{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T18:44:03Z","timestamp":1792176243427,"version":"4.4.0"},"reference-count":46,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T00:00:00Z","timestamp":1784678400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>This study investigated the long\u2010term survival of bacteria in root canals obturated using two endodontic sealers\u2014AH Plus Jet (AHP) and BioRoot RCS (BCS)\u2014while also assessing the influence of ultrasonic agitation on their antimicrobial effectiveness.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methodology<\/jats:title>\n                    <jats:p>\n                      One hundred human single\u2010rooted teeth were contaminated for 21\u2009days with dual\u2010species biofilms of\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Enterococcus faecalis<\/jats:italic>\n                      <\/jats:styled-content>\n                      and\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Streptococcus oralis<\/jats:italic>\n                      <\/jats:styled-content>\n                      . Samples were collected at various treatment stages: initial (S1), post\u2010instrumentation (S2), and after filling removal (S3), with additional samples after 7\u2009days (S4). Groups were categorized by sealer type, incubation duration (30\u2009days and 12\u2009months), and ultrasonic agitation (UA): AHP, AHP\/UA, BCS, BCS\/UA, and a control group (CON) without a sealer. Bacterial survival was assessed through colony\u2010forming unit (CFU) counts, and viability was visualized via confocal laser scanning microscopy (CLSM).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Bacterial counts immediately after root filling removal (S3) remained comparable to those observed before obturation (S2) but significantly increased after 7\u2009days of nutritional exposure (S4) in all groups (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05). CLSM corroborated the CFU findings, indicating that bacteria remained viable within dentinal tubules after 30\u2009days and 12\u2009months. The use of UA enhanced the antimicrobial effectiveness of the sealers during the 30\u2010day period (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05). After 12\u2009months, AHP exhibited lower bacterial growth, particularly for\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>E. faecalis<\/jats:italic>\n                      <\/jats:styled-content>\n                      , compared with BCS (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Ultrasonic agitation reduced the ability of residual bacteria to recolonize the root canal space during the initial 30\u2010day period. However, viable bacteria persisted even after 12\u2009months of obturation, irrespective of the sealer and obturation technique used.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/iej.70227","type":"journal-article","created":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T07:18:00Z","timestamp":1784704680000},"page":"2424-2435","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Exploring the Impact of Ultrasonic Activation on the Antimicrobial Activity of Root Canal Sealers: A Year\u2010Long Ex\u00a0Vivo Investigation Into Bacterial Survival"],"prefix":"10.1111","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6762-0242","authenticated-orcid":false,"given":"F. L. P.","family":"Viana","sequence":"first","affiliation":[{"name":"Post\u2010Graduate Program in Dentistry Federal University of Cear\u00e1  Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-0874-4890","authenticated-orcid":false,"given":"M. A. F.","family":"Damasceno","sequence":"additional","affiliation":[{"name":"Post\u2010Graduate Program in Dentistry Federal University of Cear\u00e1  Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8735-065X","authenticated-orcid":false,"given":"M. P.","family":"Alcalde","sequence":"additional","affiliation":[{"name":"Department of Dentistry, Endodontics and Dental Materials, Bauru Dental School University of S\u00e3o Paulo (USP)  Bauru SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0419-5699","authenticated-orcid":false,"given":"R. R.","family":"Vivan","sequence":"additional","affiliation":[{"name":"Department of Dentistry, Endodontics and Dental Materials, Bauru Dental School University of S\u00e3o Paulo (USP)  Bauru SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7246-5171","authenticated-orcid":false,"given":"I. G.","family":"de Moraes","sequence":"additional","affiliation":[{"name":"Department of Dentistry, Endodontics and Dental Materials, Bauru Dental School University of S\u00e3o Paulo (USP)  Bauru SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3051-737X","authenticated-orcid":false,"given":"M. A. H.","family":"Duarte","sequence":"additional","affiliation":[{"name":"Department of Dentistry, Endodontics and Dental Materials, Bauru Dental School University of S\u00e3o Paulo (USP)  Bauru SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7499-4031","authenticated-orcid":false,"given":"E. T.","family":"Pinheiro","sequence":"additional","affiliation":[{"name":"Department of Dentistry, S\u00e3o Paulo School of Dentistry University of S\u00e3o Paulo (USP)  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4415-5680","authenticated-orcid":false,"given":"B. C.","family":"Vasconcelos","sequence":"additional","affiliation":[{"name":"Post\u2010Graduate Program in Dentistry Federal University of Cear\u00e1  Fortaleza Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,22]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1590\/1678-7757-2017-0090"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.job.2023.09.004"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2016.08.001"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10266-019-00425-7"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdent.2016.06.008"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdent.2015.05.003"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.4103\/JCD.JCD_64_18"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2591.2011.01992.x"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm9092722"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2015.04.011"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/aej.12769"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2022.03.008"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2013.11.016"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2020.12.004"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dental.2019.08.105"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10266-023-00888-9"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2017.08.023"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dental.2020.11.011"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2591.2005.00981.x"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/ma17081932"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/ma12030531"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0109-5641(03)00067-8"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdent.2020.103283"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2591.2001.00437.x"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.12.5157-5164.1997"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/ma16206705"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/iej.13542"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2591.2006.01099.x"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2022.01.016"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10266-024-00901-9"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2591.2001.00373.x"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2016.09.018"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0143-2885.2004.00785.x"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2007.01.010"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2591.2005.01009.x"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/ma12152482"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/iej.12856"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2016.11.019"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1590\/0103-6440202205100"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2008.07.028"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-016-1960-7"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1079-2104(98)90404-8"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.4317\/jced.62477"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2013.09.026"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/iej.12513"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1590\/1807-3107BOR-2016.vol30.0018"}],"container-title":["International Endodontic Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iej.70227","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/iej.70227","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iej.70227","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T17:46:34Z","timestamp":1792172794000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/iej.70227"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,22]]},"references-count":46,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2026,11]]}},"alternative-id":["10.1111\/iej.70227"],"URL":"https:\/\/doi.org\/10.1111\/iej.70227","archive":["Portico"],"relation":{},"ISSN":["0143-2885","1365-2591"],"issn-type":[{"value":"0143-2885","type":"print"},{"value":"1365-2591","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,22]]},"assertion":[{"value":"2026-02-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}