{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T00:06:04Z","timestamp":1789171564447,"version":"build-2803163510"},"reference-count":62,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:00:00Z","timestamp":1782172800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:00:00Z","timestamp":1782172800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100001424","name":"American Liver Foundation","doi-asserted-by":"publisher","award":["RV\u2010967705"],"award-info":[{"award-number":["RV\u2010967705"]}],"id":[{"id":"10.13039\/100001424","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To characterize the intracanal microbiota and identify key microbial taxa and functional pathways associated with regenerative endodontic treatment (RET) outcomes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methodology<\/jats:title>\n                    <jats:p>In this observational cohort study, 196 samples were collected at five time points from external tooth surfaces (T1, T4) and root canals (T2, T3, T5). The V3\u2013V4 hypervariable region of 16S rRNA gene was amplified and sequenced on the Illumina MiSeq platform. Sequence data were pre\u2010processed using QIIME2 and DADA2 to generate amplicon sequence variants (ASVs), classified against the eHOMD database. Microbial diversity, differential abundance, and Receiver Operating Characteristic (ROC) analyses were performed using R statistical software. Functional profiles were predicted using PICRUSt2.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      RET induced dynamic shifts in the root canal microbiome, with initially predominant phyla including Actinomycetota, Bacillota, Bacteroidota, Fusobacteriota and Pseudomonadota. Cases with treatment failure consistently showed a higher relative abundance of Fusobacteriota than successful cases. Alpha diversity varied across time points, with outcome\u2010specific differences observed only at T3; beta diversity differed significantly by time point but not by treatment outcome. Differential abundance analysis at T5 revealed enrichment of\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Rothia dentocariosa<\/jats:italic>\n                      <\/jats:styled-content>\n                      and\n                      <jats:italic>Leptothrix<\/jats:italic>\n                      sp. in successful cases, whereas failed cases were enriched in\n                      <jats:italic>Fusobacterium polymorphum<\/jats:italic>\n                      ,\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Streptococcus sanguinis<\/jats:italic>\n                      <\/jats:styled-content>\n                      ,\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Campylobacter showae<\/jats:italic>\n                      <\/jats:styled-content>\n                      ,\n                      <jats:italic>Lachnoanaerobaculum<\/jats:italic>\n                      sp. and\n                      <jats:italic>Selenomonas<\/jats:italic>\n                      sp. These taxa demonstrated moderate diagnostic potential, with area under the curve (AUC) values of 0.70\u20130.82. Persistence analysis showed that\n                      <jats:italic>F. polymorphum<\/jats:italic>\n                      ,\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>C. showae<\/jats:italic>\n                      <\/jats:styled-content>\n                      and\n                      <jats:italic>Selenomonas<\/jats:italic>\n                      sp. exhibited greater persistence in failed cases across treatment time points. Predicted functional profiling indicated distinct metabolic potentials between successful and failed treatment groups.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>RET alters the intracanal microbiome, with taxa identified following intracanal dressing exhibiting moderate predictive potential as biomarkers for treatment outcome, thereby contributing to early prognosis and informing RET strategies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/iej.70205","type":"journal-article","created":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T11:43:46Z","timestamp":1782215026000},"page":"2223-2237","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Distinct Microbiota and Functional Pathway Profiles Define Success and Failure in Regenerative Endodontic Treatment"],"prefix":"10.1111","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2454-2862","authenticated-orcid":false,"given":"Leimarembi Devi","family":"Naorem","sequence":"first","affiliation":[{"name":"Department of Odontology Ume\u00e5 University  Ume\u00e5 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8281-9241","authenticated-orcid":false,"given":"Alina","family":"Wikstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Endodontics, Public Dental Health Services Eastman Institute  Stockholm Sweden"},{"name":"Division of Paediatric Dentistry, Department of Dental Medicine Karolinska Institute  Stockholm Sweden"},{"name":"Centre of Paediatric Oral Health  Huddinge Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Quach","sequence":"additional","affiliation":[{"name":"Region of V\u00e4sterbotten  Ume\u00e5 Sweden"},{"name":"Region of Uppsala  Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9751-5745","authenticated-orcid":false,"given":"Lokeshwaran","family":"Manoharan","sequence":"additional","affiliation":[{"name":"National Bioinformatics Infrastructure Sweden (NBIS) Lund University  Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9738-0828","authenticated-orcid":false,"given":"Ronald","family":"Ordinola\u2010Zapata","sequence":"additional","affiliation":[{"name":"Division of Endodontics, Department of Restorative Sciences, School of Dentistry University of Minnesota  Minneapolis Minnesota USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3536-4467","authenticated-orcid":false,"given":"Olena","family":"Rakhimova","sequence":"additional","affiliation":[{"name":"Department of Odontology Ume\u00e5 University  Ume\u00e5 Sweden"},{"name":"Department