"e_1_2_15_20_1","first-page":"191","article-title":"Concentration of collagenases (MMP\u20101, \u20108, \u201013) in patients with chronically inflamed dental pulp tissue","volume":"33","author":"Evrosimovska B.","year":"2012","journal-title":"Prilozi"},{"key":"e_1_2_15_21_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/230251"},{"key":"e_1_2_15_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2010.08.006"},{"key":"e_1_2_15_23_1","first-page":"63","article-title":"Detection of interleukin\u20108 in exudates from normal and inflamed human dental pulp tissues","volume":"3","author":"Guo X.","year":"2000","journal-title":"The Chinese Journal of Dental Research"},{"key":"e_1_2_15_24_1","doi-asserted-by":"publisher","DOI":"10.11613\/BM.2016.034"},{"key":"e_1_2_15_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2007.01.001"},{"key":"e_1_2_15_26_1","doi-asserted-by":"publisher","DOI":"10.14219\/jada.archive.1970.0293"},{"key":"e_1_2_15_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1079-2104(99)70118-6"},{"key":"e_1_2_15_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/iej.13872"},{"key":"e_1_2_15_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/iej.13950"},{"key":"e_1_2_15_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2019.154896"},{"key":"e_1_2_15_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2591.2007.01215.x"},{"key":"e_1_2_15_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26144129"},{"key":"e_1_2_15_33_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0909-8836.1999.eos107209.x"},{"key":"e_1_2_15_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2019.05.003"},{"key":"e_1_2_15_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2591.2012.02016.x"},{"key":"e_1_2_15_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2015.10.020"},{"key":"e_1_2_15_37_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2591.2002.00579.x"},{"key":"e_1_2_15_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxu102"},{"key":"e_1_2_15_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0099-2399(06)80438-3"},{"issue":"2","key":"e_1_2_15_40_1","first-page":"108","article-title":"Reliability of the periapical index scoring system","volume":"96","author":"Orstavik D.","year":"1988","journal-title":"Scandinavian Journal of Dental Research"},{"key":"e_1_2_15_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0188-4409(02)00396-X"},{"key":"e_1_2_15_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/0030-4220(66)90143-5"},{"key":"e_1_2_15_43_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.CCM.0000186783.34908.18"},{"key":"e_1_2_15_44_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/9259646"},{"key":"e_1_2_15_45_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.5.3476"},{"key":"e_1_2_15_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/0030-4220(63)90323-2"},{"issue":"1","key":"e_1_2_15_47_1","first-page":"129","article-title":"Effect of neuropeptides on human dental pulp cells; mRNA expression of proinflammatory cytokines","volume":"45","author":"Shida M.","year":"2011","journal-title":"Journal of Osaka Dental University"},{"key":"e_1_2_15_48_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1678-77572009000500031"},{"key":"e_1_2_15_49_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2008.061010"},{"key":"e_1_2_15_50_1","doi-asserted-by":"publisher","DOI":"10.2298\/VSP140303172S"},{"key":"e_1_2_15_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2591.2011.01986.x"},{"key":"e_1_2_15_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1198"},{"key":"e_1_2_15_53_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-263X.2002.00321.x"},{"key":"e_1_2_15_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/iej.12793"},{"key":"e_1_2_15_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/iej.12339"},{"key":"e_1_2_15_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2012.04.005"},{"key":"e_1_2_15_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2017.02.009"},{"key":"e_1_2_15_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1043-4666(03)00116-9"},{"key":"e_1_2_15_59_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/39.4.561"}],"container-title":["International Endodontic Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iej.14078","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,19]],"date-time":"2024-09-19T01:03:47Z","timestamp":1726707827000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/iej.14078"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,4]]},"references-count":58,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2024,9]]}},"alternative-id":["10.1111\/iej.14078"],"URL":"https:\/\/doi.org\/10.1111\/iej.14078","archive":["Portico"],"relation":{},"ISSN":["0143-2885","1365-2591"],"issn-type":[{"value":"0143-2885","type":"print"},{"value":"1365-2591","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,4]]},"assertion":[{"value":"2023-10-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}