{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T08:05:01Z","timestamp":1790841901506,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2024\/16085\u20100"],"award-info":[{"award-number":["2024\/16085\u20100"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2024\/16074\u20108"],"award-info":[{"award-number":["2024\/16074\u20108"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2022\/08004\u20104"],"award-info":[{"award-number":["2022\/08004\u20104"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2021\/02070\u20102"],"award-info":[{"award-number":["2021\/02070\u20102"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["Finance Code 001"],"award-info":[{"award-number":["Finance Code 001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To investigate the effects of overtraining on the development of apical periodontitis (AP) in mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methodology<\/jats:title>\n                    <jats:p>Sixty 6\u2010week\u2010old male C57BL\/6 mice were allocated to six groups: sedentary without AP (SED); sedentary with AP (SED AP); aerobic training without AP (AERO); aerobic training with AP (AERO AP); overtraining without AP (OVER); and overtraining with AP (OVER AP). The exercise protocols lasted 8\u2009weeks, with AP induced in the fourth week by coronal opening of the lower first molars. At the end of the experiment, physical performance was assessed, together with periradicular bone resorption by micro\u2010computed tomography, inflammatory infiltrate density, the number of tartrate\u2010resistant acid phosphatase\u2010positive (TRAP+) osteoclasts and immunostaining for IL\u20106, TNF\u2010\u03b1 and IL\u201010. Gene expression of cytokines and markers related to bone resorption (IL\u20101\u03b2, IL\u20106, TNF\u2010\u03b1, RANKL, osteoprotegerin and cathepsin K) was analysed by RT\u2010qPCR, and plasma levels of IL\u20101\u03b2, IL\u20106, IL\u201010 and TNF\u2010\u03b1 were determined by multiplex immunoassay.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The OVER and OVER AP groups showed a significant reduction in physical performance, supporting the induction of a chronic excessive exercise\/non\u2010functional overreaching\u2010like state (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05). OVER AP group exhibited a higher volume of periradicular bone resorption compared with the AERO AP and SED AP groups (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05). Furthermore, the OVER AP group showed more intense inflammatory infiltrate, a higher number of TRAP+ cells and greater immunostaining for IL\u20106 and TNF\u2010\u03b1 than the AERO AP group (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05), but it did not differ from the SED AP group (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&gt;\u20090.05). Gene expression of inflammatory and osteoclastogenic markers was higher in groups with AP than in those without AP (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05), whereas no significant differences were observed among the different exercise intensities (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&gt;\u20090.05). Osteoprotegerin expression showed no significant differences among the groups (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&gt;\u20090.05). Analysis of plasma cytokine levels showed no effect of overtraining (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&gt;\u20090.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Chronic excessive exercise aggravated AP, resulting in greater periradicular bone resorption and loss of the protective adaptations associated with moderate aerobic exercise. Overtrained animals displayed an inflammatory and osteoclastic profile comparable to that of sedentary animals, but with a more severe periapical bone destruction pattern.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/iej.70278","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:45:43Z","timestamp":1790840743000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Chronic Excessive Exercise Aggravates Apical Periodontitis in Mice"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-4930-1707","authenticated-orcid":false,"given":"Andr\u00e9ia Bosco","family":"Boaventura","sequence":"first","affiliation":[{"name":"Araraquara School of Dentistry, Department of Restorative Dentistry S\u00e3o Paulo State University (UNESP)  Araraquara S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosmeli Daysi","family":"Coasaca\u2010Rivera","sequence":"additional","affiliation":[{"name":"Araraquara School of Dentistry, Department of Restorative Dentistry S\u00e3o Paulo State University (UNESP)  Araraquara S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7694-4732","authenticated-orcid":false,"given":"B\u00e1rbara Roma","family":"Mendes","sequence":"additional","affiliation":[{"name":"Araraquara School of Dentistry, Department of Restorative Dentistry S\u00e3o Paulo State University (UNESP)  Araraquara S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9473-3739","authenticated-orcid":false,"given":"Sandra Lia","family":"do Amaral","sequence":"additional","affiliation":[{"name":"Department of Physical Education S\u00e3o Paulo State University (UNESP)  Bauru S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9679-8357","authenticated-orcid":false,"given":"Adelino Sanchez Ramos","family":"da Silva","sequence":"additional","affiliation":[{"name":"University of S\u00e3o Paulo (USP), School of Physical Education and Sport of Ribeir\u00e3o Preto, Postgraduate Program in Rehabilitation and Functional Performance, Ribeir\u00e3o Preto Medical School  Ribeir\u00e3o Preto S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5756-5828","authenticated-orcid":false,"given":"Paulo Sergio","family":"Cerri","sequence":"additional","affiliation":[{"name":"Araraquara School of Dentistry, Department of Morphology, Genetics, Orthodontics and Pediatric Dentistry S\u00e3o Paulo State University (UNESP)  Araraquara S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7030-3718","authenticated-orcid":false,"given":"Gisele","family":"Faria","sequence":"additional","affiliation":[{"name":"Araraquara School of Dentistry, Department of Restorative Dentistry S\u00e3o Paulo State University (UNESP)  Araraquara S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,1]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2023.07.013"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnetp.2021.794392"},{"key":"e_1_2_11_4_1","doi-asserted-by":"crossref","first-page":"29","DOI":"10.54646\/bijrdpm.2023.05","article-title":"Risks of Overtraining and Compulsive Exercising on Human Health","volume":"1","author":"Bereda G.","year":"2023","journal-title":"BOHR International Journal of Current Research in Diabetes and Preventive Medicine"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034520952341"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10266-021-00636-x"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1155\/ijod\/7317709"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2009.04.013"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmet.2025.102135"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1681.2007.04635.x"},{"issue":"1","key":"e_1_2_11_11_1","doi-asserted-by":"crossref","first-page":"8030","DOI":"10.1038\/s41598-024-58384-1","article-title":"Physical Training Attenuates Systemic Cytokine Response and Tissue Damage Triggered by Apical Periodontitis","volume":"14","author":"Ferreira R. 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