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The aim of this study was to evaluate gluconeogenesis from alanine, pyruvate and glycerol, and related metabolic parameters in perfused liver from <jats:styled-content style=\"fixed-case\">W<\/jats:styled-content>alker\u2010256 tumour\u2010bearing rats on days 5 (<jats:styled-content style=\"fixed-case\">WK<\/jats:styled-content>5 group), 8 (<jats:styled-content style=\"fixed-case\">WK<\/jats:styled-content>8 group) and 12 (<jats:styled-content style=\"fixed-case\">WK<\/jats:styled-content>12 group) of tumour development. There was reduction (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.05) of liver glucose production from alanine and pyruvate in <jats:styled-content style=\"fixed-case\">WK<\/jats:styled-content>5, <jats:styled-content style=\"fixed-case\">WK<\/jats:styled-content>8 and <jats:styled-content style=\"fixed-case\">WK<\/jats:styled-content>12 groups, which was accompanied by a decrease (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.05) in oxygen consumption. Moreover, there was higher (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.05) pyruvate and lactate production from alanine in the <jats:styled-content style=\"fixed-case\">WK<\/jats:styled-content>5 group and a marked reduction (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.05) of pyruvate and urea production from alanine in the <jats:styled-content style=\"fixed-case\">WK<\/jats:styled-content>12 group. In addition, liver glucose production and oxygen consumption from glycerol were not reduced in <jats:styled-content style=\"fixed-case\">WK<\/jats:styled-content>5, <jats:styled-content style=\"fixed-case\">WK<\/jats:styled-content>8 and <jats:styled-content style=\"fixed-case\">WK<\/jats:styled-content>12 groups. Thus the, the results show inhibition of hepatic gluconeogenesis from alanine and pyruvate, but not from glycerol, on days 5, 8 and 12 of Walker\u2010256 tumour development, which can be attributed to the metabolic step in which the substrate enters the gluconeogenic pathway.<\/jats:p>","DOI":"10.1111\/iep.12002","type":"journal-article","created":{"date-parts":[[2013,1,15]],"date-time":"2013-01-15T03:40:34Z","timestamp":1358221234000},"page":"47-55","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Changes in liver gluconeogenesis during the development of Walker\u2010256 tumour in rats"],"prefix":"10.1111","volume":"94","author":[{"given":"Carolina Campos Lima","family":"Moreira","sequence":"first","affiliation":[{"name":"Department of Physiological Sciences State University of Londrina Londrina Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Priscila","family":"Cassolla","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences State University of Londrina Londrina Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Paula Segantini","family":"Dornellas","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences State University of Londrina Londrina Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hely","family":"de Morais","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences State University of Londrina Londrina Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camila Oliveira","family":"de Souza","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences State University of Londrina Londrina Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Glaucia Regina","family":"Borba\u2010Murad","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences State University of Londrina Londrina Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto Barbosa","family":"Bazotte","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Therapeutics State University of Maring\u00e1 Maring\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helenir Medri","family":"de Souza","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences State University of Londrina Londrina Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-7843.2007.00124.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0899-9007(00)00407-X"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.1444"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/1049909108315518"},{"key":"e_1_2_7_6_1","first-page":"1205","volume-title":"Methods of Enzymatic Analysis","author":"Bergmeyer H.U.","year":"1974"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1006\/jsre.1993.1185"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mehy.2006.04.048"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.1163"},{"key":"e_1_2_7_10_1","first-page":"275","volume-title":"M\u00e9todos de Laborat\u00f3rio em Bioqu\u00edmica","author":"Bracht A.","year":"2003"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(98)01485-9"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1043-4666(03)00098-X"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/JES.0b013e3181f44f11"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.2792"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2009.02.024"},{"key":"e_1_2_7_16_1","first-page":"187","article-title":"The urea cycle and related pathways in the liver of Walker\u2010256 tumor\u2010bearing rats","volume":"1688","author":"Corbello\u2010Pereira S.R.","year":"2004","journal-title":"Biochem. 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