{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T12:58:12Z","timestamp":1789909092463,"version":"4.0.1"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,8,24]],"date-time":"2015-08-24T00:00:00Z","timestamp":1440374400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"The Scientific Research Unit of Suleyman Demirel University","award":["SDU-BAP-2174-TU-10"],"award-info":[{"award-number":["SDU-BAP-2174-TU-10"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Experimental Path"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The aim of this study was to evaluate the histopathological and apoptotic changes occurring in the rat ipsilateral and contralateral testes, after experimental spermatic cord torsion, and to explore and the role of poly(<jats:styled-content style=\"fixed-case\">ADP<\/jats:styled-content>\u2010ribose) polymerase (<jats:styled-content style=\"fixed-case\">PARP<\/jats:styled-content>) cleavage in testicular torsion\u2013detorsion injury. A total of 37 Wistar albino rats were subjected to 720\u00b0 unilateral spermatic cord torsion for 1, 2 and 4\u00a0h, followed by 4\u2010h reperfusion, or else to a sham operation (control group). Histology of the testicle was evaluated using haematoxylin\u2013eosin (H&amp;E) staining and Johnsen's scoring system. Germ cell apoptosis was evaluated via active caspase\u20103 immunostaining, and <jats:styled-content style=\"fixed-case\">PARP<\/jats:styled-content> expression levels were evaluated via Western blotting. The mean Johnsen's tubular biopsy scores (<jats:styled-content style=\"fixed-case\">JTBS<\/jats:styled-content>) of the ipsilateral testicles were lower for all torsion groups than for the controls (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05), but the <jats:styled-content style=\"fixed-case\">JTBS<\/jats:styled-content> of the contralateral testicles were only lower in the 4\u2010h torsion group (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). The mean apoptosis score (<jats:styled-content style=\"fixed-case\">AS<\/jats:styled-content>) of the ipsilateral and contralateral testicles was significantly higher in the torsion groups than in the sham group. <jats:styled-content style=\"fixed-case\">AS<\/jats:styled-content> increased correlatively with torsion time, in both testicles. The effect of testicular torsion on <jats:styled-content style=\"fixed-case\">PARP<\/jats:styled-content> cleavage was time dependent, with the highest effect observed after 4\u00a0h of testicular torsion (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). Testicular torsion caused time\u2010dependent histological changes, apoptosis and increases in <jats:styled-content style=\"fixed-case\">PARP<\/jats:styled-content> cleavage. Our results suggest that testicular torsion\u2013detorsion injury caused cell damage and germ cell apoptosis that apparently involved cleavage of <jats:styled-content style=\"fixed-case\">PARP<\/jats:styled-content>. Increased <jats:styled-content style=\"fixed-case\">PARP<\/jats:styled-content> cleavage could, in turn, lead to enhanced apoptosis.<\/jats:p>","DOI":"10.1111\/iep.12137","type":"journal-article","created":{"date-parts":[[2015,8,25]],"date-time":"2015-08-25T04:06:13Z","timestamp":1440475573000},"page":"294-300","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["The efficiency of Poly(<scp>ADP<\/scp>\u2010Ribose) Polymerase (<scp>PARP<\/scp>) cleavage on detection of apoptosis in an experimental model of testicular torsion"],"prefix":"10.1111","volume":"96","author":[{"given":"P\u0131nar","family":"Aslan Ko\u015far","sequence":"first","affiliation":[{"name":"Faculty of Medicine Department of Medical Biology S\u00fcleyman Demirel University Isparta Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamdi","family":"Tuncer","sequence":"additional","affiliation":[{"name":"Faculty of Medicine 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