{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T09:49:20Z","timestamp":1791798560771,"version":"4.3.4"},"reference-count":45,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:00:00Z","timestamp":1791763200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:00:00Z","timestamp":1791763200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int Endodontic J"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To quantitatively compare the bacterial DNA load in root apices and periapical lesions surgically retrieved from teeth with failed root canal treatment, and to investigate the association between bacterial DNA load in both anatomical locations and the three\u2010dimensional (3D) volumetric extent of periapical bone loss, as well as relevant clinical and demographic variables.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methodology<\/jats:title>\n                    <jats:p>\n                      Root apices and periapical lesions were surgically obtained from patients with failed root canal treatments. Samples were cryopulverized and bacterial DNA was quantified by q\u2010PCR assay. The volume of the periapical lesions was calculated by cone\u2010beam computed tomography (CBCT) images segmentation using Blue Sky Bio software. Demographic and clinical data including gender, age, previous retreatment, tenderness to percussion, tooth position, and periapical healing after 1\u2009year were preliminarily associated with the lesion volume. Normality of the variables was analyzed by the Shapiro Wilk test, and global comparisons were explored by ANOVA when variances were similar. The Student\n                      <jats:italic>t<\/jats:italic>\n                      test was used for pair\u2010by\u2010pair comparisons.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Seventy\u2010nine samples (40 root apices and 39 lesions) were successfully amplified. A greater number of DNA copies was found in apices as compared with apical lesions (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05). Analysis by lesion volume showed that a higher bacterial load in apices was associated with larger apical lesions, as well as in lesions of patients under 50\u2009years old (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05). Clinical parameters such as tooth position and patient age showed an association with the volume of apical lesions (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Root apices harbour higher bacterial DNA than periapical lesions, regardless of the volume of apical bone loss. Apices associated with large volume lesions present a greater amount of bacterial DNA. Preliminary associations were identified between certain clinical and demographic factors (age and tooth position) and increased lesion volume.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/iej.70293","type":"journal-article","created":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T09:26:36Z","timestamp":1791797196000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Association between bacterial load and volumetric bone loss in teeth with persistent apical periodontitis: A cone\u2010beam computed tomography segmentation\u2010based observational study"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7778-8738","authenticated-orcid":false,"given":"Carmen","family":"Solana","sequence":"first","affiliation":[{"name":"Department of Stomatology, School of Dentistry University of Granada  Granada Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria ibs.GRANADA  Granada Spain"},{"name":"Private Practice  Andalucia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-8048-8834","authenticated-orcid":false,"given":"Adela","family":"Baca","sequence":"additional","affiliation":[{"name":"Department of Stomatology, School of Dentistry University of Granada  Granada Spain"},{"name":"Private Practice  Andalucia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4233-8969","authenticated-orcid":false,"given":"Virginia","family":"P\u00e9rez\u2010Carrasco","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n Biosanitaria ibs.GRANADA  Granada Spain"},{"name":"GENYO. Centre for Genomics and Oncological Research: Pfizer\/University of Granada\/Andalusian Regional Government, PTS Granada  Spain"},{"name":"Microbiology Unit University Hospital Virgen de las Nieves  Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2249-6724","authenticated-orcid":false,"given":"David","family":"Uroz\u2010Torres","sequence":"additional","affiliation":[{"name":"Private Practice  Andalucia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6107-5853","authenticated-orcid":false,"given":"Carmen Mar\u00eda","family":"Ferrer\u2010Luque","sequence":"additional","affiliation":[{"name":"Department of Stomatology, School of Dentistry University of Granada  Granada Spain"},{"name":"Instituto de Investigaci\u00f3n Biosanitaria ibs.GRANADA  Granada 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