{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T06:33:13Z","timestamp":1776493993697,"version":"3.51.2"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,4,25]],"date-time":"2014-04-25T00:00:00Z","timestamp":1398384000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"crossref","award":["5\/4\/3-2\/2009-NCD-II"],"award-info":[{"award-number":["5\/4\/3-2\/2009-NCD-II"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Experimental Path"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Pancreatic acinar cell necrosis is indicative of severe pancreatitis and the degree of necrosis is an index of its outcome. We studied whether the dose and duration of injury correlates with severity, particularly in terms of necrosis, in caerulein\u2010induced acute pancreatitis (<jats:styled-content style=\"fixed-case\">AP<\/jats:styled-content>) in <jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>wiss albino mice. In addition to control group 1 (<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>1), groups 2 and 3 received four injections of caerulein every hour but were sacrificed at five hours (<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>2) and nine hours (<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>3) respectively, and group 4 received eight injections and was sacrificed at nine hours (<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>4). The severity of pancreatitis was assessed histopathologically and biochemically. The histopathological scores of pancreatitis in groups 3 and 4 were significantly higher than in groups 1 and 2 (4 <jats:italic>vs<\/jats:italic>. 1, 4 <jats:italic>vs<\/jats:italic>. 2, 3 <jats:italic>vs<\/jats:italic>. 1, 3 <jats:italic>vs<\/jats:italic>. 2; <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). <jats:styled-content style=\"fixed-case\">TUNEL<\/jats:styled-content>\u2010positive apoptotic cells were significantly higher in groups 2 and 3 compared with groups 1 and 4 (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). Necrosis was significantly more in group 4 than other groups (37.49% (4.68) <jats:italic>vs<\/jats:italic>. 19.97% (1.60) in <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>2; 20.36% (1.56) in <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>3; <jats:italic>P<\/jats:italic>\u00a0=\u00a00.006 for <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content> 2 <jats:italic>vs<\/jats:italic>. 4 and <jats:italic>P<\/jats:italic>\u00a0=\u00a00.019 for <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content> 3 <jats:italic>vs<\/jats:italic>. 4). Electron microscopy revealed numerous autophagosomes in groups 2 and 3 and mitochondrial damage and necrosis in group 4. The pancreatic and pulmonary myeloperoxidase activity in group 4 was significantly higher than that in the other groups (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01). Hence, severity of pancreatitis is a function of the dose of injurious agent, while inflammation is both dose and duration dependent, which may also explain the wide spectrum of severity of <jats:styled-content style=\"fixed-case\">AP<\/jats:styled-content> seen in clinical practice.<\/jats:p>","DOI":"10.1111\/iep.12081","type":"journal-article","created":{"date-parts":[[2014,4,25]],"date-time":"2014-04-25T06:52:40Z","timestamp":1398408760000},"page":"199-208","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Duration of injury correlates with necrosis in caerulein\u2010induced experimental acute pancreatitis: implications for pathophysiology"],"prefix":"10.1111","volume":"95","author":[{"given":"Tony G.","family":"Jacob","sequence":"first","affiliation":[{"name":"Department of Anatomy All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rahul","family":"Raghav","sequence":"additional","affiliation":[{"name":"Department of Psychiatry All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ajay","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pramod K.","family":"Garg","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tara S.","family":"Roy","sequence":"additional","affiliation":[{"name":"Department of Anatomy All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,4,25]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0411195"},{"key":"e_1_2_7_3_1","first-page":"156","volume-title":"Theory and Practice of Histological Techniques","author":"Bancroft J.D.","year":"2008"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199905063401807"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002689900204"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2004.tb00330.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00304.2003"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00140.2002"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2011.02.054"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1001\/archsurg.1993.01420170122019"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.mpa.0000246658.38375.04"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-199902000-00011"},{"key":"e_1_2_7_13_1","first-page":"2535","article-title":"VAMP8 Is the v\u2010SNARE that mediates basolateral exocytosis in a mouse model of alcoholic pancreatitis","volume":"118","author":"Cosen\u2010Binker L.I.","year":"2008","journal-title":"J. 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