of Medical Biosciences Ume\u00e5 University  Ume\u00e5 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8747-3307","authenticated-orcid":false,"given":"Malin","family":"Brundin","sequence":"additional","affiliation":[{"name":"Department of Odontology Ume\u00e5 University  Ume\u00e5 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5674-8179","authenticated-orcid":false,"given":"Nelly Romani","family":"Vestman","sequence":"additional","affiliation":[{"name":"Department of Odontology Ume\u00e5 University  Ume\u00e5 Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,23]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2019.02.004"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0049576"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781118445112.stat07841"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010019\u20100209\u20109"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3869"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/database\/baq013"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2009.090185"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2017.7300"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.13348"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.4103\/jomfp.JOMFP_304_18"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics9080433"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1654\u20101103.2003.tb02228.x"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010020\u20100548\u20106"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034511405388"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/edt.12494"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2022.07.004"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/iej.12629"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2007.05.017"},{"key":"e_1_2_10_20_1","doi-asserted-by":"crossref","unstructured":"Halko N. P.\u2010G.Martinsson Y.Shkolnisky andM.Tygert.2011.\u201cAn Algorithm for the Principal Component Analysis of Large Data Sets.\u201d arXiv:1007.5510. Preprint arXiv March 19.https:\/\/doi.org\/10.48550\/arXiv.1007.5510.","DOI":"10.1137\/100804139"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034513487559"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2008.03.001"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0193504"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2008.10.004"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000659"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bdj.2016.865"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/iej.12954"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1080\/01621459.1952.10483441"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines11102749"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034510370026"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010020\u201017041\u20107"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1009442"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.14806\/ej.17.1.200"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0061217"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102591.2011.01886.x"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096\u2010019\u201003641\u20109"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.4103\/0972\u20100707.73386"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12503"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.02811"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102105\u201012\u201077"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.22037\/iej.v17i3.32703"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201069931\u2010x"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.555465"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1128\/msystems.00170\u201022"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2012.03.021"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253\u2010025\u201013476\u20104"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwp039"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb\u20102011\u201012\u20106\u2010r60"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.3389\/fdmed.2023.1281337"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12903\u2010022\u201002562\u2010y"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bdj.4809477"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.3402\/jom.v4i0.10743"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/iej.14071"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12934\u2010018\u20100880\u20104"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601\u20101546.2003.00039.x"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.202100443"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI154944"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens14010012"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1111\/iej.13735"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/edt.13062"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1080\/20002297.2024.2343518"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2008.07.005"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btad470"}],"container-title":["International Endodontic Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iej.70205","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/iej.70205","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iej.70205","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T23:12:01Z","timestamp":1789168321000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/iej.70205"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,23]]},"references-count":62,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/iej.70205"],"URL":"https:\/\/doi.org\/10.1111\/iej.70205","archive":["Portico"],"relation":{},"ISSN":["0143-2885","1365-2591"],"issn-type":[{"value":"0143-2885","type":"print"},{"value":"1365-2591","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,23]]},"assertion":[{"value":"2026-02-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}