{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T02:34:09Z","timestamp":1789871649627,"version":"4.0.1"},"reference-count":43,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,3,14]],"date-time":"2025-03-14T00:00:00Z","timestamp":1741910400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>This study aimed to compare the release of inflammatory mediators and post\u2010operative pain after conservative and conventional root canal preparations of mandibular molars with asymptomatic irreversible pulpitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methodology<\/jats:title><jats:p>Forty\u2010five healthy patients diagnosed with asymptomatic irreversible pulpitis were randomly assigned to three groups based on the system used for root canal preparation (<jats:italic>n<\/jats:italic>\u2009=\u200915): ProTaper Gold, OneShape and TruNatomy. Gingival crevicular fluid samples were collected for baseline measurements, 24 and 72\u2009h after root canal treatment. Inflammatory mediators (Substance P, IL\u20106, IL\u201010 and PGE\u20102) were quantified using enzyme\u2010linked immunosorbent assays, and post\u2010operative pain was assessed using the visual analogue scale (VAS) and compared among groups with linear regression analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All mediators exhibited an increase at 24\u2009h and a decrease at 72\u2009h. The release of Substance P in the OneShape group was significantly higher than in the TruNatomy and ProTaper Gold groups (Odds Ratio (OR)\u2009=\u200917.4 and 21.7, respectively, at 24\u2009h and 21.5 and 15.6 at 72\u2009h; <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). IL\u20106 and IL\u201010 were significantly higher in the OneShape and ProTaper Gold groups compared to TruNatomy at 24\u2009h (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). PGE2 levels were not affected by the type of instruments (<jats:italic>p<\/jats:italic>\u2009&gt;\u2009.05). VAS scores were significantly higher at 24\u2009h compared to baseline, with root canal preparation using OneShape significantly associated with higher post\u2010operative pain than preparation with ProTaper Gold (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). Analgesic intake was not related to the instrumentation group or any other patient\u2010 or tooth\u2010related factors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Conservative root canal preparation of mandibular molars with asymptomatic irreversible pulpitis decreased the release of inflammatory mediators but did not influence post\u2010operative pain.<\/jats:p><\/jats:sec>","DOI":"10.1111\/iej.14224","type":"journal-article","created":{"date-parts":[[2025,3,14]],"date-time":"2025-03-14T15:58:11Z","timestamp":1741967891000},"page":"862-872","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Impact of conservative versus conventional instrumentation on the release of inflammatory mediators and post\u2010operative pain in mandibular molars with asymptomatic irreversible pulpitis: A randomized clinical trial"],"prefix":"10.1111","volume":"58","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9513-0841","authenticated-orcid":false,"given":"S\u0131la Nur","family":"Usta","sequence":"first","affiliation":[{"name":"Department of Endodontics, Gulhane Faculty of Dentistry University of Health Sciences  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2270-8096","authenticated-orcid":false,"given":"Ana","family":"Arias","sequence":"additional","affiliation":[{"name":"Department of Conservative and Prosthetic Dentistry, School of Dentistry Complutense University  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emre","family":"Avc\u0131","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmacy University of Health Sciences  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6445-8243","authenticated-orcid":false,"given":"Emmanuel Jo\u00e3o Nogueira Leal","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Endodontics, School of Dentistry Grande Rio University (UNIGRANRIO)  Rio de Janeiro Brazil"},{"name":"Department of Integrated Clinical Procedures, School of Dentistry Rio de Janeiro State University (UERJ)  Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,14]]},"reference":[{"key":"e_1_2_13_2_1","unstructured":"AAE. 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