{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:15:33Z","timestamp":1789517733636,"version":"build-2803163510"},"reference-count":85,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T00:00:00Z","timestamp":1734566400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002873","name":"Chulalongkorn University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002873","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>\n                      Cannabidiol (CBD), derived from the\n                      <jats:italic>Cannabis sativa<\/jats:italic>\n                      plant, exhibits benefits in potentially alleviating a number of oral and dental pathoses, including pulpitis and periodontal diseases. This study aimed to explore the impact of CBD on several traits of human dental pulp stem cells (hDPSC), such as their proliferation, apoptosis, migration and odonto\/osteogenic differentiation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methodology<\/jats:title>\n                    <jats:p>\n                      hDPSCs were harvested from human dental pulp tissues. The cells were treated with CBD at concentrations of 1.25, 2.5, 5, 10, 25 and 50\u2009\u03bcg\/mL. Cell responses in terms of cell proliferation, colony\u2010forming unit, cell cycle progression, cell migration, apoptosis and odonto\/osteogenic differentiation of hDPSCs were assessed in the normal culture condition and\n                      <jats:italic>P. gingivalis<\/jats:italic>\n                      lipopolysaccharide (LPS)\u2010induced \u2018inflammatory\u2019 milieus. RNA sequencing and proteomic analysis were performed to predict target pathways impacted by CBD.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>CBD minimally affects hDPSCs' behaviour under normal culture growth milieu in normal conditions. However, an optimal concentration of 1.25\u2009\u03bcg\/mL CBD significantly countered the harmful effects of LPS, indicated by the promoting cell proliferation and restoring the odonto\/osteogenic differentiation potential of hDPSCs under LPS\u2010treated conditions. The proteomic analysis demonstrated that several proteins involved in cell proliferation and differentiation were upregulated following CBD exposure, including CCL8, CDC42 and KFL5. RNA sequencing data indicated that CBD upregulated the Notch signalling pathway. In an inhibitory experiment, DAPT, a Notch inhibitor, reduced the effect of CBD\u2010rescued LPS\u2010attenuated mineralization in hDPSCs, suggesting that CBD potentially mediates Notch activation to exert its impact on odonto\/osteogenic differentiation of hDPSCs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>CBD recovers the proliferation and survival of hDPSCs following exposure to LPS. Additionally, we report that CBD\u2010mediated Notch activation effectively restores the odonto\/osteogenic differentiation ability of hDPSCs under inflamed conditions. These results underscore the potential role of CBD as a therapeutic option to enhance dentine regeneration.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/iej.14183","type":"journal-article","created":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T03:33:44Z","timestamp":1734579224000},"page":"449-466","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Cannabidiol alleviates\n                    <scp>LPS<\/scp>\n                    \u2010inhibited odonto\/osteogenic differentiation in human dental pulp stem cells in\u00a0vitro"],"prefix":"10.1111","volume":"58","author":[{"given":"Chatvadee","family":"Kornsuthisopon","sequence":"first","affiliation":[{"name":"Department of Anatomy, Faculty of Dentistry Chulalongkorn University  Bangkok Thailand"},{"name":"Center of Excellence for Dental Stem Cell Biology, Faculty of Dentistry Chulalongkorn University  Bangkok 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