{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T08:16:04Z","timestamp":1740125764549,"version":"3.37.3"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,4,29]],"date-time":"2014-04-29T00:00:00Z","timestamp":1398729600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["30772277","81170334"],"award-info":[{"award-number":["30772277","81170334"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Experimental Path"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Hirschsprung's disease (<jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content>) is a developmental disorder of the enteric nervous system characterized by aganglionosis in distal gut. In this study, we used two\u2010dimensional gel electrophoresis (2\u2010<jats:styled-content style=\"fixed-case\">DE<\/jats:styled-content>) technology coupled with matrix assisted laser desorption\/ionization time\u2010of\u2010flight mass spectrometry (<jats:styled-content style=\"fixed-case\">MALDI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TOF<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>) analysis to identify differentially expressed proteins in the aganglionic (stenotic) and ganglionic (normal) colon segment tissues from patients with <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content>. We identified 15 proteins with different expression levels between the stenotic and the normal colon segment tissues from patients with <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content>. Nine proteins were upregulated and six proteins downregulated in the stenotic colon segment tissues compared to the normal colon segment tissues. Based on the biological functions, we selected the <jats:styled-content style=\"fixed-case\">Hsp<\/jats:styled-content>27 upregulated proteins and the <jats:styled-content style=\"fixed-case\">PRDX<\/jats:styled-content>3 downregulated proteins to confirm their expression in 20 patients. The protein and <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expressions of <jats:styled-content style=\"fixed-case\">Hsp<\/jats:styled-content>27 were statistically higher in the stenotic colon segment tissues than in the normal colon segment tissues, whereas the protein and <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expressions of <jats:styled-content style=\"fixed-case\">PRDX<\/jats:styled-content>3 were statistically lower in the stenotic colon segment tissues than in the normal colon segment tissues. These findings of changes in mRNA and protein in tissues from patients with HSCR provide information which may be helpful in understanding the pathomechanism that is implicated in the disease.<\/jats:p>","DOI":"10.1111\/iep.12075","type":"journal-article","created":{"date-parts":[[2014,4,29]],"date-time":"2014-04-29T04:56:42Z","timestamp":1398747402000},"page":"229-237","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Comparative study of <scp>Hsp<\/scp>27, <scp>GSK<\/scp>3\u03b2, <scp>Wnt<\/scp>1 and <scp>PRDX<\/scp>3 in Hirschsprung's disease"],"prefix":"10.1111","volume":"95","author":[{"given":"Hong","family":"Gao","sequence":"first","affiliation":[{"name":"Laboratory of Pediatric Congenital Malformation Ministry of Public Health Shengjing Hospital of China Medical University Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaomei","family":"Liu","sequence":"additional","affiliation":[{"name":"Laboratory of Pediatric Congenital Malformation Ministry of Public Health Shengjing Hospital of China Medical University Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong","family":"Chen","sequence":"additional","affiliation":[{"name":"Laboratory of Pediatric Congenital Malformation Ministry of Public Health Shengjing Hospital of China Medical University Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liangying","family":"Lv","sequence":"additional","affiliation":[{"name":"Laboratory of Pediatric Congenital Malformation Ministry of Public Health Shengjing Hospital of China Medical University Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei","family":"Wu","sequence":"additional","affiliation":[{"name":"Laboratory of Pediatric Congenital Malformation Ministry of Public Health Shengjing Hospital of China Medical University Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Mi","sequence":"additional","affiliation":[{"name":"Laboratory of Pediatric Congenital Malformation Ministry of Public Health Shengjing Hospital of China Medical University Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weilin","family":"Wang","sequence":"additional","affiliation":[{"name":"Laboratory of Pediatric Congenital Malformation Ministry of Public Health Shengjing Hospital of China Medical University Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,4,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.38.11.729"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.11.7583"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200701135"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.60.070191.001541"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/thy.2005.15.205"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.12.066"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-0082(01)00011-9"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.sempedsurg.2008.07.005"},{"key":"e_1_2_8_10_1","first-page":"1743","article-title":"Overexpression and abnormal modification of the stress proteins alpha B\u2010crystallin and HSP27 in Alexander disease","volume":"143","author":"Head M.W.","year":"1993","journal-title":"Am. 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