{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T14:44:27Z","timestamp":1782139467025,"version":"3.54.5"},"reference-count":37,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,9,2]],"date-time":"2013-09-02T00:00:00Z","timestamp":1378080000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Experimental Path"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Bronchiolar <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>lara cells play a critical role in lung homoeostasis. The main goal of this study was to evaluate the effects of chronic allergy on these cells and the efficacy of budesonide (<jats:styled-content style=\"fixed-case\">BUD<\/jats:styled-content>) and montelukast (<jats:styled-content style=\"fixed-case\">MK<\/jats:styled-content>) in restoring their typical phenotypes after ovalbumin\u2010induced chronic allergy in mice. Chronic allergy induced extensive bronchiolar Alcian blue\u2010periodic acid\u2010Schiff (AB\/PAS)\u2010positive metaplasia. In addition, cells accumulated numerous big electron\u2010lucent granules negative for <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>lara cell main secretory protein (<jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content>16), and consequently, <jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content>16 was significantly reduced in bronchoalveolar lavage. A concomitant reduction in <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">CYP<\/jats:styled-content>2<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>1 content was observed. The phenotypic changes induced by allergy were pharmacologically reversed by both treatments; MK was more efficient than BUD in doing so. MK decreased <jats:styled-content style=\"fixed-case\">AB<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">PAS<\/jats:styled-content> reactivity to control levels whereas they remained persistently elevated after <jats:styled-content style=\"fixed-case\">BUD<\/jats:styled-content>. Moreover, most non\u2010ciliated cells recovered their normal morphology after <jats:styled-content style=\"fixed-case\">MK<\/jats:styled-content>, whereas for <jats:styled-content style=\"fixed-case\">BUD<\/jats:styled-content> normal cells coexisted with \u2018transitional\u2019 cells that contained remnant mucous granules and stained strongly for <jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content>16 and <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>. Glucocorticoids were also less able to reduce inflammatory infiltration and maintained higher percentage of neutrophils, which may have contributed to prolonged mucin expression. These results show that chronic allergy\u2010induced mucous metaplasia of <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>lara cells affects their defensive mechanisms. However, anti\u2010inflammatory treatments were able to re\u2010establish the normal phenotype of <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>lara cell, with <jats:styled-content style=\"fixed-case\">MK<\/jats:styled-content> being more efficient at restoring a normal profile than <jats:styled-content style=\"fixed-case\">BUD<\/jats:styled-content>. This study highlights the role of epithelial cells in lung injuries and their contribution to anti\u2010inflammatory therapies.<\/jats:p>","DOI":"10.1111\/iep.12041","type":"journal-article","created":{"date-parts":[[2013,9,3]],"date-time":"2013-09-03T05:19:06Z","timestamp":1378185546000},"page":"399-411","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Restoration of the normal <scp>C<\/scp>lara cell phenotype after chronic allergic inflammation"],"prefix":"10.1111","volume":"94","author":[{"given":"Felix D.","family":"Roth","sequence":"first","affiliation":[{"name":"Centro de Microscop\u00eda Electr\u00f3nica INICSA\u2010CONICET Facultad de Ciencias M\u00e9dicas Universidad Nacional de C\u00f3rdoba C\u00f3rdoba Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amado A.","family":"Quintar","sequence":"additional","affiliation":[{"name":"Centro de Microscop\u00eda Electr\u00f3nica INICSA\u2010CONICET Facultad de Ciencias M\u00e9dicas Universidad Nacional de C\u00f3rdoba C\u00f3rdoba Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Leimgruber","sequence":"additional","affiliation":[{"name":"Centro de Microscop\u00eda Electr\u00f3nica INICSA\u2010CONICET Facultad de Ciencias M\u00e9dicas Universidad Nacional de C\u00f3rdoba C\u00f3rdoba Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Centro de Microscop\u00eda Electr\u00f3nica INICSA\u2010CONICET Facultad de Ciencias M\u00e9dicas Universidad Nacional de C\u00f3rdoba C\u00f3rdoba Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa M.","family":"Uribe Echevarr\u00eda","sequence":"additional","affiliation":[{"name":"Centro de Microscop\u00eda Electr\u00f3nica INICSA\u2010CONICET Facultad de Ciencias M\u00e9dicas Universidad Nacional de C\u00f3rdoba C\u00f3rdoba Argentina"},{"name":"Sanatorio Allende C\u00f3rdoba Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alicia I.","family":"Torres","sequence":"additional","affiliation":[{"name":"Centro de Microscop\u00eda Electr\u00f3nica INICSA\u2010CONICET Facultad de Ciencias M\u00e9dicas Universidad Nacional de C\u00f3rdoba C\u00f3rdoba Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina A.","family":"Maldonado","sequence":"additional","affiliation":[{"name":"Centro de Microscop\u00eda Electr\u00f3nica INICSA\u2010CONICET Facultad de Ciencias M\u00e9dicas Universidad Nacional de C\u00f3rdoba C\u00f3rdoba Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,9,2]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.03.00098803"},{"key":"e_1_2_6_3_1","first-page":"243","article-title":"Early events of experimental exposure to amorphous and crystalline silica in the rat: time course of surfactant protein d","volume":"107","author":"Barbaro M.","year":"2002","journal-title":"Ital. 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