{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T05:45:49Z","timestamp":1791783949227,"version":"4.3.4"},"reference-count":45,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T00:00:00Z","timestamp":1791676800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T00:00:00Z","timestamp":1791676800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To overcome the confounding effects of taxonomic heterogeneity in primary apical periodontitis (AP), this study aimed to characterize the quantitative and compositional profiles of the root canal microbiota associated with severe clinical features (percussion tenderness, large periapical lesions, and sinus tracts) using integrated relative (RMP) and quantitative microbiome profiling (QMP).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methodology<\/jats:title>\n                    <jats:p>Root canal samples from 52 patients (out of 62 initially enrolled) with primary AP were analysed using spike\u2010in\u2013based 16S rRNA sequencing. Microbial load, diversity, and taxonomic composition were evaluated across these three predefined clinical features (with large lesions defined as \u2265\u20095\u2009mm). Spearman correlation analysis was applied to assess the associations between differentially abundant genera and clinical severity. Furthermore, PICRUSt2 was used to infer functional profiles and calculate taxonomic contributions to enriched pathways under both RMP and QMP frameworks.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Samples with percussion tenderness exhibited significantly elevated microbial loads, with similar trends observed for large periapical lesions and sinus tracts. Percussion\u2010positive samples also showed significantly higher Inverse Simpson index and Pielou's evenness index, whereas no significant differences in alpha diversity were observed for large periapical lesions or sinus tracts. Beta diversity did not differ significantly across any of the three clinical features under either QMP or RMP. Spearman correlation analysis identified a core bacterial group\u2014Porphyromonas, Treponema, Tannerella, Peptostreptococcus, Selenomonas, Fusobacterium, and Fretibacterium\u2014whose members showed broadly positive associations with all three clinical features in both relative and absolute abundance. Functional inference revealed these taxa collectively accounted for a shared set of virulence\u2010associated pathways (e.g., lipopolysaccharide\/peptidoglycan biosynthesis and protein export) consistently enriched in severe cases.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Despite taxonomic heterogeneity in the root canal microbiota, severe clinical features of primary AP were associated with elevated microbial load and with increased relative and absolute abundances of a candidate bacterial group, whose members converged on a shared set of virulence\u2010associated pathways, suggesting that compositionally diverse communities may nevertheless converge on common virulence\u2010related functions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/iej.70289","type":"journal-article","created":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T05:07:36Z","timestamp":1791781656000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Quantitative and Relative Microbiome Profiling Reveals a Bacterial Group Associated With Primary Apical Periodontitis Severity and Sharing a Predicted Virulence\u2010Associated Functional Signature"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6274-7674","authenticated-orcid":false,"given":"Yefei","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Endodontics, Shanghai Stomatological Hospital and School of Stomatology Fudan University  Shanghai China"},{"name":"Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufeng","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Endodontics, Shanghai Stomatological Hospital and School of Stomatology Fudan University  Shanghai China"},{"name":"Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mi","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Endodontics, Shanghai Stomatological Hospital and School of Stomatology Fudan University  Shanghai China"},{"name":"Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3736-779X","authenticated-orcid":false,"given":"Zhifei","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Endodontics, Shanghai Stomatological Hospital and School of Stomatology Fudan University  Shanghai China"},{"name":"Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Endodontics, Shanghai Stomatological Hospital and School of Stomatology Fudan University  Shanghai China"},{"name":"Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Endodontics, Shanghai Stomatological Hospital and School of Stomatology Fudan University  Shanghai China"},{"name":"Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7327-457X","authenticated-orcid":false,"given":"Xiaoling","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Endodontics, Shanghai Stomatological Hospital and School of Stomatology Fudan University  Shanghai China"},{"name":"Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,11]]},"reference":[{"issue":"11","key":"e_1_2_13_2_1","doi-asserted-by":"crossref","DOI":"10.1007\/s00784-024-05946-2","article-title":"Association Between Endodontic Symptoms and Root Canal Microbiota: A Systematic Review and Meta\u2010Analysis of Bacteroidetes, Spirochaetes and Fusobacteriales","volume":"28","author":"Aboushadi M. 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