{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T16:56:10Z","timestamp":1773334570208,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:00:00Z","timestamp":1767830400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:00:00Z","timestamp":1767830400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>To evaluate the cleaning efficacy and biological effects of ultrasonic (US)\u2010activated sodium hypochlorite (NaOCl) for root surface decontamination and decellularisation in delayed tooth replantation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methodology<\/jats:title>\n                    <jats:p>Cementum discs prepared from extracted healthy single\u2010rooted teeth were treated with varying NaOCl concentrations (1%\u20135.25%), with or without US activation. Necrotic periodontal ligament (PDL) tissue clearance, cementum integrity and elemental composition were inspected by scanning electron microscopy and energy dispersive X\u2010ray spectroscopy. Biocompatibility of the treated cementum discs with human periodontal ligament cells (PDLCs) and rat bone marrow mesenchymal stem cells was assessed, alongside osteogenic differentiation, inflammatory cytokine expression, collagen type I (COL\u2010I) and receptor activator of nuclear factor\u2010\u03baB ligand (RANKL)\/osteoprotegerin (OPG) signalling. A Sprague\u2013Dawley rat maxillary incisor delayed replantation model was established to assess in\u00a0vivo healing outcomes of US\u2010activated NaOCl, evaluated by micro\u2010computed tomography, haematoxylin and eosin staining and Masson's trichrome staining and tartrate\u2010resistant acid phosphatase (TRAP) histochemistry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Increasing NaOCl concentration accelerated removal of necrotic PDL. US activation significantly shortened NaOCl treatment time (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05) and allowed effective necrotic PDL removal at 1% NaOCl without damaging cementum. Treated cementum surfaces showed higher calcium\/phosphorus and lower carbon\/nitrogen contents (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01). US\u2010assisted 1% NaOCl enhanced cell adhesion, osteogenic differentiation and COL\u2010I expression while reducing interleukin\u20106, tumour necrosis factor\u2010\u03b1 and the RANKL\/OPG ratio. In\u00a0vivo, NaOCl combined with US activation significantly reduced root resorption volume (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05), preserved periodontal architecture, and decreased TRAP\u2010positive osteoclast counts compared with saline or untreated controls.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>US\u2010activated 1% NaOCl achieves rapid, selective removal of necrotic periodontal tissue while preserving cementum and producing a decellularised surface. This strategy attenuates inflammatory responses, suppresses osteoclastogenesis, mitigates inflammatory root resorption and may extend the therapeutic window for delayed tooth replantation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/iej.70093","type":"journal-article","created":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T11:29:22Z","timestamp":1767871762000},"page":"680-693","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Impacts of Ultrasonic Activated Sodium Hypochlorite on Delayed Replantation of Avulsed Tooth: Cleaning Efficiency and Healing Effectiveness"],"prefix":"10.1111","volume":"59","author":[{"given":"Shasha","family":"He","sequence":"first","affiliation":[{"name":"Department of General Dentistry, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology Nanjing University  Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of General Dentistry, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology Nanjing University  Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qizheng","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of General Dentistry, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology Nanjing University  Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuying","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of General Dentistry, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology Nanjing University  Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Han","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of General Dentistry, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology Nanjing University  Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yijia","family":"Cao","sequence":"additional","affiliation":[{"name":"School of Stomatology Xuzhou Medical University  Xuzhou Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1760-4443","authenticated-orcid":false,"given":"Gang","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Stomatology, Jinling Hospital, Affiliated Hospital of Medical School Nanjing University  Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2184-9970","authenticated-orcid":false,"given":"Ting","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of General Dentistry, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology Nanjing University  Nanjing Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,8]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/edt.12762"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-9657.2012.01125.x"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/iej.12467"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.4103\/JCDE.JCDE_158_25"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/pho.2023.0193"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2022.121786"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/edt.12308"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2019.11.013"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-020-03430-1"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.4155\/fsoa-2016-0016"},{"key":"e_1_2_11_12_1","first-page":"75","article-title":"Assessment of the Effects of Citric Acid and EDTA on Cell Viability of Cultured Human Periodontal Ligament Cells Attached to Simulated Avulsed Permanent Tooth Using a Spectrofluorometer\u2010An in Vitro Study","volume":"7","author":"Doiphode A. 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